Optimization of germination, callus induction, and cell suspension culture of African locust beans (Jacq.) Benth.

The present study was carried out to determine the best pre-sowing treatments that can enhance the germination and seedling growth of (Jacq.) Also, to establish and long-term maintenance of calli and cell suspension cultures . The result of various pre-sowing treatments showed that seeds soaked in concentrated HSO treatment appeared the highest germination percentage, higher value of plant height, number of leaves, number of branches and stem girth.