Searching for Gravitational Waves from Cosmological Phase Transitions with the NANOGrav 12.5-Year Dataset.

We search for a first-order phase transition gravitational wave signal in 45 pulsars from the NANOGrav 12.5-year dataset. We find that the data can be modeled in terms of a strong first order phase transition taking place at temperatures below the electroweak scale.

Extensive Structural Remodeling of the Axonal Arbors of Parvalbumin Basket Cells during Development in Mouse Neocortex.

Parvalbumin-containing (PV) basket cells are specialized cortical interneurons that regulate the activity of local neuronal circuits with high temporal precision and reliability. To understand how the PV interneuron connectivity underlying these functional properties is established during development, we used array tomography to map pairs of synaptically connected PV interneurons and postsynaptic neurons from the neocortex of mice of both sexes. We focused on the axon-myelin unit of the PV interneuron and quantified the number of synapses onto the postsynaptic neuron, length of connecting axonal paths, and their myelination at different time points between 2 weeks and 7 months of age.

Gephyrin-Lacking PV Synapses on Neocortical Pyramidal Neurons.

Gephyrin has long been thought of as a master regulator for inhibitory synapses, acting as a scaffold to organize γ-aminobutyric acid type A receptors (GABARs) at the post-synaptic density. Accordingly, gephyrin immunostaining has been used as an indicator of inhibitory synapses; despite this, the pan-synaptic localization of gephyrin to specific classes of inhibitory synapses has not been demonstrated. Genetically encoded fibronectin intrabodies generated with mRNA display (FingRs) against gephyrin (Gephyrin.

Challenges in Establishing a Coronavirus Disease 2019 (COVID-19) Convalescent Plasma Donation Program in a Multicultural Environment.

Context.—: In the face of the coronavirus disease 2019 (COVID-19) pandemic response, it was worthwhile to test the safety and efficacy of COVID-19 convalescent plasma (CCP) transfusion.

Objective.

Conduction Velocity Along the Local Axons of Parvalbumin Interneurons Correlates With the Degree of Axonal Myelination.

Parvalbumin-containing (PV+) basket cells in mammalian neocortex are fast-spiking interneurons that regulate the activity of local neuronal circuits in multiple ways. Even though PV+ basket cells are locally projecting interneurons, their axons are myelinated. Can this myelination contribute in any significant way to the speed of action potential propagation along such short axons? We used dual whole cell recordings of synaptically connected PV+ interneurons and their postsynaptic target in acutely prepared neocortical slices from adult mice to measure the amplitude and latency of single presynaptic action potential-evoked inhibitory postsynaptic currents.

Bacteriophages against Vibrio coralliilyticus and Vibrio tubiashii: Isolation, Characterization, and Remediation of Larval Oyster Mortalities.

and are pathogens responsible for high larval oyster mortality rates in shellfish hatcheries. Bacteriophage therapy was evaluated to determine its potential to remediate these mortalities. Sixteen phages against and were isolated and characterized from Hawaiian seawater.

Disruption of circadian timing increases synaptic inhibition and reduces cholinergic responsiveness in the dentate gyrus.

We investigated synaptic mechanisms in the hippocampus that could explain how loss of circadian timing leads to impairments in spatial and recognition memory. Experiments were performed in hippocampal slices from Siberian hamsters (Phodopus sungorus) because, unlike mice and rats, their circadian rhythms are easily eliminated without modifications to their genome and without surgical manipulations, thereby leaving neuronal circuits intact. Recordings of excitatory postsynaptic field potentials and population spikes in area CA1 and dentate gyrus granule cells revealed no effect of circadian arrhythmia on basic functions of synaptic circuitry, including long-term potentiation.

Spike frequency-dependent inhibition and excitation of neural activity by high-frequency ultrasound.

