Publications by authors named "Magnus Wolf"

The blue whale is an endangered and globally distributed species of baleen whale with multiple described subspecies, including the morphologically and genetically distinct pygmy blue whale. North Atlantic and North Pacific populations, however, are currently regarded as a single subspecies despite being separated by continental land masses and acoustic call differences. To determine the degree of isolation among the Northern Hemisphere populations, 14 North Pacific and 6 Western Australian blue whale nuclear and mitochondrial genomes were sequenced and analysed together with 11 publicly available North Atlantic blue whale genomes.

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Article Synopsis
  • The blue whale is the largest animal ever known, making its genome a key subject for studying longevity and cancer resistance.
  • Researchers created a detailed genome assembly of the blue whale using advanced sequencing methods and collaborated with databases like NCBI for annotation.
  • Findings revealed significant gene amplifications linked to the blue whale's size and genetic variations between Pacific and Atlantic populations, highlighting the genome's potential for future biological and conservation studies.
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The snake pipefish, (Linnaeus, 1758), is a northern Atlantic fish inhabiting open seagrass environments that recently expanded its distribution range. Here, we present a highly contiguous, near chromosome-scale genome of . The final assembly spans 1.

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  • Snakeflies, an ancient and small order of insects, have maintained their unique appearance for over 300 million years, yet they lack public interest and sufficient molecular research.
  • Researchers have published the first complete genome of the snakefly order, showing a total length of 669 Mbp and high-quality assembly metrics.
  • The study reveals significant genetic complexities and potential evolutionary events since the dinosaurs' extinction, providing a valuable resource for future research in understanding snakefly evolution and genetics.
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  • Ghrelin, a hormone linked to obesity and type 2 diabetes, undergoes modifications that affect its function, and this study explores its different forms (acylated and unacylated) in relation to body weight and insulin resistance.
  • The research involved 545 fasting participants and 245 individuals after an oral glucose tolerance test (oGTT), analyzing how these forms of ghrelin correlate with BMI and insulin sensitivity.
  • Findings revealed that higher BMI is associated with lower levels of both acylated and unacylated ghrelin, and the study suggests potential for targeting ghrelin modifications in treating obesity, despite overall lower ghrelin levels in individuals with higher BMI.
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Background: Baleen whales are a clade of gigantic and highly specialized marine mammals. Their genomes have been used to investigate their complex evolutionary history and to decipher the molecular mechanisms that allowed them to reach these dimensions. However, many unanswered questions remain, especially about the early radiation of rorquals and how cancer resistance interplays with their huge number of cells.

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Population-genomic studies can shed new light on the effect of past demographic processes on contemporary population structure. We reassessed phylogeographical patterns of a classic model species of postglacial recolonisation, the brown bear (Ursus arctos), using a range-wide resequencing dataset of 128 nuclear genomes. In sharp contrast to the erratic geographical distribution of mtDNA and Y-chromosomal haplotypes, autosomal and X-chromosomal multi-locus datasets indicate that brown bear population structure is largely explained by recent population connectivity.

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Despite increasing sequencing efforts, numerous fish families still lack a reference genome, which complicates genetic research. One such understudied family is the sand lances (Ammodytidae, literally: "sand burrower"), a globally distributed clade of over 30 fish species that tend to avoid tidal currents by burrowing into the sand. Here, we present the first annotated chromosome-level genome assembly of the great sand eel (Hyperoplus lanceolatus).

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It is generally recognized that large-scale whaling in the 19th and 20th century led to a substantial reduction of the size of many cetacean populations, particularly those of the baleen whales (Mysticeti). The impact of these operations on genomic diversity of one of the most hunted whales, the fin whale (Balaenoptera physalus), has remained largely unaddressed because of the paucity of adequate samples and the limitation of applicable techniques. Here, we have examined the effect of whaling on the North Atlantic fin whale based on genomes of 51 individuals from Icelandic waters, representing three temporally separated intervals, 1989, 2009 and 2018 and provide a reference genome for the species.

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Background: Genome sequencing of all known eukaryotes on Earth promises unprecedented advances in biological sciences and in biodiversity-related applied fields such as environmental management and natural product research. Advances in long-read DNA sequencing make it feasible to generate high-quality genomes for many non-genetic model species. However, long-read sequencing today relies on sizable quantities of high-quality, high molecular weight DNA, which is mostly obtained from fresh tissues.

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  • The common dragonet is one of three species found in the North Sea, exhibiting significant sexual dimorphism, especially in males, which have distinctive coloration and size, while females are less distinguishable.
  • A high-quality chromosome-level genome assembly was achieved using advanced sequencing techniques, resulting in an assembly length of 568.7 Mbp with a high level of completeness and over 19,000 annotated transcripts.
  • This genome assembly will serve as an important reference for future studies in population genetics and evolutionary biology of the species and its relatives.
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Recent advances in genome sequencing technologies have simplified the generation of genome data and reduced the costs for genome assemblies, even for complex genomes like those of vertebrates. More practically oriented genomic courses can prepare university students for the increasing importance of genomic data used in biological and medical research. Low-cost third-generation sequencing technology, along with publicly available data, can be used to teach students how to process genomic data, assemble full chromosome-level genomes, and publish the results in peer-reviewed journals, or preprint servers.

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Background: Currently available assays for the widely used marker for neuroendocrine tumors chromogranin A have a weak comparability, involve manual work, and require batch processing of the samples. In this study, we evaluated the automated chromogranin A KRYPTOR assay compared with the widely used DAKO ELISA.

Methods: 83 samples were measured with the DAKO ELISA (EDTA plasma) and the KRYPTOR assay (serum), since different sample materials are recommended.

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Context: Acylcarnitines are biomarkers of incomplete β-oxidation and mitochondrial lipid overload but indicate also high rates of mitochondrial fatty acid oxidation. It is unknown whether the production of acylcarnitines in primary human myotubes obtained from lean, metabolically healthy subjects reflects the fat oxidation in vivo.

Objective: Our objective was to quantify the acylcarnitine production in myotubes obtained from subjects with low and high fasting respiratory quotient (RQ).

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