Ultra-Performance Liquid Chromatography and Mass Spectrometry Characterization, and Antioxidant, Protective, and Anti-Inflammatory Activity, of the Polyphenolic Fraction from .

is a valuable plant widely consumed worldwide and considered a rich source of polyphenols. This study examined the impact of the polyphenolic fraction isolated from basil (ObF) on human normal colon epithelial cells and human colorectal adenocarcinoma cells, evaluating its anti-inflammatory and protective activity against oxidative stress. The phytochemical characterization of the fraction was performed using ultra-performance liquid chromatography (UPLC) with a photodiode detector (DAD) and mass spectrometry (MS).

Antioxidant and Anti-Inflammatory Effects of Nettle Polyphenolic Extract: Impact on Human Colon Cells and Cytotoxicity Against Colorectal Adenocarcinoma.

L. is one of the most widely utilized medicinal plants commonly applied in the form of tea, juice, and dietary supplements. This study aimed to assess the effect of the ethanol-water extract (UdE) and polyphenolic fraction isolated from the extract (UdF) on normal human colon epithelial cells and to evaluate their protective activity against induced oxidative stress.

Optimizing the Extraction of the Polyphenolic Fraction from Defatted Strawberry Seeds for Tiliroside Isolation Using Accelerated Solvent Extraction Combined with a Box-Behnken Design.

Tiliroside is a natural polyphenolic compound with a wide range of biological activity, and defatted strawberry seeds are its rich source. The goal of this study was to optimize accelerated solvent extraction (ASE) conditions, including temperature, solvent composition, and the number of extraction cycles, using Box-Behnken design to maximize the yield of tiliroside. UPLC-DAD-MS was applied to investigate the polyphenolic composition of the extracts, and preparative liquid chromatography (pLC) was used for isolation.

Protective Effect of Extract against Oxidative Stress in Human Skin Fibroblasts.

is a species with well-established significance in folk medicine in many countries. It was utilized to support the treatment of arthritis, allergies, and urinary tract disorders; however, the substantial presence of antioxidants suggests that nettle extract could also have a positive impact on the skin. The objective of this study was to assess the impact of nettle extract on human skin fibroblasts subjected to oxidative stress.

Overexpression of Bacterial Beta-Ketothiolase Improves Flax ( L.) Retting and Changes the Fibre Properties.

Beta-ketothiolases are involved in the beta-oxidation of fatty acids and the metabolism of hormones, benzenoids, and hydroxybutyrate. The expression of bacterial beta-ketothiolase in flax ( L.) results in an increase in endogenous beta-ketothiolase mRNA levels and beta-hydroxybutyrate content.

Oligo-Not Only for Silencing: Overlooked Potential for Multidirectional Action in Plants.

Oligo technology is a low-cost and easy-to-implement method for direct manipulation of gene activity. The major advantage of this method is that gene expression can be changed without requiring stable transformation. Oligo technology is mainly used for animal cells.

Replacing cutting guides with an augmented reality-based navigation system: A feasibility study in the maxillofacial region.

Purpose: The work presents outcomes of simulated maxillofacial skeleton osteotomies supported with cutting guides or one of two AR-based intraoperative navigation systems.

Material And Methods: The series of osteotomies supported with a cutting guide, simple AR (sAR) or navigated AR (nAR) module were carried out on 15 (five per each method) skull models according to the virtual surgical plan (VSP). Each method was used to support 40 osteotomies involving the upper jaw and 40 involving the orbital region (16 osteotomies on each model).

Quantitative evaluation f stereoscopic augmented reality visualization using an optical see-through head-mounted display.

Purpose: The authors of the current study aimed to propose a calibration method for accurate augmented reality visualisation using an optical see-through head-mounted display, and to qualitatively evaluate visualisation accuracy for the application in computer assisted surgery.

Methods: An adaptation of stereo single-point active alignment method was proposed as the calibration procedure together with verification. Three tests were performed: display of points in 3D space, on the plane and on the surface of a skull phantom on inexperience users (5 participants) and experienced users (17 participants).

"Image to patient" equal-resolution surface registration supported by a surface scanner: analysis of algorithm efficiency for computer-aided surgery.

