Publications by authors named "Mahmoud A Wahb-Allah"
Soil salinity and the scarcity of freshwater resources are two of the most common environmental constraints that negatively affect plant growth and productivity worldwide. The tomato ( Mill.) plant is moderately sensitive to salinity.
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- The study focuses on creating a mutant population of cucumbers to explore gene functions related to important traits.
- Researchers generated 3,331 mutant seed families using two concentrations of EMS and detected 26 mutations in specific genes, revealing an average mutation rate of 1/1147 Kb.
- Notably, while some mutations did not affect sexual development in plants, a specific mutation led to a significant change in sexual expression, highlighting TILLinG as a useful technique for crop genetic studies.
Targeting Induced Local Lesions IN Genomes (TILLING) combines chemical mutagenesis with high throughput screening to allow the generation of alleles of selected genes. In this study, TILLING has been applied to produce a series of mutations in genes encoding essential components of the tomato light signal transduction pathway in an attempt to enhance fruit nutritional quality. Point mutations to DEETIOLATED1 (DET1), which is responsible for the high pigment2 (hp2) tomato mutant, resulted in elevated levels of both carotenoid and phenylpropanoid phytonutrients in ripe fruit, whilst immature fruit showed increased chlorophyll content, photosynthetic capacity and altered fruit morphology.
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