Laboratory validation of patient-specific templating for total knee arthroplasty.

Patient-specific templating (PST), which is a sister procedure to patient-specific instrumentation (PSI) but hospital-based, is relatively less complex and less expensive than robotics and navigation. However, there are some concerns about the PST including the process of preoperative planning, 3D printing and material, positioning of PST intraoperatively, availability, and clinical value. The purpose of this study was to validate the technical accuracy and reliability of the PST technique in the lab and to report the outcomes of clinical application.

Successful Treatment of an Atypical Presentation of Disseminated Retroperitoneal Seminoma: A Case Report.

Background: Testicular seminoma is the most common malignant tumor of the testis. It occurs at a rate of 5 per 100 000 men, primarily between the ages of 15 to 34. While seminomas typically occur in the testis, other primary sites include the mediastinum, the retroperitoneum, or other extra-gonadal sites.

Pelvic flexion/extension and its impact on functional acetabular alignment and stability following total hip replacement.

Purpose: Dislocation following total hip arthroplasty (THA) remains a significant clinical problem and can occur even with optimal implant alignment. We hypothesized that different patterns of pelvic flexion/extension (PFE) during daily activities may influence acetabular alignment and contribute to impingement and instability following THA. Recently, there has been an increased interest in spinopelvic alignment and its impact on THA.

Schiff Base Reaction in a Living Cell: In Situ Synthesis of a Hollow Covalent Organic Polymer To Regulate Biological Functions.

Artificially performing chemical reactions in living biosystems to attain various physiological aims remains an intriguing but very challenging task. In this study, the Schiff base reaction was conducted in cells using Sc(OTf) as a catalyst, enabling the in situ synthesis of a hollow covalent organic polymer (HCOP) without external stimuli. The reversible Schiff base reaction mediated intracellular Oswald ripening endows the HCOP with a spherical, hollow porous structure and a large specific surface area.

The difference in leg lengths following total knee replacement for patients with severe osteoarthritic deformity.

Purpose: Increased height after total knee replacement surgery (TKR) may offer patients higher satisfaction as well as the quality of life. Therefore, in this paper, we aim to document the changes in leg length after TKR in patients with severe bilateral deformities.

Methods: The data of 61 patients were collected from the Egyptian Community Arthroplasty Register; of them, 21 patients had unilateral TKR while 40 had bilateral simultaneous TKR.

Learning curve of the 67 steps of conventional total knee replacement: an experimental study.

Unlabelled: The instrumentation system for total knee replacement (TKR) has been there since the 1970s. The many steps and instruments are the main features despite several modifications over the last 50 years. This may lead to the accumulation of errors as certain steps are dependent on others.

The rate and management of prosthetic joint infection in the low-income setting: a cross-sectional study.

Unlabelled: Although the rate of prosthetic joint infection (PJI) after total hip and knee arthroplasties (THA and TKA, respectively) is well documented in developed countries, there is a paucity of information in the literature on infection rates in low-and-middle-income countries. This study aims to review the PJI rate and management based on the Egyptian Community Arthroplasty Registry (ECAR) and six arthroplasty surgeons.

Methods: Using data from the ECAR, for over 10 years, and surveying six high-volume arthroplasty surgeons, we reviewed the infection rate, common organisms, antibiotics used, and how the revision surgeries were done.

Dual-Modal Apoptosis Assay Enabling Dynamic Visualization of ATP and Reactive Oxygen Species in Living Cells.

ATP and reactive oxygen species (ROS) are considered significant indicators of cell apoptosis. However, visualizing the interplay between apoptosis-related ATP and ROS is challenging. Herein, we developed a metal-organic framework (MOF)-based nanoprobe for an apoptosis assay using duplex imaging of cellular ATP and ROS.

Hepatitis E virus (HEV) open reading frame 2: Role in pathogenesis and diagnosis in HEV infections.

Hepatitis E virus (HEV) infection occurs worldwide. The HEV genome includes three to four open reading frames (ORF1-4). ORF1 proteins are essential for viral replication, while the ORF3 protein is an ion channel involved in the exit of HEV from the infected cells.

AuNPs-COFs Core-Shell Reversible SERS Nanosensor for Monitoring Intracellular Redox Dynamics.

