Improved RAPD Method for Fingerprinting.

Recently, methods based on the analysis of arbitrarily amplified target sites of genome microorganisms have been extensively applied in microbiological studies, and especially in epidemiological studies. The range of their application is limited by problems with discrimination and reproducibility resulting from a lack of standardized and reliable methods of optimization. The aim of this study was to obtain optimal parameters of the Random Amplified Polymorphic DNA (RAPD) reaction by using an orthogonal array as per the Taguchi and Wu protocol, modified by Cobb and Clark for isolates.

Molecular Investigation of the Fatal Bloodstream Infection Case following Gastrectomy.

represents a closely related cryptic genospecies of complex-misidentified in routine diagnostic assays. This is emerging in settings where central venous catheters, invasive medical interventions, and echinocandin treatments are most likely to be used. A 59-year-old, non-neutropenic male patient, was admitted to an intensive care unit (ICU) due to respiratory distress syndrome, following a partial gastrectomy.

Immunoreactivity of Polish Lyme Disease Patient Sera to Specific Antigens-Part 1.

The diverse clinical picture and the non-specificity of symptoms in Lyme disease (LD) require the implementation of effective diagnostics, which should take into account the heterogeneity of antigens. According to available guidelines, laboratories should use a two-tier serological diagnosis based on the enzyme-linked immunosorbent (ELISA) screening test and confirmation of the immunoblot (IB). The aim of the study was to investigate the immunoreactivity of LD patient sera to antigens and to attempt to identify the genospecies responsible for LD using an ELISA-IB assay combination.

The effect of fennel essential oil and -anethole on antibacterial activity of mupirocin against isolated from asymptomatic carriers.

Introduction: constitutes the most pathogenic species within the staphylococcal genus. Humans remain the major reservoirs of this pathogen which colonizes mostly anterior nares of healthy individuals.

Aim: To investigate the effect of fennel essential oil (FEO) and -anethole (A) on antibacterial activity of mupirocin (MUP) against strains isolated from asymptomatic carriers.

The effect of fennel essential oil in combination with antibiotics on Staphylococcus aureus strains isolated from carriers.

An increase in the number of staphylococcal infections and carriers among medical staff has forced us to seek more and more effective antibacterial agents. Bacteria from the Staphylococcus genus possessing different mechanisms of resistance are the cause of nosocomial infections. The objective of our investigations was susceptibility of S.

[Anaerobic bacteria of periodontal pockets in course of periodontitis and premature labor].

The aim of the study is was to evaluate anaerobic periodontal mikrobes in 120 postpartum mothers. Group I consisted of mothers with a birth weight of less than 2,500g and gestational age < 37 weeks. Group II included mothers with normal birth weight infants.