Fast assessment of planar chromatographic layers quality using pulse thermovision method.

The main goal of this paper is to demonstrate capability of pulse thermovision (thermal-wave) methodology for sensitive detection of photothermal non-uniformities within light scattering and semi-transparent planar stationary phases. Successful visualization of stationary phases defects required signal processing protocols based on wavelet filtration, correlation analysis and k-means 3D segmentation. Such post-processing data handling approach allows extremely sensitive detection of thickness and structural changes within commercially available planar chromatographic layers.

Low-parachor solvents extraction and thermostated micro-thin-layer chromatography separation for fast screening and classification of spirulina from pharmaceutical formulations and food samples.

The goal of this paper is to demonstrate the separation and detection capability of eco-friendly micro-TLC technique for the classification of spirulina and selected herbs from pharmaceutical and food products. Target compounds were extracted using relatively low-parachor liquids. A number of the spirulina samples which originated from pharmaceutical formulations and food products, were isolated using a simple one step extraction with small volume of methanol, acetone or tetrahydrofuran.

Temperature-controlled micro-TLC: a versatile green chemistry and fast analytical tool for separation and preliminary screening of steroids fraction from biological and environmental samples.

This paper is a continuation of our previous research focusing on development of micro-TLC methodology under temperature-controlled conditions. The main goal of present paper is to demonstrate separation and detection capability of micro-TLC technique involving simple analytical protocols without multi-steps sample pre-purification. One of the advantages of planar chromatography over its column counterpart is that each TLC run can be performed using non-previously used stationary phase.

Application of micro-thin-layer chromatography as a simple fractionation tool for fast screening of raw extracts derived from complex biological, pharmaceutical and environmental samples.

The main goal of present paper is to demonstrate the separation and detection capability of micro-TLC technique involving simple one step liquid extraction protocols of complex materials without multi-steps sample pre-purification. In the present studies target components (cyanobacteria pigments, lipids and fullerenes) were isolated from heavy loading complex matrices including spirulina dried cells, birds' feathers and fatty oils as well as soot samples derived from biomass fuel and fossils-fired home heating systems. In each case isocratic separation protocol involving less that 1 mL of one component or binary mixture mobile phases can be completed within time of 5-8 min.

Optimization of a solid-phase extraction protocol for fractionation of selected steroids using retention data from micro thin-layer chromatography.

In this paper a simple protocol is described for estimating of solid-phase extraction (SPE) elution volumes of steroids based on retention data generated from micro-planar chromatography. Particularly, the retention of selected steroids, including estrogens and progestagens, was studied on wettable with water octadecylsilica HPTLC plates and mobile phases composed of methanol:water mixtures ranging from 20 to 100% (v/v). It was found that TLC retention data can be linearized by plotting R(M) values of steroids against a reciprocal form of the organic modifier molar fraction (1/X(s)).

Evaluation of the water and organic liquids extraction efficiency of Spirulina maxima dyes using thermostated micro thin-layer chromatography.

Thermostated micro thin-layer chromatography was applied for separation and quantification studies of Spirulina maxima dyes isolated from pharmaceutical formulation by a simple one-step liquid extraction. The isolation process was performed using a number of liquids, including water; 10 mM water solutions of native alpha-, beta-, and gamma-cyclodextrin and their hydroxypropyl derivatives; and a number of common organic liquids characterized by different polarity, namely, methanol, ethanol, 1-propanol, 2-propanol, acetonitrile, acetone, tetrahydrofuran, dichloromethane, toluene, and n-hexane. Chromatographic studies were performed on RP18W plates working inside a small thermostated horizontal chamber allowing a development distance of 45 mm.

Application of temperature-controlled micro planar chromatography for separation and quantification of testosterone and its derivatives.

Temperature-controlled micro thin-layer chromatography (TLC) was applied for separation and quantification studies of testosterone and its derivatives including methyltestosterone, testosterone propionate, isobutyrate, phenylpropionate, isocaproate, enanthate and caprate. Chromatographic studies were performed on silica-, octadecylsilica- and aluminum-coated plates working inside a small thermostated horizontal chamber unit allowing one-dimensional and two-dimensional developing modes with an elution distance of 45 mm. Retention properties of steroids were investigated across a whole range of binary mixtures such as methanol/water, acetonitrile/water, methanol/dichloromethane and acetone/hexane (0-100% v/v).