The immunopathological landscape of human pre-TCRα deficiency: From rare to common variants.

We describe humans with rare biallelic loss-of-function variants impairing pre-α T cell receptor (pre-TCRα) expression. Low circulating naive αβ T cell counts at birth persisted over time, with normal memory αβ and high γδ T cell counts. Their TCRα repertoire was biased, which suggests that noncanonical thymic differentiation pathways can rescue αβ T cell development.

Determination of trace perchlorate in river water by ion chromatography with online matrix removal and sample concentration.

This paper proposes a simple ion chromatographic approach to determine trace amounts of perchlorate in river water samples. Determination of the trace perchlorate by ion chromatography typically faces two challenges: interference by matrix ions such as chloride, nitrate, and sulfate in the samples and insufficient detection sensitivity. In the present study, online pretreatment of the samples with an OnGuard II Ba/Ag/H disposable sample pretreatment cartridge prevented the sulfate peak tailing from overlapping with the perchlorate peak on the chromatogram.

Change in membrane potential induced by streptolysin O, a pore-forming toxin: flow cytometric analysis using a voltage-sensitive fluorescent probe and rat thymic lymphocytes.

Streptolysin O (SLO) is a bacterial pore-forming toxin that is employed to permeabilize cell membranes in some biological experiments. SLO forms various types of pores with different shapes, increasing membrane ion permeability and subsequently inducing changes in membrane potential. To characterize the pores formed by SLO, the changes in membrane potential induced by SLO in rat lymphocytes were considered using flow cytometry with a voltage-sensitive fluorescent probe, bis-(1,3-dibutylbarbituric acid)trimethine oxonol (Oxonol).