Marine microbes suppressed Vibrio and enhanced biological performance of euryhaline rotifer, Brachionus plicatilis.

The excessive use of antibiotics in mariculture has surpassed permitted levels, leading to their release into surrounding waters and accumulation in cultured organisms, which poses risks to human health and highlighting the urgent need for alternatives to reduce antibiotic use. Therefore, the present study aimed to test four microbes including Debaryomyces hansenii, Ruegeria mobilis, Lactobacillus plantarum and Bacillus subtilis, on lowering Vibrio, promoting population increase and survival of Brachionus plicatilis. The digestive enzymes activity including α-amylase, lipase and protease, microbial retention and biochemical composition of rotifers were analyzed.

Comprehensive analysis of the xbp1 gene in Pacific abalone Haliotis discus hannai: Structure, expression, and role in heat stress response.

The present study explores the x-box binding protein 1 (xbp1) gene in Haliotis discus hannai (Pacific abalone), focusing on its structure, expression, and functional role under heat stress. Southern blot revealed two copies of xbp1 in the intestine and mantle, one in the gill and muscle, and no detection in the digestive gland. mRNA expression level of xbp1 was highest in the gill, followed by the mantle, intestine, and muscle, with the digestive gland showing the lowest expression.

Highlighting antibiotic-free aquaculture by using marine microbes as a sustainable method to suppress Vibrio and enhance the performance of brine shrimp (Artemia franciscana).

Brine shrimp nauplii are widely used as live food in fish and shellfish aquaculture but they may transmit pathogenic Vibrio to the target species causing significant economic loss. Heavy usage of antibiotics is expensive and environmentally damaging. Use of natural microbes as probiotics for disease management is a more sustainable strategy.

Aquatic high iron induces hepatic ferroptosis in zebrafish (Danio rerio) via interleukin-22 signaling pathway.

Iron is one of the indispensable trace elements in living organisms. However, excessive iron deposition in organisms is prone to induce dysfunction of the liver and other vital organs. The present study aimed to investigate the mechanism how aquatic high iron affects iron transport and induces hepatic injury in zebrafish.

Novel Application of Skills for Psychological Recovery as an Early Intervention for Posttraumatic Stress Disorder, Depression, and Alcohol Misuse in Survivors of Recent Sexual Assault: A Case Series.

Skills for Psychological Recovery (SPR) is an evidence-informed, early intervention for teaching survivors coping skills in the aftermath of recent disasters. Although SPR has not been tested following recent sexual assault, there is theoretical support for applying SPR to the needs of recent sexual assault survivors. The current study is the first to describe the application of SPR among survivors of recent sexual assault.

Oral probiotic extracellular vesicle therapy mitigates Influenza A Virus infection via blunting IL-17 signaling.

The influenza A virus (IAV) damages intestinal mucosal tissues beyond the respiratory tract. Probiotics play a crucial role in maintaining the balance and stability of the intestinal microecosystem. Extracellular vesicles (EVs) derived from probiotics have emerged as potential mediators of host immune response and anti-inflammatory effect.

The Effectiveness of Afatinib as First-Line Treatment in Vietnamese Patients With EGFR-Mutant Non-Small Cell Lung Cancer and Brain Metastases.

Introduction: The role of afatinib in the first-line treatment of EGFR-mutant advanced non-small cell lung cancer (NSCLC) patients has been proven through clinical trials and real-world studies. However, additional data on the effectiveness of afatinib in patients with brain metastases are lacking.

Methods: EGFR-mutant NSCLC patients with brain metastases were retrospectively reviewed across nine cancer centers in Vietnam from April 1, 2018 to June 1, 2022.

The Molecular Mechanism of Farnesoid X Receptor Alleviating Glucose Intolerance in Turbot ().

To explore the molecular targets for regulating glucose metabolism in carnivorous fish, the turbot () was selected as the research object to study. Farnesoid X receptor (FXR; NR1H4), as a ligand-activated transcription factor, plays an important role in glucose metabolism in mammals. However, the mechanisms controlling glucose metabolism mediated by FXR in fish are not understood.

Supplementation of recombinant human lysozyme into diets affects the growth performance, muscle quality, immunity and intestinal microbiota in large yellow croaker Larimichthyscrocea.

The aim of the present study was to investigate the influence of supplementation of lysozyme (LZM) into diet on the growth performance, muscle quality, immunity, intestinal microbiota in large yellow croaker (Larimichthys crocea) with initial body weight of 194.5 ± 0.27 g.

Increase in PUFA and protein, and decrease in carbohydrate intake improves liver fat in 12 months and the role of weight loss as a mediator: A randomized controlled trial.

Background & Aims: Recently, a beneficial effect of high intake of unsaturated fatty acids (UFA) and protein on intrahepatic lipids (IHL) was demonstrated over 12 months within a randomized controlled trial (the NutriAct trial). We now aimed to explore the specific macronutrient components driving this IHL improvement within this trial in middle-aged and elderly subjects (50-80 y) at risk for age-related diseases.

Methods: The NutriAct trial (n = 502) analyzed the effect of a high-protein and high-UFA diet on age related diseases including fatty liver disease.

Reconfiguring Alginate-Based Supra-amphiphiles via Redox-Responsive Pickering Emulsions for Enhancing Pesticide Droplet Retention.

