This study revealed the presence of single nucleotide polymorphisms in the CXCR1 gene and their association with performance traits and mastitis incidence in Murrah buffalo. The targeted SNP rs211042414 (T > C) at the g.106216468 locus in partial exon 2 of the CXCR1 gene was genotyped using PCR amplification and restriction enzyme digestion by Alu I, Bsa I, Dde I, Ava I, Hind III, EcoRV, Hae III, and Hae II restriction enzymes.
View Article and Find Full Text PDFUnderstanding the genetic basis of growth and metabolic traits in sheep is crucial for improving production efficiency and sustainability. The current study aimed to estimate the genetic influences, both direct and maternal, on growth rate and Kleiber's ratio traits in Harnali sheep using pedigree data under Bayesian inference. The data pertained to 2404 animals spanned over 24 years (1998-2021).
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September 2024
Epigenetic mechanisms, such as DNA methylation, histone modifications and non-coding RNA molecules, play a critical role in gene expression and regulation in livestock species, influencing development, reproduction and disease resistance. DNA methylation patterns silence gene expression by blocking transcription factor binding, while histone modifications alter chromatin structure and affect DNA accessibility. Livestock-specific histone modifications contribute to gene expression and genome stability.
View Article and Find Full Text PDFThe present study explored the association between a point mutation (g.857G>A) in the IGF-1 gene and performance traits in Harnali Sheep. Genomic DNA was extracted from 110 Harnali sheep using an Automated Maxell RSC DNA/RNA purification system.
View Article and Find Full Text PDFThe study aimed to assess performance traits in Hardhenu cattle by analysing data from 445 animals born to 59 sires and 227 dams. The investigation focused on estimating (co)variance components and genetic parameters for reproduction and production traits in dairy cattle. Results from least-squares analysis indicated a significant effect (p < .
View Article and Find Full Text PDFThe present study was carried out to study the polymorphism in the GH gene and its association with various performance and body conformation traits, viz., birth weight (B-WT), weaning weight (W-WT), six-month body weight (6 M-WT), one-year body weight (Y-WT), annual greasy fleece weight (AGFW), body length (BL), body height (BH), heart girth (HG) and paunch girth (PG) in 138 Harnali sheep. PCR-RFLP was performed to identify polymorphism in the targeted region of the GH gene.
View Article and Find Full Text PDFSemen sexing is among one of the most remarkable inventions of the past few decades in the field of reproductive biotechnology. The urge to produce offspring of a desired sex has remained since traditional times. Researchers have tried many methods for accurate semen sexing, but only the flow cytometry method has proved to be effective for commercial utilization.
View Article and Find Full Text PDFSmall non-coding RNAs called microRNAs (miRNAs) control the expression of genes post-transcriptionally. Their correlation with commercial economic traits including milk, meat and egg production, as well as their effective role in animal productivity, fertility, embryo survival and disease resistance, make them significant in livestock research. The miRNAs exhibit distinct spatial and temporal expression patterns, offering insights into their functional roles within cells and tissues.
View Article and Find Full Text PDFThe proposed study was to determine if the silver nanoparticles can be used as potential antimicrobial agents and can replace the use of conventional antibiotics in semen without affecting the motility and fertility of semen. The silver nanoparticles prepared by chemical reduction method were confirmed by determination of the wavelength of surface plasmon resonance peak and further characterized using Zetasizer by determining their size, polydispersity index, and zeta potential. The nanoparticles were assessed for antibacterial activity and their concentration was optimized for use in semen extender for cryopreservation.
View Article and Find Full Text PDFThe present study investigated the effect of linseed supplementation on serum profiling and differential expression levels of IL-2 and TLR 2 genes involved in the immune system of lactating goats. The study was conducted on 15 lactating Beetal goats for 3 months, which were divided into three groups. Treatment group T was kept as a control group with no linseed supplementation, whereas treatment group T received ground linseed at the rate 10% of concentrate per day per animal, and treatment group T received 20% ground linseed.
View Article and Find Full Text PDFThis study aimed to explore the occurrence and risk factors associated with clinical mastitis within the Hardhenu cattle herd over a span of 14 years (2008-2021). A comprehensive analysis of 1515 lactation records was conducted to ascertain the incidence of clinical mastitis. The investigation determined an overall incidence rate of 26.
