The role of fatty acids on ICSI outcomes: a prospective cohort study.

Background: Our objective was to determine the effect of fatty acids (FAs) in serum and follicular fluid (FF) on fertilization and intracytoplasmic sperm injection (ICSI) outcomes.

Methods: One hundred five women aged 18-38 years undergoing ICSI were recruited in this prospective cohort study. oocyte and emberyo quality was morphologically assessed.

Determination of Glucose and Cholesterol Using a Novel Optimized Luminol- CuO Nanoparticles-H2O 2 Chemiluminescence Method by Box-Behnken Design.

In this study, a simple, rapid and sensitive luminol-CuO nanoparticles-H2O2 chemiluminescence (CL) method has been proposed for determination of glucose and cholesterol in plasma. Response surface methodology (RSM) based on Box-Behnken design (BBD) was used to optimize the most important operating variables (solution pH effect and the CL reagents concentration) of luminol CL system. In optimum conditions, it was found that CuO nanoparticles (NPs) could enhance the CL intensity and the method sensitivity towards evaluation of trace amount of glucose and cholesterol.

Determination of cysteine and glutathione based on the inhibition of the dinuclear Cu(II)-catalyzed luminol-H2O2 chemiluminescence reaction.

The catalyzed luminol chemiluminescent reaction has received a great amount of attention because of its high sensitivity and low background signal which make the reaction an attractive analytical chemistry tool. The present study, introduces the beneficial catalytic effects of dinuclear Cu(II) complex [Cu2L2(TAE)]X2, where TAE=tetraacetylethane; L=N,N(')-dibenzylethylenediamine and X=ClO4 on the luminol chemiluminescent reaction as a novel probe for the determination of glutathione (GSH) and L-cysteine (CySH) in human serum and urine. The [Cu2L2(TAE)]X2 has exhibited highly efficient catalytic activity of luminol CL as an artificial peroxidase model at pH as low as 7.