Identification of two new intimin types in atypical enteropathogenic Escherichia coli.

Stool specimens of patients with diarrhea or other gastrointestinal alterations who were admitted to Xeral-Calde Hospital (Lugo, Spain) were analyzed for the prevalence of typical and atypical enteropathogenic Escherichia coli (EPEC). Atypical EPEC strains (eae+ bfp-) were detected in 105 (5.2%) of 2015 patients, whereas typical EPEC strains (eae+ bfp+) were identified in only five (0.

New roles for key residues in helices H1 and H2 of the Escherichia coli H-NS N-terminal domain: H-NS dimer stabilization and Hha binding.

Bacterial nucleoid-associated proteins H-NS and Hha modulate gene expression in response to environmental factors. The N-terminal domain of H-NS is involved in homomeric and heteromeric protein-protein interactions. Homomeric interaction leads to the formation of dimers and higher oligomers.

Aggregation profile characterisation in dielectrophoretic structures using bacteria and submicron latex particles.

A novel quantitative characterisation method for the measurement of anomalous low frequency aggregation processes on dielectrophoresis electrodes has been developed. Experimental evidence is provided for the relationship between the aggregation effect and AC electro-osmotical fluid motion theory. The aggregation profile dependence for E.

Osmoregulation of the HtrA (DegP) protease of Escherichia coli: an Hha-H-NS complex represses HtrA expression at low osmolarity.

The HtrA protein of Escherichia coli is a heat-shock inducible periplasmic protease, essential for bacterial survival at high temperatures. Expression of htrA gene depends on the alternative factor sigmaE and on the two-component regulatory system Cpx. These regulators systems respond, among others factors, to overproduction of misfolded proteins in the periplasm or to high level synthesis of various extracytoplasmic proteins.

Regulation of finP transcription by DNA adenine methylation in the virulence plasmid of Salmonella enterica.

DNA adenine methylase (Dam(-)) mutants of Salmonella enterica serovar Typhimurium contain reduced levels of FinP RNA encoded on the virulence plasmid. Dam methylation appears to regulate finP transcription, rather than FinP RNA stability or turnover. The finP promoter includes canonical -10 and -35 modules and depends on the sigma(70) factor.

Functional replacement of the oligomerization domain of H-NS by the Hha protein of Escherichia coli.

Members of the H-NS family of proteins play a relevant role as modulators of gene expression in gram-negative bacteria. Interaction of these proteins with members of the Hha/YmoA family of proteins has been previously reported. It has been hypothesized that the latter proteins are functionally equivalent to the N-terminal domain of H-NS-like proteins.

[Dental extractions in patients taking anticoagulants: is alteration of the anticoagulant regime necessary?].

A major concern in the management of patients under anticoagulants is the potential for excessive bleeding after dental procedures. Recommendations for the administration of oral anticoagulants in conjunction with oral surgery range from complete withdrawal of anticoagulants to the maintenance of an unchanged therapy. Rising evidences show that the alteration of anticoagulation is not necessary for patients with INR of 4 or less previous to tooth extractions.

Temperature-dependent conjugative transfer of R27: role of chromosome- and plasmid-encoded Hha and H-NS proteins.

IncHI plasmids encode multiple-antibiotic resistance in Salmonella enterica serovar Typhi. These plasmids have been considered to play a relevant role in the persistence and reemergence of this microorganism. The IncHI1 plasmid R27, which can be considered the prototype of IncHI plasmids, is thermosensitive for transfer.

YdgT, the Hha paralogue in Escherichia coli, forms heteromeric complexes with H-NS and StpA.

In enteric bacteria, proteins of the Hha/YmoA family play a role in the regulation of gene expression in response to environmental factors. Interaction of both Hha and YmoA with H-NS has been reported, and an Hha/H-NS complex has been shown to modulate expression in Escherichia coli of the haemolysin operon of plasmid pHly152. In addition to the hns gene, the chromosome of E.

Chronic Sialadenitis revealing hepatitis C: a case report.

One 53-year-old male was referred with a history of sensitive peripheral neuropathy and Raynaud disease leading to suspect a Sjögren syndrome (SS). Labial salivary gland biopsy shown the classical features of chronic lymphocytic sialadenitis. As clinical and immunologic tests were positive, we conclude to a chronic lymphocytic sialadenitis simulating SS.

In vivo increase of solubility of overexpressed Hha protein by tandem expression with interacting protein H-NS.

Gene cloning in appropriate vectors followed by protein overexpression in Escherichia coli is the common means for protein purification. This approach has many advantages but also some drawbacks; one of these is that many proteins fail to achieve a soluble conformation when overexpressed in E. coli.

