Statistical optimization of hyaluronidase production by Ochrobactrum intermedium MEFS strain along with in vivo application in the treatment of aesthetic filler complications.

This study aims to optimize the production of microbial hyaluronidase (hyase) from Ochrobactrum intermedium MEFS strain using a statistical model and assess its effectiveness in degrading dermal filler in hairless mice. Plackett-Burman design identified nicotinic acid and CaBr as the main factors affecting hyase activity, with optimal concentrations of 5.51 μg/mL for nicotinic acid and 99.

Purification and characterization of the produced hyaluronidase by Brucella Intermedia MEFS for antioxidant and anticancer applications.

Hyaluronidase (hyase) is an endoglycosidase enzyme that degrades hyaluronic acid (HA) and is mostly known to be found in the extracellular matrix of connective tissues. In the current study, eleven bacteria isolates and one actinomycete were isolated from a roaster comb and screened for hyase production. Seven isolates were positive for hyase, and the most potent isolate was selected based on the diameter of the transparent zone.