Purpose: The "image to patient" registration procedure is crucial for the accuracy of surgical instrument tracking relative to the medical image while computer-aided surgery. The main aim of this work was to create an equal-resolution surface registration algorithm (ERSR) and analyze its efficiency.

Methods: The ERSR algorithm provides two datasets with equal, high resolution and approximately corresponding points.

Celastrol and Resveratrol Modulate Genes Expression and Exert Anticancer Activity in Colon Cancer Cells and Cancer Stem-like Cells.

Metastatic colorectal cancer (CRC) remains a hard-to-cure neoplasm worldwide. Its curability declines with successive lines of treatment due to the development of various cancer resistance mechanisms and the presence of colorectal cancer stem cells (CSCs). Celastrol and resveratrol are very promising phytochemicals for colon cancer therapy, owing to their pleiotropic activity that enables them to interact with various biological targets.

The Antimicrobial Properties of Poplar and Aspen-Poplar Propolises and Their Active Components against Selected Microorganisms, including .

There is a noticeable interest in alternative therapies where the outcome is the eradication of the Gram-negative bacterium, (), for the purpose of treating many stomach diseases (chronic gastritis and peptic ulcers) and preventing stomach cancer. It is especially urgent because the mentioned pathogen infects over 50% of the world's population. Recent studies have shown the potential of natural products, such as medicinal plant and bee products, on the inhibition of growth.

The Technological Process of Obtaining New Linen Dressings Did Not Cause the Loss of Their Wound-Healing Properties.

Background: Linen dressings were invented a few years ago but are still being worked on.

Methods: The obtained fabrics from the traditional variety of flax (Nike), two transgenic types of flax (M50 and B14) and the combination of these two flax fibers (M50 + B14) were tested in direct contact in cell cultures. Cell viability tests were performed, and the proliferation potential of cells on Balb3T3 and NHEK cell lines was checked using the Sulforhodamine-B (SRB) test.

Wound coverage by the linen dressing accelerates ulcer healing.

Introduction: Chronic ulcers are the main cause of morbidity and mortality, and the incidence of chronic wounds is expected to increase given that people live longer and that there are civil diseases.

Aim: Much attention in the treatment of wounds concerns a dressing that involves wound cleansing, bacterial balance, exudate management and local tissue in a wound environment. These important elements of the evaluation led to the development of an interactive dressing based entirely on flax raw materials.

Augmented reality visualization for aiding biopsy procedure according to computed tomography based virtual plan.

Purpose: The purpose of this study was to develop and verify an intraoperative module for supporting navigated biopsy procedures using optical see-through head-mounted display (HMD).

Methods: Biopsy procedure including entry and endpoints of needle insertion was planned preoperatively having regard to the resection region segmentation and safety margin definition. Biopsy procedures were performed by two users using an intraoperative optical navigation module on a specially prepared brain phantom.

Linseed Silesia, Diverse Crops for Diverse Diets. New Solutions to Increase Dietary Lipids in Crop Species.

The aim of the work was to compare the new variety of oil flax (Silesia) with already cultivated varieties in terms of plant productivity, oil content, fatty acid composition and significant secondary metabolites. The analyzed linseed varieties are characterized by low (Linola), medium (Silesia) and high (Szafir) content of omega-3 fatty acids. Special attention was paid to the quality of the oil and the characteristics that determine its stability (reduction of susceptibility to oxidation).

Atherosclerosis Development and Aortic Contractility in Hypercholesterolemic Rabbits Supplemented with Two Different Flaxseed Varieties.

Alpha-linolenic acid (ALA) is widely regarded as the main beneficial component of flax for the prevention of cardiovascular disease. We evaluated the effect of the transgenic flaxseed W86-which is rich in ALA-on the lipid profile, atherosclerosis progression, and vascular reactivity in hypercholesterolemic rabbits compared to the parental cultivar Linola with a very low ALA content. Rabbits were fed a basal diet (control) or a basal diet supplemented with 1% cholesterol, 1% cholesterol and 10% flaxseed W86, or 1% cholesterol and 10% Linola flaxseed.

Impact of Plant Origin on Eurasian Propolis on Phenolic Profile and Classical Antioxidant Activity.

