The Arabidopsis apyrase AtAPY1 is localized in the Golgi instead of the extracellular space.

Background: The two highly similar Arabidopsis apyrases AtAPY1 and AtAPY2 were previously shown to be involved in plant growth and development, evidently by regulating extracellular ATP signals. The subcellular localization of AtAPY1 was investigated to corroborate an extracellular function.

Results: Transgenic Arabidopsis lines expressing AtAPY1 fused to the SNAP-(O(6)-alkylguanine-DNA alkyltransferase)-tag were used for indirect immunofluorescence and AtAPY1 was detected in punctate structures within the cell.