Analytical and clinical performance characteristics of the Abbott RealTime MTB RIF/INH Resistance, an assay for the detection of rifampicin and isoniazid resistant Mycobacterium tuberculosis in pulmonary specimens.

Clinical management of drug-resistant tuberculosis patients continues to present significant challenges to global health. To tackle these challenges, the Abbott RealTime MTB RIF/INH Resistance assay was developed to accelerate the diagnosis of rifampicin and/or isoniazid resistant tuberculosis to within a day. This article summarizes the performance of the Abbott RealTime MTB RIF/INH Resistance assay; including reliability, analytical sensitivity, and clinical sensitivity/specificity as compared to Cepheid GeneXpert MTB/RIF version 1.

Optical nonlinearity and electric conductivity origin study on sucrose crystal by using IR, Raman, INS, NMR, and EPR spectroscopies.

The supposed importance of hydrogen bonds toward the origin of second harmonic generation (SHG) and electric conductivity in crystalline sucrose was investigated by IR (4000-10cm(-1)), INS (2000-10cm(-1), at 35K), polarized Raman (3600-50cm(-1)) spectra, and (1)H NMR second moment line records in the temperature range 450-80K. The temperature dependence of NIR (7000-5500cm(-1)) polarized spectra gave information about -CH2 motions complementary to NMR results concerning -CH2OH group rearrangements. The EPR spectra were applied to study the generation of radical ions by exposure to NIR radiation.

Electrical anharmonicity and vibronic couplings contributions to optical nonlinearity of N-benzyl-2-methyl-4-nitroaniline crystal studied by FT-IR, polarized FT-NIR, resonance Raman and UV-vis spectroscopy.

FT-IR and Raman spectra of the orthorhombic and monoclinic N-benzyl-2-methyl-4-nitroaniline (BNA) single crystals, powders, and BNA solutions were measured in the 15-4000 cm(-1) range, and complete assignments of bands to normal vibrations are proposed. The assignments have been reinforced by density functional theory (DFT) calculations. Polarized FT-NIR spectra of the orthorhombic (010) BNA plate were measured for the overtones and combinations analysis and for the mechanical and electrical anharmonicity estimation.

Helical superstructure and charged polarons contributions to optical nonlinearity of 2-methyl-4-nitroaniline crystals studied by resonance Raman, electron paramagnetic resonance, circular dichroism spectroscopies, and quantum chemical calculations.

The Raman excitation profiles of solid 2-methyl-4-nitroaniline (MNA) reveal several band enhancements by intermolecular and intramolecular charge transfer states. Calculated excited- and ground-state molecular geometries and excited state distortions qualitatively determined from Raman spectra indicate multiple vibrations leading to MNA dissociation. Also, overtones and combination tones can generate charged polarons, as detected by electron paramagnetic resonance after the exposure to 980 and 1550 nm laser diodes.

Dynamical disorder in 2-methyl-4-nitroaniline and its deuterated analogue crystals studied by Fourier transform infrared and nuclear magnetic resonance.

The Fourier transform infrared spectra of the thin layers of 2-methyl-4-nitroaniline (MNA) and its deuterated analog were recorded in the 500-4000 cm(-1) region in the 10-300 K temperature range. Activation energies of the -CH(3), -NH(2), and -NO(2) groups reorientations were estimated. The (1)H-NMR spin-lattice relaxation time, T(1), and the second moment of (1)H-NMR resonance line, M(2), measured in the 80-298 K temperature range, were used to determine the parameters of the -CH(3) group motion.

Low-temperature photoluminescence of p-nitroaniline and o-methyl-p-nitroaniline crystals.

Photoemission spectra of para-nitroaniline (p-NA) and ortho-methyl-para-nitroaniline (MNA) single crystals measured between 5 and 250K revealed that the MNA crystals emit phosphorescence and fluorescence in contrast with the p-NA crystals which have only fluorescence. It is assumed that the fluorescence of p-NA crystals at 5K originates from the doublet state of negatively charged polarons while the fluorescence of MNA crystals at 60K is due to trap states. The Stokes shift between absorption and emission onsets, measured at 5K, is much larger in the p-NA crystals than in the MNA and meta-nitroaniline (m-NA) crystals.