Propolis is a bee product with known medical properties, including antioxidant activity. The scope of the study is profiling 19 different Eurasian propolis samples (mostly from Russia and Kazakhstan, Kyrgyzstan, Poland, Ukraine, and Slovakia). Profiles of propolises were investigated by ultra-high-performance liquid chromatography-diode array detector-mass spectrometry (UPLC-DAD-MS).

Were our Ancestors Right in Using Flax Dressings? Research on the Properties of Flax Fibre and Its Usefulness in Wound Healing.

Background: Despite the wide range of medical dressings available commercially, there is still a search for better biomaterials for use in the treatment of especially difficult-to-heal wounds. For several years, attention has been paid to the use of substances, compounds, and even whole plants in medicine. Flax is a plant that has been used as a dressing for thousands of years.

Metabolism of the Cyanogenic Glucosides in Developing Flax: Metabolic Analysis, and Expression Pattern of Genes.

Cyanogenic glucosides (CG), the monoglycosides linamarin and lotaustralin, as well as the diglucosides linustatin and neolinustatin, have been identified in flax. The roles of CG and hydrogen cyanide (HCN), specifically the product of their breakdown, differ and are understood only to a certain extent. HCN is toxic to aerobic organisms as a respiratory inhibitor and to enzymes containing heavy metals.

3-Hydroxybutyrate Is Active Compound in Flax That Upregulates Genes Involved in DNA Methylation.

In mammalian cells, 3-hydroxybutyrate (3-HB) is not only an intermediate metabolite during the oxidation of fatty acids, but also an important signaling molecule. On the other hand, the information about the metabolism or function of this compound in plants is scarce. In our study, we show for the first time that this compound naturally occurs in flax.

Transgenerational Perpetuation of CHS Gene Expression and DNA Methylation Status Induced by Short Oligodeoxynucleotides in Flax ().

Over two decades ago, short oligodeoxynucleotides (ODNs) were proven to be an effective and rapid technique for analysis of gene function without interference in the plant genome. Our previous research has shown the successful regulation of chalcone synthase (CHS) gene expression in flax by ODN technology. The CHS gene encodes a pivotal enzyme in flavonoid biosynthesis.

Use of Natural Components Derived from Oil Seed Plants for Treatment of Inflammatory Skin Diseases.

The incidence of inflammatory skin diseases is increasing, so the search for relevant therapeutics is of major concern. Plants are rich in phytochemicals which can alleviate many symptoms. In this review, we concentrate on compounds found in the seeds of widely cultivated plants, regularly used for oil production.

Temporal biosynthesis of flavone constituents in flax growth stages.

Previous studies showed that chalcone synthase (chs) silencing in flax (Linum usitatisimum) induces a signal transduction cascade that leads to extensive modification of plant metabolism. Result presented in the current study, performed on field grown flax plants - (across the whole vegetation period) demonstrates that, in addition to its role in tannin and lignin biosynthesis, the chs gene also participates in the regulation of flavone biosynthesis during plant growth. Apigenin and luteolin glycosides constitute the flavones, the major group of flavonoids in flax.

Supporting fibula free flap harvest with augmented reality: A proof-of-concept study.

Objective: To analyze a novel navigation system utilizing augmented reality (AR) as a supporting method for fibula free flap (FFF) harvest and fabrication.

Methods: A total of 126 simulated osteotomies supported with a cutting guide or one of two AR-based intraoperative navigation modules-simple AR (sAR) or navigated AR (nAR)-were carried out on 18 identical models of the fibula (42 osteotomies per method). After fusing postoperative computed tomography scans of the operated fibulas with the virtual surgical plan based on preoperative images, the objective outcomes-angular deviations from the planned osteotomy trajectory ( ) and deviations of control points marked on the trajectory (mm)-were determined.

Supporting mandibular resection with intraoperative navigation utilizing augmented reality technology - A proof of concept study.

Objective: The aim of this study was to compare the accuracy of simulated mandibular osteotomies performed with cutting guides and two different intraoperative navigation systems based on simple (sAR) and navigated (nAR) augmented reality technology.

Material And Methods: A total of 126 osteotomies were performed on 21 identical mandible models according to a prespecified virtual surgery plan. The data from postoperative computed tomography (CT) images were fused with preoperative CT scans to objectively compare the outcomes, i.