Optimization of food-grade medium for co-production of bioactive substances by LA-5 for explaining pharmabiotic mechanisms of probiotic.

This study aimed to optimize the co-production of conjugated linoleic acid (CLA), exopolysaccharides (EPSs) and bacteriocins (BACs) by LA-5 in dairy food-grade by-product. The factorial design revealed that the significant factors were temperature, time, and yeast extract. Then the response surface methodology was used for optimization.

Microencapsulation of LA-5 and BB-12 in pectin and sodium alginate: A comparative study on viability, stability, and structure.

The present study aimed at examining whether the microencapsulation of LA-5 and BB-12 inside hydrogels could prolong their survival in freeze-drying conditions, stored at 4℃ and in the gastrointestinal medium. Microencapsulation was performed by emulsion with a syringe, while sodium alginate and high methoxyl pectin were used as a carrier material. A relatively high efficiency of encapsulation was obtained (>92%).

Optimization of bacterial cellulose production by PTCC 1734 in a low-cost medium using optimal combined design.

This study was aimed to optimize the production of bacterial cellulose (BC) by PTCC 1734 using mixture of date syrup and cheese whey as carbon sources as well as ascorbic acid as a supplementary agent and to characterize the properties of produced BC. The results showed the highest BC production on the 10th day. The 50:50 ratio of date syrup and cheese whey lead to the highest BC production.

Use of bacterial cellulose film modified by polypyrrole/TiO-Ag nanocomposite for detecting and measuring the growth of pathogenic bacteria.

The aim of this study was to use of bacterial cellulose/polypyrrole/TiO-Ag (BC/PPy/TiO-Ag) nanocomposite film to detect and measure the growth of 5 pathogenic bacteria. For this purpose, at first, 13 BC/PPy/TiO-Ag films were fabricated, then bacterial suspensions were prepared according to McFarland standard. The results showed that by increasing the bacterial concentration, the electrical resistance of sensors was decreased and there was a relation between bacterial concentration and bacterial type with electrical resistance change of sensors.

Numerical optimization of probiotic Ayran production based on whey containing transglutaminase and gel.

The purpose of this study was to optimize the functional properties of probiotic Ayran. Two-level fractional factorial design with four center points was used to investigate the effect of five independent variables including, reconstructed whey protein (70-90% of milk), salt (0.5-1 g/100 g), gel (0-30 g/100 g), transglutaminase enzyme (0-14 unit/100 g) and storage time (1-21 days).

Optimization and characterization of bacterial cellulose produced by Komagatacibacter xylinus PTCC 1734 using vinasse as a cheap cultivation medium.

In the present study, vinasse was used to produce bacterial cellulose (BC) by Komagatacibacter xylinus PTCC 1734. Central composite design (CCD) was utilized to evaluate the effects of vinasse concentration (%) and incubation time (Day) on different responses such as thickness as well as wet and dry weights of the produced BC membrane. The increase of vinasse concentration and incubation time caused an increase in the wet weight of BC; however, thickness decreased by increasing incubation time.

New formulation of vitamin C encapsulation by nanoliposomes: production and evaluation of particle size, stability and control release.

In the present study, the effects of different ratios of milk phospholipids, cholesterol and phytosterols (Campesterol) powder (50-100%, 0-50%, and 0-50%, respectively) and sonication time (20, 25, 30, 35 and 40 min) were investigated to produce a new formulation of nanoliposomes for encapsulation of vitamin C. The results showed that increasing the time of sonication and decreasing the ratio of phospholipid to phytosterol significantly decreased nanoliposomes' particle size ( < 0.05).

Exopolysaccharides production by Lactobacillus acidophilus LA5 and Bifidobacterium animalis subsp. lactis BB12: Optimization of fermentation variables and characterization of structure and bioactivities.

In this study, the most important variables (incubation temperature, fermentation time and yeast extract concentration) responsible for the exopolysaccharides (EPSs) production by Lactobacillus acidophilus LA5 and Bifidobacterium animalis subsp. lactis BB12 were screened. The EPSs synthesize by LA5, BB12, and their co-culture were successfully optimized and were 349.

Affecting parameters on fabrication of β-D-galactosidase immobilized chitosan/poly (vinyl alcohol) electrospun nanofibers.

β-galactosidase was successfully immobilized onto chitosan (CS)/polyvinyl alcohol (PVA) blend nanofibers through electrospinning. The effects of both polymer solution parameters and the electrospinning process conditions on the morphology of nanofibers and enzyme activity were studied. FESEM results showed that both morphology and diameter of the nanofibers were significantly influenced by the studied factors.