Publications by authors named "Madhuri S Lahamge"
Article Synopsis
- Buffaloes play a vital role in agriculture, but their mitochondrial biology is under-researched, leading to a study that looks at how the SDH gene is expressed in different buffalo tissues (kidneys, hearts, brains, and ovaries).
- Using RNA-seq data, researchers analyzed the expression of SDH subunits and assembly factors, identifying significant variations across tissues and their implications for energy metabolism and buffalo health.
- The results showed distinct expression patterns with specific up-regulations linked to mitochondrial functions, particularly highlighting important variations in the ovary that could inform better livestock management practices.
Background: The Murrah buffalo, pivotal in Asian agriculture, faces challenges in maximizing milk production despite significant breeding efforts. Recognizing its economic importance, this study investigates mtDNA D-loop variations in Murrah buffalo as potential indicators of milk production variability, addressing challenges in maximizing yield despite significant breeding efforts.
Methods And Results: Analyzing mtDNA D-loop sequences from 50 buffaloes, we categorized them into Low (Group 1), Medium (Group 2), and High ECM (Group 3) groups based on milk yields, fat and protein percentage of a 30-day period data.
Buffalo physiology intricately balances energy, profoundly influencing health, productivity, and reproduction. This study explores nuclear-mitochondrial crosstalk, revealing OXPHOS Complex I gene expression variations in buffalo tissues through high-throughput RNA sequencing. Unveiling tissue-specific disparities, the research elucidates the genomic landscape of crucial energy production genes, with broader implications for veterinary and agricultural progress.
View Article and Find Full Text PDFThe physiological well-being of buffaloes, encompassing phenotypic traits, reproductive health, and productivity, depends on their energy status. Mitochondria, the architects of energy production, orchestrate a nuanced interplay between nuclear and mitochondrial domains. Oxidative phosphorylation complexes and associated proteins wield significant influence over metabolic functions, energy synthesis, and organelle dynamics, often linked to tissue-specific pathologies.
View Article and Find Full Text PDFBackground: Cellular metabolism is most invariant process, occurring in all living organisms, which involves mitochondrial proteins from both nuclear and mitochondrial genomes. The mitochondrial DNA (mtDNA) copy number, protein-coding genes (mtPCGs) expression, and activity vary between various tissues to fulfill specific energy demands across the tissues.
Methods And Results: In present study, we investigated the OXPHOS complexes and citrate synthase activity in isolated mitochondria from various tissues of freshly slaughtered buffaloes (n = 3).