Luliconazole-loaded nanostructured lipid carrier: formulation, characterization, and in vitro antifungal evaluation against a panel of resistant fungal strains.

Luliconazole (LCZ) is a topical imidazole antifungal agent with broad-spectrum activity. However, LCZ encounters challenges such as low aqueous solubility, skin retention, and penetration, which reduce its dermal bioavailability and hinder its efficacy in drug delivery. The aim of the present study was to formulate, characterize, and evaluate the in vitro antifungal efficacy of luliconazole-loaded nanostructured lipid carriers (LCZ-NLCs) against a panel of resistant fungal strains.

Molecular epidemiology of dermatophytosis in Golestan, Iran: A cross-sectional study.

Keratinophilic fungi, or dermatophytes, are recognized as the predominant fungal agents responsible for superficial skin diseases globally. The identification of species of dermatophytes is crucial for both therapeutic and epidemiological considerations. The primary objective of the present study was to investigate the epidemiology of dermatophytosis among patients who sought medical attention at the medical mycology laboratory in Golestan province.

In vitro activity of nanoliposomal amphotericin B against terbinafine-resistant Trichophyton indotineae isolates.

Background: The emergence of resistance in dermatophytes underscores the necessity for developing novel and alternative treatment options.

Methods: The present study aimed to evaluate the in vitro activity of nanoliposomal amphotericin B against a large panel of terbinafine-resistant Trichophyton indotineae isolates. In vitro susceptibility testing of nanoliposomal amphotericin B and comparators against 50 clinical isolates of terbinafine-resistant T.

Undiagnosed hypertension and diabetes mellitus in the Tabari cohort: a population-based study.

Background: Undiagnosed hypertension (HTN) and diabetes mellitus (DM) are major public health challenges worldwide. We aimed to investigate the prevalence of undiagnosed HTN and DM, and their correlates, in a large-scale population-based cohort study.

Methods: In this cross-sectional study, data collected during the enrollment phase of the Tabari Cohort study (TCS) were utilized.

In vitro and silico activity of piperlongumine against azole-susceptible/resistant Aspergillus fumigatus and terbinafine-susceptible/resistant Trichophyton species.

In recent years, the widespread emergence of drug resistance in yeasts and filamentous fungi to existing antifungal armamentariums has become a severe threat to global health. There is also concern regarding increased rates of azole resistance in Aspergillus fumigatus and Terbinafine resistance in Trichophyton species. To overcome this concern of resistance to regular therapies, new antifungal drugs with novel and effective mechanisms are crucially needed.

Identification of fungal agents isolated from burn lesions using mycological and molecular methods in patients admitted to Velayat burn hospital in Rasht city during 2022-2023.

Background And Objectives: Fungal burn wound infections (FBWIs) are one of the most disastrous complications in burn patients. The present study investigated the incidence and the species distribution of fungal agents isolated from burn lesions and reviewed the feautures, underlying conditions, and outcomes of patients.

Materials And Methods: The wounds were swabbed and cultured on Sabouraud Dextrose Agar with chloramphenicol medium.

Metabolic Patterns of Fluconazole Resistant and Susceptible Clade V and I.

, an emerging non- multidrug-resistant yeast, has become a significant cause of invasive candidiasis in healthcare settings. So far, data on the metabolites of in different clades are minimal, and no studies have focused on clade V metabolites. Therefore, Gas chromatography-mass spectrometry (GC-MS) was used for the metabolomic profiling of clade I compared with fluconazole-resistant and susceptible in clade V strains.

Multi-state evaluation of Candida infections in burn patients.

Background: Burn patients are at high risk of developing secondary invasive fungal infections due to their compromised skin barrier, extensive use of antibiotics, and immunosuppression.

Objectives: We investigated demographic characteristics and clinical factors associated with Candida infections in intensive care unit (ICU) burn patients, and the in vitro antifungal susceptibility of species of isolates.

Methods: A total of 353 burn patients admitted to three major ICUs of burn centers in Iran were evaluated between 2021 and 2023.

An Unusual High Prevalence of Cryptococcus (Naganishia) diffluens Colonization in Neonates Hospitalized in a Referral Neonatal Intensive Care Unit.

Background: Although the Candida species continue to be the most frequent colonizer of neonatal skin, a clear increase of colonization due to rare yeast-like fungi has been reported. In this study, we report an unusual high prevalence of Cryptococcus diffluens colonization in neonates admitted to the neonatal intensive care unit (NICU) over a 1-month period.

Methods: From January 2020 to June 2021, the study included all neonates who were admitted to the NICU of Bu Ali Sina Hospital at least 28 days old.

Clinic-mycological spectrum of infection in diabetic foot ulcers in a tertiary care hospital.

Background And Purpose: In diabetic foot ulcers, if fungal agents, such as species penetrate the cutaneous or depth of the ulcer, it can increase the wound severity and make it more difficult to heal.

Materials And Methods: A cross-sectional study was performed on 100 diabetic patients with a foot ulcer from December 2019 to November 2020 in northern Iran. Patient data and wound grades were recorded in a questionnaire.

antifungal activity of biosynthesized selenium nanoparticles using plant extracts and six comparators against clinical strains.

