Fluorescence enhancement effect for the determination of adenosine 5'-monophosphate with 9-anthracene carboxylic acid-cetyl trimethyl ammonium bromide system.

A fluorimetric method based on fluorescence enhancement effect was developed for the determination of adenosine 5'-monophosphate (AMP) with 9-anthracene carboxylic acid (9-ANCA)-cetyl trimethyl ammonium bromide (CTAB) system. Fluorescence intensity of 9-ANCA was decreased by the addition of CTAB but addition of AMP again rose the intensity of 9-ANCA gradually. The observed fluorescence enhancement is attributed to the competitive binding reaction of 9-ANCA and adenosine to CTAB.