Efficiency of dual siRNA-mediated gene therapy for intervertebral disc degeneration (IVDD).

Background Context: One of the common causes of low back pain is intervertebral disc degeneration. The pathophysiology of disc degeneration involves apoptosis of nucleus pulposes cells and degradation of extra cellular matrix (ECM). Caspase 3 plays a central role in apoptosis and the ADAMTS5 (A Disintegrin and Metalloproteinase with Thrombospondin motifs 5) gene plays a critical role in ECM degradation.

Chlorin e6 loaded lactoferrin nanoparticles for enhanced photodynamic therapy.

Photosensitizer (PS) mediated Photodynamic Therapy (PDT) is a preferred treatment modality for certain cancers. Some of the factors limiting the expansion of PDT to other clinical conditions are the aggregation of hydrophobic PS in aqueous media and the inefficient biodistribution of photosensitizers. Formulations containing the PS have overcome some of the limitations by controlling aggregation-dependent quenching of PS and by improving the biodistribution of PS.

Draft Genome Sequence of Entomopathogenic Strain Lak 1210, an Alkaliphilic Chitin Degrader.

We announce here the draft genome sequence of strain Lak 1210, isolated from mangrove soil. This alkaliphilic strain is an efficient chitin degrader and has the ability to control insects and inhibit phytopathogenic fungi. The assembly consists of 5,082,926 bp, with 4,321 protein-coding sequences and a GC content of 41.

Combinatorial Labeling Method for Improving Peptide Fragmentation in Mass Spectrometry.

Annotation of peptide sequence from tandem mass spectra constitutes the central step of mass spectrometry-based proteomics. Peptide mass spectra are obtained upon gas-phase fragmentation. Identification of the protein from a set of experimental peptide spectral matches is usually referred as protein inference.

Anchor-dependent lipofection with non-glycerol based cytofectins containing single 2-hydroxyethyl head groups.

Detailed structure-activity investigations aimed at probing the anchor chain length dependency for glycerol-based lipofectins have been reported previously. Herein, we report on the first detailed investigation on the anchor-dependent transfection biology of non-glycerol based simple monocationic cytofectins containing single 2-hydroxyethyl head group functionality using 11 new structural analogs of our previously published first generation of non-glycerol based transfection lipids (lipids 1-11). The C-14 and C-16 analogs of DOMHAC (lipids 4 and 5, respectively) were found to be remarkably efficient in transfecting COS-1 cells.