Background And Purpose: species are commonly resistant to many antifungal drugs. The limited therapeutic options available have led to a surge of research efforts aimed at discovering novel antifungal compounds in recent decades. This study aimed to assess the antifungal activity of plant-based biosynthesized selenium nanoparticles (Se NPs) and six comparators against a set of clinical strains.

Molecular Epidemiology and Antifungal Susceptibility Profile in Nakaseomyces glabrata Species Complex: A 5-Year Countrywide Study.

Background: The current study aimed to identify Iranian Nakaseomyces (Candida) glabrata complex species in the clinical isolates and determine their antifungal susceptibility profile.

Methods: In total, 320 N. glabrata clinical isolates were collected from patients hospitalized in different geographical regions of Iran.

The Gene in : Structure, Function, and Role in Azole Resistance: A Mini-Review.

Candidiasis is a common fungal infection caused by species, with being the most prevalent. Resistance to azole drugs, commonly used to treat infections, poses a significant challenge. Transcriptional activator candidate 1 () gene has emerged as a key player in regulating drug resistance in .

Molecular typing of a collection of Iranian clinical Trichophyton tonsurans isolates based on the non-transcribed spacer region of rDNA and antifungal susceptibility testing of the species.

Introduction: Wrestling, considered the national sport of Iran, has gained immense popularity among Iranians. Wrestlers frequently encounter skin conditions, with dermatophyte fungal infections, particularly tinea gladiatorum (TG), being a common issue. TG, caused by the Trichophyton genus, has emerged as a major health concern for wrestlers and other contact sport athletes worldwide.

Central nervous system Aspergillus quadrilineatus infection in a COVID-19 patient, a case report and literature review.

Background: Viral pneumonia such as COVID-19-associated aspergillosis could increase susceptibility to fungal super-infections in critically ill patients.

Methods: Here we report a pediatric case of Aspergillus quadrilineatus cerebral infection in a recently diagnosed COVID-19-positive patient underlying acute lymphocytic leukemia. Morphological, molecular methods, and sequencing were used to identify this emerging species.

Indoor environment assessment of special wards of educational hospitals for the detection of fungal contamination sources: A multi-center study (2019-2021).

Background And Purpose: The hospital environment was reported as a real habitat for different microorganisms, especially mold fungi. On the other hand, these opportunistic fungi were considered hospital-acquired mold infections in patients with weak immune status. Therefore, this multi-center study aimed to evaluate 23 hospitals in 18 provinces of Iran for fungal contamination sources.

Evaluation of colonization index, molecular identification, and antifungal susceptibility pattern of species isolated from critically ill pediatric patients: A single-center study in Iran.

Background And Purpose: Given the high mortality rate of invasive candidiasis in hospitalized pediatric patients, it is crucial to establish a predictive system to achieve early diagnosis and treatment of patients who are likely to benefit from early antifungal treatment. This study aimed to assess the colonization index, species distribution, and antifungal susceptibility pattern of strains isolated from pediatric patients with high colonization index (CI).

Materials And Methods: This study was carried out at the Children's Medical Center in Tehran-Iran.

A new record of isolated from paddy field soil in Iran: Phylogeny and antifungal susceptibility profiles.

Background And Purpose: species are ubiquitous environmental fungi, which are considered emerging agents that trigger disease in humans and animals. The present study aimed to determine strain isolated from paddy field soil samples using semi-selective media and evaluate its antifungal susceptibility profile.

Materials And Methods: Three paddy field soil samples were collected during an investigation for the isolation of species in Mazandaran province, Iran.

Azole-Containing Agar Plates and Antifungal Susceptibility Testing for the Detection of Azole-Resistant Species in Hospital Environmental Samples.

The indoor environment of hospitals should be considered as an important reservoir of azole resistant species. In this study, we evaluated azole-containing agar plates (ACAPs) and antifungal susceptibility testing (AFST) for the detection of azole-resistant species in hospital environmental samples. Between September 2021 and January 2022, environmental samples (108 instruments and 12 air) were collected from different wards of 4 educational hospitals in Mazandaran province, Iran.

The growth inhibitory and apoptotic effects of umbelliprenin in a mouse model of systemic candidiasis.

Aims: Umbelliprenin has shown promising biological activities, including immunoregulatory, anti-inflammatory, and anti-cancer effects. The present study investigated the growth inhibitory and apoptotic effects of umbelliprenin against Candida albicans in a BALB/c mice model of disseminated candidiasis.

Methods And Results: First, an antimicrobial assay via microdilution sensitivity test was performed.

Onychomycosis due to Fusarium species in different continents, literature review on diagnosis and treatment.

Fusarium species are an emerging cause of onychomycosis, and the number of cases has dramatically increased in recent decades worldwide. This review presents an overview of the onychomycosis cases caused by Fusarium species and diagnosis and treatment that have been reported in the literature. The most common causative agent of onychomycosis is F.