Publications by authors named "Magdalena Ripoll"
Article Synopsis
- - The study focused on optimizing the production and recovery of the anticancer drug Taxol (paclitaxel) from vascular stem cells of Taxus baccata, using in situ product recovery (ISPR) techniques with various resin beads and methyl jasmonate as an elicitor.
- - The optimal combination of resin beads increased the paclitaxel yield significantly, producing 234 ± 23 mg of paclitaxel per kg of fresh-weight cells, which was 13 times higher than the control group that didn't use resins.
- - Additionally, using ISPR lowered the levels of reactive oxygen species (ROS) in the cultivations, which is linked to better cell growth and product synthesis, leading to
Bacterial immobilization is regarded as an enabling technology to improve the stability and reusability of biocatalysts. Natural polymers are often used as immobilization matrices but present certain drawbacks, such as biocatalyst leakage and loss of physical integrity upon utilization in bioprocesses. Herein, we prepared a hybrid polymeric matrix that included silica nanoparticles for the unprecedented immobilization of the industrially relevant (Gfr).
View Article and Find Full Text PDFDifferent from other aerobic microorganisms that oxidise carbon sources to water and carbon dioxide, Gluconobacter catalyses the incomplete oxidation of various substrates with regio- and stereoselectivity. This ability, as well as its capacity to release the resulting products into the reaction media, place Gluconobacter as a privileged member of a non-model microorganism class that may boost industrial biotechnology. Knowledge of new technologies applied to Gluconobacter has been piling up in recent years.
View Article and Find Full Text PDFArticle Synopsis
- Several immobilization strategies for Gluconobacter oxydans NBRC 14819 (Gox) were tested for converting crude glycerol to dihydroxyacetone (DHA), using materials like agar, agarose, and polyacrylamide.
- Agar-immobilized Gox proved to be the most effective, achieving 50 g/L glycerol conversion to DHA solely in water and allowing for reutilization over eight cycles, producing significantly more DHA than free cell batches.
- The study highlights the efficiency of immobilized biocatalysts, demonstrating high productivity in a water-based environment, which could reduce downstream processing costs.
Transaminases are a class of enzymes with promising applications for the preparation and resolution of a vast diversity of valued amines. Their poor operational stability has fueled many investigations on its stabilization due to their biotechnological relevance. In this work, we screened the stabilization of the tetrameric ω-transaminase from Pseudomonas fluorescens (PfωTA) through both carrier-bound and carrier-free immobilization techniques.
View Article and Find Full Text PDFIn the present work, glycerol biotransformation using Gluconobacter strains was studied with a process intensification perspective that facilitated the development of a cleaner and more efficient technology from those previously reported. Starting from the industrial by-product, crude glycerol, resting cells of Gluconobacter frateurii and Gluconobacter oxydans were able to convert glycerol under batch reactor conditions in water with no other additive but for the substrate. The study of strains, biomass:solution ratio, pH, growth stage, and simplification of media composition in crude glycerol bioconversions facilitated productivities of glyceric acid of 0.
View Article and Find Full Text PDF