Publications by authors named "Madison E Smither"
Article Synopsis
- * Researchers have created new methods (RNA-Seq and PACBio sequencing) to pinpoint specific L1 loci that actually produce unique L1-related RNA, separating them from related sequences within genes.
- * Over 99% of L1-related RNA does not come from the L1 promoter; instead, it consists of fragments integrated into other cellular RNAs, with only a few active L1 loci being responsible for genuine L1 transcripts that vary by tissue type.
Background: Approximately 17 % of the human genome is comprised of the Long INterspersed Element-1 (LINE-1 or L1) retrotransposon, the only currently active autonomous family of retroelements. Though L1 elements have helped to shape mammalian genome evolution over millions of years, L1 activity can also be mutagenic and result in human disease. L1 expression has the potential to contribute to genomic instability via retrotransposition and DNA double-strand breaks (DSBs).
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