Amyloid Formation of Stefin B Protein Studied by Infrared Spectroscopy.

Background: Stefin B, an established model protein for studying the stability and mechanism of protein folding, was used for monitoring protein aggregation and formation of amyloid structure by infrared spectroscopy.

Methods: The analyses of the integral intensities of the low frequency part of the Amide I band, which is directly connected to the appearance of the cross-β structure reveals the temperature but not pH dependence of stefin B structure.

Results: We show that pH value has significant role in the monomer stability of stefin B.