Publications by authors named "Mahdi Assari"
Article Synopsis
- - N1-methyladenosine (mA) is a common chemical modification found in tRNAs across various organisms, playing a crucial role in stabilizing their structure and regulating translation.
- - The study introduces a new method called templated-ligation followed by quantitative PCR (TL-qPCR) to accurately measure mA levels in specific tRNAs, filling a gap in existing measurement techniques.
- - This TL-qPCR method utilizes a special ligase to combine DNA oligonucleotides and can quantify mA levels using very small amounts of RNA, facilitating further research into the dynamics and significance of mA modifications in human cells.
Here, we report the design, construction, and characterization of a tRNA neochromosome, a designer chromosome that functions as an additional, de novo counterpart to the native complement of Saccharomyces cerevisiae. Intending to address one of the central design principles of the Sc2.0 project, the ∼190-kb tRNA neochromosome houses all 275 relocated nuclear tRNA genes.
View Article and Find Full Text PDFTransfer RNA (tRNA) utilizes multiple properties of abundance, modification, and aminoacylation in translational regulation. These properties were typically studied one-by-one; however, recent advance in high throughput tRNA sequencing enables their simultaneous assessment in the same sequencing data. How these properties are coordinated at the transcriptome level is an open question.
View Article and Find Full Text PDFHere we discuss "hidden variables", which are typically introduced during an experiment as a consequence of the application of two independent variables together to create a stimulus. With increased sophistication in modern chemical biology tools and related precision interrogation techniques, hidden variables have become integral to many chemical biologists' routine experiments. For instance, they can appear in the use of light-activatable chemical probes (e.
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