The redox homeostasis in living cells is greatly crucial for maintaining the redox biological function, whereas accurate and dynamic detection of intracellular redox states still remains challenging. Herein, a reversible surface-enhanced Raman scattering (SERS) nanosensor based on covalent organic frameworks (COFs) was prepared to dynamically monitor the redox processes in living cells. The nanosensor was fabricated by modifying the redox-responsive Raman reporter molecule, 2-Mercaptobenzoquione (2-MBQ), on the surface of gold nanoparticles (AuNPs), followed by the in situ coating of COFs shell.

Label-free detection of DNA methylation by surface-enhanced Raman spectroscopy using zirconium-modified silver nanoparticles.

DNA methylation is an important feature of gene epigenetics that affects the metabolic process of organisms. Although surface-enhanced Raman spectroscopy (SERS) has demonstrated great potential in label-free DNA detection, discriminating the various processes involved in DNA methylation remains a challenge. DNA molecules fold themselves, wrapping the hydrophobic bases, thus making it difficult for traditional methods to detect single-base signals.

Revealing the Catalytic Activities of Single-Dispersed Pd Clusters Embedded on Hollow Nanoparticles toward the Hydrogen Evolution Reaction.

Hydrogen evolution reaction (HER) is one of the revolutionary aspects that grab lots of attention for the production of clean energy sources, increasing the demands of revealing the intrinsic activities of HER catalysts for designing efficient candidates. Based on using only surface active sites on solid nanoparticles (SNPs), catalysts with high atom dispersion are immensely desirable to magnify their efficiency through maximum atom utilization. Herein, we employed hollow mesoporous silica nanoparticles (HMSNPs) as an insulating nanoplatform for engineering single-dispersed Pd clusters on their surface, assuring high dispersion of the Pd clusters.

Clinical Infections, Antibiotic Resistance, and Pathogenesis of .

) constitutes the main part of the human skin microbiota. It is widespread in hospitals and among medical staff, resulting in being an emerging microbe causing nosocomial infections. , especially strains that cause nosocomial infections, are more resistant to antibiotics than other coagulase-negative Staphylococci.

Reversible polymerization of carbon dots based on dynamic covalent imine bond.

Carbon dots (CDs), as new type of carbon-based nanoparticles, are considered to be an aggregate with irreversible polymerization. Achieving the reversible tunability of CDs luminescence based on their reversible polymerization is a challenging subject. Herein, we, for the first time, design and construct the blue-emitting CDs with reversible polymerization by a room-temperature Schiff base reaction between tannic acid and ethylenediamine.

"Anosmia" the mysterious collateral damage of COVID-19.

COVID-19 pandemic spreads worldwide, with more than 100 million positive cases and more than 2 million deaths. From the beginning of the COVID-19 pandemic, several otolaryngologists described many cases of a sudden loss of smell (anosmia) associated with the disease with or without additional symptoms. Anosmia is often the first and sometimes the only sign in the asymptomatic carriers of COVID-19.

Dynamic Visualization of Endoplasmic Reticulum Stress in Living Cells via a Two-Stage Cascade Recognition Process.

The endoplasmic reticulum (ER) is crucial for the regulation of multiple cellular processes, such as cellular responses to stress and protein synthesis, folding, and posttranslational modification. Nevertheless, monitoring ER physiological activity remains challenging due to the lack of powerful detection methods. Herein, we built a two-stage cascade recognition process to achieve dynamic visualization of ER stress in living cells based on a fluorescent carbon dot (CD) probe, which is synthesized by a facile one-pot hydrothermal method without additional modification.

Dual-Emitting Carbonized Polymer Dots Synthesized at Room Temperature for Ratiometric Fluorescence Sensing of Vitamin B12.

Ratiometric fluorescence (FL) probes are highly desirable for highly sensitive and reliable assays. Dual-emitting carbonized polymer dots (CPDs) have great application prospects in building ratiometric FL sensors. However, dual-emitting CPDs are usually synthesized at high temperatures and high pressures, which not only increases the cost but also complicates the structure of CPDs.

