A new validated HPTLC method for quantitative determination of 1, 5-dicaffeoylquinic acid in Inula crithmoides roots.

1, 5-Dicaffeoylquinic acid (1, 5-DCQA), a potent HIV-1 integrase inhibitor, is currently undergoing an evaluation as a promising novel HIV therapeutic agent. This work aims at developing an accurate, rapid, repeatable and robust HPTLC method for the determination of 1, 5-DCQA in its natural sources. 1, 5-DCQA is the major component of the n-butanol fraction, the most biologically active hepatoprotective fraction, of Inula crithmoides roots extract.

Determination of lawsone in henna powders by high performance thin layer chromatography.

The lawsone content has been evaluated quantitatively in eight commercial henna powders and two collected henna leaves. The phenolic, chloroform-soluble fraction of the majority of the examined samples showed the presence of lawsone and two other pigments. Here we aimed to optimize high performance thin layer chromatography for the determination of lawsone.