Ultrasound can modulate action potential firing in vivo and in vitro, but the mechanistic basis of this phenomenon is not well understood. To address this problem, we used patch-clamp recording to quantify the effects of focused, high-frequency (43 MHz) ultrasound on evoked action potential firing in CA1 pyramidal neurons in acute rodent hippocampal brain slices. We find that ultrasound can either inhibit or potentiate firing in a spike frequency-dependent manner: at low (near-threshold) input currents and low firing frequencies, ultrasound inhibits firing, while at higher input currents and higher firing frequencies, ultrasound potentiates firing.

Persistent Astrometric Deflections from Gravitational-Wave Memory.

Gravitational waves (GWs) produce small distortions in the observable distribution of stars in the sky. We describe the characteristic pattern of astrometric deflections created by a specific gravitational waveform called a burst with memory. Memory is a permanent, residual distortion of space left in the wake of GWs.

Improved theoretical calculations for electron-impact ionization of DNA analogue molecules.

Ionizing interactions between charged particles and molecules of biological relevance have attracted considerable interest in the last decade due to its importance in medical radiation therapy. We have previously calculated triply differential cross sections for five biomolecules in collaboration with experimental groups. We used the molecular 3-body distorted wave approximation for these calculations.

Triple differential cross-section measurements for electron-impact ionization of methane from a coplanar geometry to the perpendicular plane.

A new study of electron-impact single ionization of the HOMO 1t state of CH has been conducted at incident electron energies 20 eV and 40 eV above the ionization energy of the state. Triple differential cross sections were measured from a coplanar symmetric geometry, where scattered and ionized electrons were detected at equal angles, through to the perpendicular geometry where the outgoing electrons emerged orthogonal to the incident electron beam. At the lower energy, the electrons were detected with equal energies of 10 eV, whereas at the higher energy, data were obtained for equal energies of 20 eV and for unequal energies of 5 eV and 35 eV.

A dynamical (e,2e) investigation into the ionization of the outermost orbitals of R-carvone.

We report an experimental and theoretical investigation into the dynamics of electron-impact ionization of R-carvone. Experimental triple differential cross sections are obtained in asymmetric coplanar kinematic conditions for the ionization of the unresolved combination of the three outermost molecular orbitals (41a-39a) of R-carvone. These cross sections are compared with theoretical cross sections calculated within a molecular 3-body distorted wave (M3DW) framework employing either a proper orientation average or orbital average to account for the random orientation of the molecule probed in the experiment.

Multifaceted Changes in Synaptic Composition and Astrocytic Involvement in a Mouse Model of Fragile X Syndrome.

Fragile X Syndrome (FXS), a common inheritable form of intellectual disability, is known to alter neocortical circuits. However, its impact on the diverse synapse types comprising these circuits, or on the involvement of astrocytes, is not well known. We used immunofluorescent array tomography to quantify different synaptic populations and their association with astrocytes in layers 1 through 4 of the adult somatosensory cortex of a FXS mouse model, the FMR1 knockout mouse.

Triple differential cross sections for electron-impact ionization of methane at intermediate energy.

We report an experimental and theoretical investigation of electron-impact single ionization of the highest occupied molecular orbital 1t and the next highest occupied molecular orbital 2a states of CH at an incident electron energy of 250 eV. Triple differential cross sections measured in two different laboratories were compared with results calculated within the molecular 3-body distorted wave and generalized Sturmian function theoretical models. For ionization of the 1t state, the binary peak was observed to have a single maximum near the momentum transfer direction that evolved into a double peak for increasing projectile scattering angles, as has been seen for ionization of atomic p-states.

Low energy electron and positron impact differential cross sections for the ionization of water molecules in the coplanar and perpendicular kinematics.

We report here triply differential cross sections (TDCSs) for 81 eV electron and positron-impact ionization of the combined (1b + 3a) orbitals of the water molecule by using the second-order distorted wave Born approximation (DWBA2) for ejection electron and positron energies of 5 eV and 10 eV and different momentum transfer conditions. The electron-impact TDCS will be compared with the experimental data measured by Ren et al. [Phys.