Congenital anomalies of the tracheobronchial tree, lung, and mediastinum: embryology, radiology, and pathology.

Congenital anomalies of the chest are an important cause of morbidity in infants, children, and even adults. The evaluation of affected patients frequently requires multiple imaging modalities to diagnose the anomaly and plan surgical correction. The authors analyze and illustrate practical aspects of certain common and uncommon congenital anomalies affecting the tracheobronchial tree, lung, and mediastinum, with emphasis on radiologic manifestations.

[The use of water to seal facial mask for non invasive ventilation reduces the incidence of pressure ulcers].

Unlabelled: To demostrate that the use of water to seal facial mask for non invasive ventilation (NIV) reduces the incidence of facial pressure ulcers.

Methods: prospective study including 47 pacients who (according to their responsable physician) needs NIV. Intersurgical facial mask and Puritann Bennet 7200 ventilator are used.

Heterotopic gastric mucosa involving the gallbladder and biliary tree.

A case of heterotopic gastric mucosa in the common bile duct, cystic duct and gallbladder is reported in a 3-year-old girl with abdominal pain and jaundice. Abdominal US and CT showed dilatation of the biliary tree and a well-defined mass in the common bile duct that narrowed its lumen. The gallbladder was contracted in both examinations.

Primary mesenteric tumor with phenotypical features of gastrointestinal stromal tumors.

We report a case of a primary mesenteric tumor that was discovered in a 71-year-old man. Abdominal US accurately visualized the internal structure of the tumor but failed to determine the site of origin. Computed tomography suggested the mesenteric origin of the tumor before resection.

Temperature- and H-NS-dependent regulation of a plasmid-encoded virulence operon expressing Escherichia coli hemolysin.

Proteins H-NS and Hha form a nucleoprotein complex that modulates expression of the thermoregulated hly operon of Escherichia coli. We have been able to identify two H-NS binding sites in the hly regulatory region. One of them partially overlaps the promoter region (site II), and the other is located about 2 kbp upstream (site I).

Spontaneous pneumothorax during pregnancy.

Spontaneous pneumothorax complicating pregnancy is rare. Only 41 cases have been previously published. We describe a case of spontaneous pneumothorax successfully treated with tube thoracostomy during the 38th week of pregnancy.

New approaches to the management of peripheral vertigo: efficacy and safety of two calcium antagonists in a 12-week, multinational, double-blind study.

Objective: To evaluate the efficacy and safety profile of one 30-mg nimodipine oral tablet taken three times per day (one tablet with breakfast, one with lunch, and one with dinner) or one 150-mg cinnarizine verum oral capsule taken once each day with dinner for 12 weeks.

Study Design: Comparative in a double-blind, multinational pilot study.

Setting: Tertiary referral center.

Role of the Hha/YmoA family of proteins in the thermoregulation of the expression of virulence factors.

Virulent bacteria are able to sense temperature changes and respond by modifying the expression of--among others--genes that code for virulence factors. The chromatin-associated protein H-NS has been shown to play a role in the thermomodulation of virulence factor expression. In addition to H-NS, proteins of the Hha/YmoA family have also been identified in different enterobacteria as participating in the thermoregulation of some virulence factors.

Evidence for direct protein-protein interaction between members of the enterobacterial Hha/YmoA and H-NS families of proteins.

Escherichia coli nucleoid-associated H-NS protein interacts with the Hha protein, a member of a new family of global modulators that also includes the YmoA protein from Yersinia enterocolitica. This interaction has been found to be involved in the regulation of the expression of the toxin alpha-hemolysin. In this study, we further characterize the interaction between H-NS and Hha.

[An evaluation of an outbreak of American cutaneous leishmaniasis in a rural community in Bolivar State, Venezuela].

An epidemiological survey based on the Montenegro intradermal reaction (IDR) was carried out in San José de Hacha, south of the Bolivar State, Venezuela, on the occasion of an outbreak of American tegumentary leishmaniasis (LTA) in that locality. The IDR was applied to 121 of the 184 local inhabitants (65.8%), with a 33.

Expression of the hemolysin operon in Escherichia coli is modulated by a nucleoid-protein complex that includes the proteins Hha and H-NS.

The Escherichia coli protein Hha is a temperature- and osmolarity-dependent modulator of the expression of the hemolysin operon. The Hha protein was purified and its DNA-binding properties analyzed. Hha binds in a non-specific manner throughout the upstream regulatory region of the hemolysin operon in the recombinant hemolytic plasmid pANN202-312.