Carrier erythrocytes: recent advances, present status, current trends and future horizons.

Introduction: Carrier erythrocytes, thanks to their main advantages, including biocompatibility, biodegradability, immunocompatibility, simple and well-known structure and physiology, availability for sampling and versatility in loading and use, have been studied as cellular carriers for delivery of drugs and other bioactive agents for more than three decades. Based on this body of knowledge and recent advances in this field, and with the help of novel multidisciplinary sciences and technologies, it seems that this field is becoming renowned and experiencing an outstanding turning point in its developmental history.

Areas Covered: In this trendy and timely review, following a short historical review of the story of erythrocytes from oxygen delivery to drug delivery and evaluation of the present status of these biocarriers, recent advances and current experimental, technological and clinical trends, as well as future horizons, and, in particular, translation-prone strategies, are going to be discussed in detail.

Preparation and in-vitro characterization of tramadol-loaded carrier erythrocytes for long-term intravenous delivery.

Objectives: The hypo-osmotic dialysis method was used for preparation of tramadol-loaded human intact erythrocytes. In response to rapid drug escape from the erythrocytes, a membrane cross-linker, glutaraldehyde, was used successfully.

Methods: The resulting carrier cells were validated in terms of the accuracy and precision of the whole drug loading procedure.

Novel delivery systems for interferons.

Interferons, IFNs, are among the most widely studied and clinically used biopharmaceuticals. Despite their invaluable therapeutic roles, the widespread use of IFNs suffers from some inherent limitations, mainly their relatively short circulation lifespan and their unwanted effects on some non-target tissues. Therefore, both these constraints have become the central focus points for the research efforts on the development of a variety of novel delivery systems for these therapeutic agents with the ultimate goal of improving their therapeutic end-points.

Lipoproteins: from physiological roles to drug delivery potentials.

Lipoproteins, the endogenous lipid-protein associations responsible for lipid metabolism within the human body, have attracted interest in recent years for their potential as drug delivery carriers owing to, mainly, their lipophilic/amphiphilic nature, which makes them ideal for interacting with highly lipophilic drugs. After lipoprotein particles have been isolated from the blood, drugs can be "loaded" onto them with a variety of methods. Loading can be done either in soluble/suspended form in a liquid medium or as a dry film.

Applications of carrier erythrocytes in delivery of biopharmaceuticals.

Carrier erythrocytes, resealed erythrocytes loaded by a drug or other therapeutic agents, have been exploited extensively in recent years for both temporally and spatially controlled delivery of a wide variety of drugs and other bioactive agents owing to their remarkable degree of biocompatibility, biodegradability and a series of other potential advantages. Biopharmaceuticals, therapeutically significant peptides and proteins, nucleic acid-based biologicals, antigens and vaccines, are among the recently focused pharmaceuticals for being delivered using carrier erythrocytes. In this article, the potential applications of erythrocytes in drug delivery have been reviewed with a particular stress on the studies and laboratory experiences on successful erythrocyte loading and characterization of the different classes of biopharmaceuticals.

A simple and rapid HPLC method for quantitation of interferon-alpha2b in dosage forms and delivery systems.

Development of reliable assay methods for quantitation of interferons in dosage forms has encountered serious limitations because of the physicochemical nature of these proteins as well as the sensitivity/selectivity issues. A rapid, available, and easy-to-use reversed-phase HPLC method has been developed for quantitative analysis of interferon-alpha2b in pharmaceuticals. The reversed- phase method was based on a gradient system using a wide-pore C4 column and produced linear response in drug concentration range of 0.