Distinctive Structural and Molecular Features of Myelinated Inhibitory Axons in Human Neocortex.

Numerous types of inhibitory neurons sculpt the performance of human neocortical circuits, with each type exhibiting a constellation of subcellular phenotypic features in support of its specialized functions. Axonal myelination has been absent among the characteristics used to distinguish inhibitory neuron types; in fact, very little is known about myelinated inhibitory axons in human neocortex. Here, using array tomography to analyze samples of neurosurgically excised human neocortex, we show that inhibitory myelinated axons originate predominantly from parvalbumin-containing interneurons.

Electron impact ionization dynamics of para-benzoquinone.

Triple differential cross sections (TDCSs) for the electron impact ionization of the unresolved combination of the 4 highest occupied molecular orbitals (4b, 5b, 1b, and 2b) of para-benzoquinone are reported. These were obtained in an asymmetric coplanar geometry with the scattered electron being observed at the angles -7.5°, -10.

A deleterious Nav1.1 mutation selectively impairs telencephalic inhibitory neurons derived from Dravet Syndrome patients.

Dravet Syndrome is an intractable form of childhood epilepsy associated with deleterious mutations in SCN1A, the gene encoding neuronal sodium channel Nav1.1. Earlier studies using human induced pluripotent stem cells (iPSCs) have produced mixed results regarding the importance of Nav1.

Array tomography of physiologically-characterized CNS synapses.

Background: The ability to correlate plastic changes in synaptic physiology with changes in synaptic anatomy has been very limited in the central nervous system because of shortcomings in existing methods for recording the activity of specific CNS synapses and then identifying and studying the same individual synapses on an anatomical level.

New Method: We introduce here a novel approach that combines two existing methods: paired neuron electrophysiological recording and array tomography, allowing for the detailed molecular and anatomical study of synapses with known physiological properties.

Results: The complete mapping of a neuronal pair allows determining the exact number of synapses in the pair and their location.

Electron- and photon-impact ionization of furfural.

The He(i) photoelectron spectrum of furfural has been investigated, with its vibrational structure assigned for the first time. The ground and excited ionized states are assigned through ab initio calculations performed at the outer-valence Green's function level. Triple differential cross sections (TDCSs) for electron-impact ionization of the unresolved combination of the 4a″  +  21a' highest and next-highest occupied molecular orbitals have also been obtained.

Oxygen regulation of breathing through an olfactory receptor activated by lactate.

Animals have evolved homeostatic responses to changes in oxygen availability that act on different timescales. Although the hypoxia-inducible factor (HIF) transcriptional pathway that controls long-term responses to low oxygen (hypoxia) has been established, the pathway that mediates acute responses to hypoxia in mammals is not well understood. Here we show that the olfactory receptor gene Olfr78 is highly and selectively expressed in oxygen-sensitive glomus cells of the carotid body, a chemosensory organ at the carotid artery bifurcation that monitors blood oxygen and stimulates breathing within seconds when oxygen declines.

Long-term care planning and preferences among Japanese American baby boomers: Comparison with non-Japanese Americans.

Aims: Nikkei (Japanese American) communities are known to be forerunners for building culturally sensitive long-term care (LTC) services and programs. Existing literature highlights evolving cultural shifts among Nikkei communities as well as the baby boomers from the previous aging cohort. The present study's primary purpose was to examine Japanese American (JA) boomers' perceptions about their LTC planning compared with their non-JA counterparts.

Reduction of Cardiac Arrests: The Experience of a Novel Service Centric Medical Emergency Team.

Objective: To determine whether a novel, service-centric, medical emergency team (MET) model can impact cardiac arrest (CA) rates.

Methods: A retrospective pre- vs. postintervention analysis was performed on patients ≥ 18 years who had a CA between 2007 and 2012.