Genomic and epigenomic active vitamin D responses in human colonic organoids.

Active vitamin D, 1α,25(OH)D, is a nuclear hormone with roles in colonic homeostasis and carcinogenesis; yet, mechanisms underlying these effects are incompletely understood. Human organoids are an ideal system to study genomic and epigenomic host-environment interactions. Here, we use human colonic organoids to measure 1α,25(OH)D responses on genome-wide gene expression and chromatin accessibility over time.