In Situ Monitoring of Hydrogen Peroxide Released from Living Cells Using a ZIF-8-Based Surface-Enhanced Raman Scattering Sensor.

Hydrogen peroxide (HO) widely involves in intracellular and intercellular redox signaling pathways, playing a vital role in regulating various physiological events. Nevertheless, current analytical methods for the HO assay are often hindered by relatively long response time, low sensitivity, or self-interference. Herein, a zeolitic imidazolate framework-8 (ZIF-8)-based surface-enhanced Raman scattering (SERS) sensor has been developed to detect HO released from living cells by depositing ZIF-8 over SERS active gold nanoparticles (AuNPs) grafted with HO-responsive probe molecules, 2-mercaptohydroquinone.

Comparison of Functional Outcome of Total and Unicompartmental Knee Arthroplasty Using Computer-Assisted Patient-Specific Templating.

Background: Knee arthroplasty surgeries are in ever-increasing demand. With unicompartmental knee arthroplasty (UKA), patients may benefit from a higher range of flexion and a better Knee Society Score (KSS).

Aim: In this study, we compared the short-term clinical outcomes of total knee arthroplasty (TKA) and UKA using the patient-specific templating (PST) technique.

Satisfactory Outcome for a Challenging Case of Infected Native Pelvic Discontinuity with Significant Bone Loss: A Case Report.

Case: This is a challenging case of infected native pelvic discontinuity with massive acetabular bone loss. The infection was persistent despite repeated debridement. A new technique of articulated antibiotic spacer plus Mexican hat antibiotic spacer has been used.

Electrochemically renewable SERS sensor: A new platform for the detection of metabolites involved in peroxide production.

The accurate detection of hydrogen peroxide (HO)-involved metabolites plays a significant role in the early diagnosis of metabolism-associated diseases, whereas most of current metabolite-sensing systems are often hindered by low sensitivity, interference of coexisting species, or tedious preparation. Herein, an electrochemistry-regenerated surface-enhanced Raman scattering (SERS) sensor was developed to serve as a universal platform for detecting HO-involved metabolites. The SERS sensor was constructed by modifying newly synthesized 2-mercaptohydroquinone (2-MHQ) molecules on the surface of gold nanoparticles (AuNPs) that were electrochemically predeposited on an ITO electrode.

The W.A.I.O.T. Definition of Peri-Prosthetic Joint Infection: A Multi-center, Retrospective Validation Study.

Peri-prosthetic joint infection (PJI) definition plays an important role in diagnostic and therapeutic decisions. However, while several criteria have been proposed by eminent institutions to define a PJI in the last decade, their clinical validation has been rarely performed. Aim of the present multicenter, international, retrospective study was to validate the World Association against Infection in Orthopedics and Trauma (WAIOT) pre/intra-operative PJI definition with post-operative confirmatory tests.

Facile route for C-N/NbO nanonet synthesis based on 2-methylimidazole for visible-light driven photocatalytic degradation of Rhodamine B.

Herein, we fabricated a C and N co-modified NbO nanonet structure (C-N/NbONNs) from niobium oxalate using 2-methylimidazole (Hmim) as a source for C and N a simple hydrothermal route. The obtained nanonets are robust and cost-effective with excellent recycling stability. Compared with N-doped TiO (N-TiO) and a NbO control sample (NbO-CS), the resulting nanonets exhibited the highest performance toward the photocatalytic degradation of Rhodamine B (RhB) upon visible light irradiation ( > 420 nm).

Correlated Anodic-Cathodic Nanocollision Events Reveal Redox Behaviors of Single Silver Nanoparticles.

We reported a novel method to real-time monitor the redox behaviors of single Ag nanoparticles (AgNPs) at a Au ultramicroelectrode between oxidizing and reducing pulse potentials using the nanocollision electrochemical method. At fast pulse potentials, the instantaneous anodic-cathodic current transients of a single AgNP were observed for the electrooxidation of AgNP, followed by the electroreduction of the newborn silver oxide (AgO) NP in alkaline media via switching of redox potentials; however, only anodic oxidation signals of individual AgNPs were observed in neutral solution. Through this study, we have revealed the substantial different dynamic nanocollision electrochemical behaviors of single AgNPs on the electrode surface in various media.