Oxidative stress and regulation of Pink1 in zebrafish (Danio rerio).

Oxidative stress-mediated neuronal dysfunction is characteristic of several neurodegenerative disorders, including Parkinson's disease (PD). The enzyme tyrosine hydroxylase (TH) catalyzes the formation of L-DOPA, the rate-limiting step in the biosynthesis of dopamine. A lack of dopamine in the striatum is the most characteristic feature of PD, and the cause of the most dominant symptoms.

Dopaminergic cell damage and vulnerability to MPTP in Pink1 knockdown zebrafish.

The functions of PTEN (phosphatase/tensin homolog)-induced putative kinase (PINK1), which is mutated in early-onset Parkinson's disease, are poorly understood. We characterized a PINK1 antibody and found colocalization of PINK1-like immunoreactivity with aminergic markers. We inactivated translation of Pink1 using morpholino-oligonucleotides (MOs) in larval zebrafish.

Complementary developmental expression of the two tyrosine hydroxylase transcripts in zebrafish.

Tyrosine hydroxylase (TH) is a rate-limiting enzyme in the biosynthesis of catecholamines. In zebrafish, two genes encoding TH have been identified. We cloned them and studied their expression in zebrafish.