Immunochemotherapy for cryptosporidiosis in immunosuppressed mouse model.

Immunochemotherapy as a dual regimen (Nitazoxanide NTZ and Interferon gamma INF-gamma) and a triple one (NTZ, INF-gamma & Paromomycin PRM), administered to immunosuppressed Cryptosporidium infected mice for 10 days (4th-13th day post-infection) was evaluated during and after treatment by determination of parasite count in ileum, associated histopathological changes, oocyst count in Kinyoun's acid fast stained faecal smears, percent reduction in oocyst excretion and cure rate. Both regimens induced nearby efficacy (P > 0.05) with significant reduction in parasite count in the ileum on 7th (P < 0.

Cryptosporidiosis among immunocompromised patients in Saudi Arabia.

A total of (408) immunocompromised Saudi patients (<2 - >60 years) checked for Cryptosporidium infection showed 69.7% and 64.2% infection rates by Kinyoun's acid fast staining for oocysts and a monoclonal ELISA kit for C.

Serological survey on toxoplasmosis among slaughtered sheep and goats in Tabouk, Saudi Arabia.

Sera from 397 sheep and 290 goats slaughtered at Tabouk Official Abattoir were examined for anti-Toxoplasma IgG by LAT, IHAT & IFAT. The results showed infection rates 23.4, 41.

Effect of some immunomodulators on the host-parasite system in experimental Hymenolepiasis nana.

Mice experimentally infected with H. nana and injected with immunosuppressant {cyclophosphamide (Cp) and lead nitrate (Ln)} showed significant increase in infection intensity, significant decrease in intestinal mast cell count, dissemination of larvae to the liver, toxic hepatitis and absence of specific serum IgG. Cyclophosphamide caused morphological abnormallities in adult worms, prolongation of patent period and more severe villous changes.

Fascioliasis among local and imported sheep in Saudi Arabia: parasitological and serological diagnosis.

Eight-hundred-twelve local and imported sheep, slaughtered at Riyadh abattoir, were subjected for parasitological diagnosis of fascioliasis by detection of eggs in the stool and worms in the liver and for serological diagnosis by detection of circulating anti-Fasciola IgG (CAFIgG) and circulating Fasciola antigens (CFAgs) using the indirect ELISA and the double antibody sandwich ELISA respectively. Detection of eggs revealed 13.5% infection rate compared with 21.

Detection of DNA of W. bancrofti in blood samples by QC-PCR-ELISA-based.

An internal control was used in a polymerase chain reaction PCR-ELISA-based technique to detect the DNA repeat of the filarial parasite W. bancrofti. The sensitivity of the test could detect as low as one single microfilaria added to 200 ul of blood.