View Article and Find Full Text PDFLayered double hydroxides (LDHs) have gained vast importance as an electrocatalyst for water electrolysis to produce carbon-neutral and clean hydrogen energy. In this work, we demonstrated the fabrication of nano-flake-like NiMn LDH thin film electrodes onto porous membrane-like Ni-foam by using a simple and cost-effective electrodeposition method for oxygen evolution reaction (OER). Various NiMn LDH (where x = 0.
View Article and Find Full Text PDFThe aim of the present study is to carry out a risk analysis and Bayesian estimates of genetic effects on calf survival in Hardhenu cattle using data records of 2593 calves born to 102 sires and 790 dams over 25 years. The Bayesian analysis using Gibbs sampling was employed towards threshold animal models to estimate direct and maternal effects on animal survival of studied population. The results showed that mortality from birth to 3 months of age (S1), birth to 6 months of age (S2) and birth to 12 months of age (S3) was 10.
View Article and Find Full Text PDFThis study aimed to investigate the incidence and effect of non-genetic factors on reproductive disorders in the Hardhenu cattle population over a period of 11 years (2010-2021). The study included a total of 1340 cattle for calculating the incidence of reproductive disorders and analysed the occurrence of different reproductive disorders. In the present study, retention of placenta had highest frequency, accounting for approximately 18.
View Article and Find Full Text PDFThe present study investigated the presence of CXCR1 gene polymorphisms and their association with clinical mastitis, reproductive disorders and performance traits in Hardhenu cattle. Genotyping of the targeted SNP rs211042414 (C>T) at the g.106216468 loci of the CXCR1 gene was performed through PCR amplification and Bsa1 restriction enzyme digestion.
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May 2023
The study of growth curves in sheep is an effective approach to monitor the animal development for predicting the growth rate and improving overall flock performance. The purpose of the present work was to study the growth curve traits of Munjal sheep using different non-linear models and to estimate the genetic parameters of those traits for the possibility of inclusion of them under selection strategy. Total 2285 weight records at birth, 3, 6 and 12 months of age of 706 lambs born to 48 sires and 149 dams were collected from the registers maintained from 2004 to 2019.
View Article and Find Full Text PDFGenetic variability at the major histocompatibility complex (MHC) is important in any species due to significant role played by MHC for antigen presentation. DQA locus has not been studied for its genetic variability across sheep population in India. In the present study, MHC of sheep at DQA1 and DQA2 loci were evaluated across 17 Indian sheep breeds.
View Article and Find Full Text PDFThe aim of the present study was to evaluate the physiological and morphological parameters of pregnant does for early prediction of prenatal litter size. In total, 33 does were screened using ultrasonography and further categorized into three groups based on does bearing twins ( = 12), a single fetus ( = 12), or non-pregnant does ( = 9). The rectal temperature °F (RT) and respiration rate (RR) as physiological parameters, while abdominal girth in cm (AG) and udder circumference in cm (UC) as morphological parameters were recorded at different gestation times, i.
View Article and Find Full Text PDFGenetic polymorphism research in livestock species aims to assess genetic differences within and among breeds, primarily for conservation and development objectives. The aim of the present study was to determine the point mutation in the gene (g.855G>C and g.
View Article and Find Full Text PDFIn sheep, MHC variability is studied widely to explore disease association. The aim of the current study was to explore the genetic diversity of -DRB diversity across sheep breeds of India. Here, DRB1 locus was studied across 20 sheep breeds.
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September 2022
The purpose of the present study was to estimate the inbreeding coefficient and its effects on various growth traits in Beetal goat using pedigree records of 963 kids born to 38 sires and 287 dams over the period of 2004 to 2019. The inbreeding coefficients for each individual were obtained under animal model. The effects of inbreeding along with other fixed effects such as year of birth, sex of kid, type of birth, and dam's weight at kidding on growth traits viz.
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September 2022
The estimation of breeding values is prime concern for animal breeders in order to achieve desired genetic progress of farm animals. However, current methods for estimating BV involve simultaneous selection of animal model which are computationally intensive and time-consuming. The present attempt was made to predict breeding values of weaning trait under artificial neural networks (ANN), Bayesian technique (BT), and multiple regression (MR) methods.
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