In view of the micro/nanostructure tunability of colloid particles, they acted as agrichemical delivery systems. Herein, supra-amphiphilic colloids were fabricated using a host-guest interfacial recognition-architected emulsion template strategy and controlled their agricultural interfacial interactions with the superhydrophobic target surface. Interestingly, the supramolecular interfacial system possessed redox-stimulated molecular reconfigurability by regulating aggregation/disaggregation behaviors.

Phosphatidylethanolamine exerts anti-inflammatory action by regulating mitochondrial function in macrophages of large yellow croaker (Larimichthys crocea).

Phosphatidylethanolamine (PE) is a ubiquitous bioactive lipid in cells, which participates in regulating many metabolic processes. Exogenous PE has been reported to play a positive regulatory role in macrophage inflammatory responses. However, the molecular mechanisms of PE in regulating macrophage inflammation are not completely understood.

Dietary Oryzanol (Ory) Improved the Survival and Growth of Large Yellow Croaker () Larvae via Promoting Activities of Digestive Enzymes, Antioxidant Capacity, and Lipid Metabolism.

A feeding study lasting 30 days was carried out to determine the effects of dietary Oryzanol (Ory) on the survival, growth, antioxidant capacity, peptic enzymes, as well as lipid metabolism of larvae (11.87 ± 0.59 mg) using four different Ory concentrations in microfeed formulations (0, 20, 40, and 80 mg/kg), all preserving isolipidic (18.

The Combination of Host-Associated R32 and Stachyose Promotes the Intestinal Health of Turbot (. L).

An 8-week feeding trial was conducted to investigate the effects of host-associated R32 and stachyose on the intestinal mucosal defense system of turbot (. L). Three isonitrogenous and isolipidic diets were formulated: control diet (C), control diet with 1.

Tip60-mediated Rheb acetylation links palmitic acid with mTORC1 activation and insulin resistance.

Excess dietary intake of saturated fatty acids (SFAs) induces glucose intolerance and metabolic disorders. In contrast, unsaturated fatty acids (UFAs) elicit beneficial effects on insulin sensitivity. However, it remains elusive how SFAs and UFAs signal differentially toward insulin signaling to influence glucose homeostasis.

Protective potential of selected microbial and non-microbial biostimulants against leaf blotch in winter wheat as affected by the form of N supply.

Introduction: The production of high-quality food for the growing world population on the one hand and the reduction of chemical-synthetic pesticides on the other hand represents a major challenge for agriculture worldwide. The effectiveness of a combination of microbial and non-microbial biostimulants (BSs) with various nitrogen (N) forms in pathogen defense is discussed as a promising, but still poorly understood bio-based alternative for crop protection.

Methods: For this reason, nitrate and stabilized ammonium fertilizer both combined with a consortium of , , and as soil treatment or with a mixture of seaweed extract () together with chitosan-amended micronutrient fertilizer as foliar spray application were compared under controlled greenhouse conditions.

Accurate locomotor activity profiles of group-housed mice derived from home cage monitoring data.

Introduction: Holistic phenotyping of rodent models is increasing, with a growing awareness of the 3Rs and the fact that specialized experimental setups can also impose artificial restrictions. Activity is an important parameter for almost all basic and applied research areas involving laboratory animals. Locomotor activity, the main form of energy expenditure, influences metabolic rate, muscle mass, and body weight and is frequently investigated in metabolic disease research.

[Progress in application of machine learning in epidemiology].

Population based health data collection and analysis are important in epidemiological research. In recent years, with the rapid development of big data, Internet and cloud computing, artificial intelligence has gradually attracted attention of epidemiological researchers. More and more researchers are trying to use artificial intelligence algorithms for genome sequencing and medical image data mining, and for disease diagnosis, risk prediction and others.

FABP1 induces lipogenesis by regulating the processing of SREBP1 in hepatocytes of large yellow croaker (Larimichthys crocea).

Fatty acid-binding protein 1 (FABP1) plays an important role in regulating fatty acid metabolism in liver, which is a potential therapeutic target for diseases such as non-alcoholic fatty liver disease (NAFLD). However, the underlying mechanisms are not well defined. Using complementary experimental models, we discovered FABP1 induction in hepatocytes as a primary mediator of lipogenesis when exposed to fatty acids, especially saturated fatty acids (SFAs).

The Effect of Individual Attitude toward Healthy Nutrition on Adherence to a High-UFA and High-Protein Diet: Results of a Randomized Controlled Trial.

Unlabelled: Despite beneficial cardiovascular effects, substantial long-term modulation of food pattern could only be achieved in a limited number of participants. The impact of attitude towards healthy nutrition (ATHN) on successful modulation of dietary behavior is unclear, especially in the elderly. We aimed to analyze whether the personal ATHN influences 12-month adherence to two different dietary intervention regimes within a 36-month randomized controlled trial.

Fabrication of Flexible and Re-entrant Liquid-Superrepellent Surface Using Proximity and PNIPAM-Assisted Soft Lithography.

The nature-inspired flexible and re-entrant liquid-superrepellent surface has attracted significant attention due to its excellent superomniphobic performance against low-surface-tension liquids. Although conventional photolithography and molding methods offer the advantage of large-area manufacturing, they often involve multiple double-sided alignment and exposure steps, resulting in complex procedures with long processing cycles. In this study, we proposed a straightforward single-exposure ultraviolet proximity lithography method for re-entrant liquid-superrepellent surface fabrication using a photomask with a coaxial circular aperture and ring.