Drug product Formulation and Fill/Finish Manufacturing Process Considerations for AAV-Based Genomic Medicines.

Adeno-associated viruses (AAVs) have become the delivery medium of choice for a variety of genomic medicine applications i.e., gene therapy, gene editing/regulation, and ex-vivo cell therapy.

Scavenging amyloid oligomers from neurons with silica nanobowls: Implications for amyloid diseases.

Amyloid-β (Aβ) oligomers are toxic species implicated in Alzheimer's disease (AD). The prevailing hypothesis implicates a major role of membrane-associated amyloid oligomers in AD pathology. Our silica nanobowls (NB) coated with lipid-polymer have submicromolar affinity for Aβ binding.

Lipid-Encapsulated Silica Nanobowls as an Efficient and Versatile DNA Delivery System.

Nonmesoporous Janus silica nanobowls (NBs) are unique in that they possess two different nonporous surfaces per particle for loading biological molecules and can thus be designed with multifunctional properties. Although silica NBs have been successfully employed for both targeted therapeutic and diagnostic applications, their ability to deliver DNA has not yet been fully explored. The purpose of this study was to design and develop an in vitro transfection agent that would exploit the distinct characteristics of the silica NB.

Colloidal Plasmonic Nanocomposites: From Fabrication to Optical Function.

Plasmonic nanostructures are extensively used building blocks for engineering optical materials and device architectures. Plasmonic nanocomposites (pNCs) are an emerging class of materials that integrate these nanostructures into hierarchical and often multifunctional systems. These pNCs can be highly customizable by modifying both the plasmonic and matrix components, as well as by controlling the nano- to macroscale morphology of the composite as a whole.

Rapid ratiometric biomarker detection with topically applied SERS nanoparticles.

Multiplexed surface-enhanced Raman scattering (SERS) nanoparticles (NPs) offer the potential for rapid molecular phenotyping of tissues, thereby enabling accurate disease detection as well as patient stratification to guide personalized therapies or to monitor treatment outcomes. The clinical success of molecular diagnostics based on SERS NPs would be facilitated by the ability to accurately identify tissue biomarkers under time-constrained staining and detection conditions with a portable device. , and experiments were performed to optimize the technology and protocols for the rapid detection (0.

Method for assessing the reliability of molecular diagnostics based on multiplexed SERS-coded nanoparticles.

Surface-enhanced Raman scattering (SERS) nanoparticles have been engineered to generate unique fingerprint spectra and are potentially useful as bright contrast agents for molecular diagnostics. One promising strategy for biomedical diagnostics and imaging is to functionalize various particle types ("flavors"), each emitting a unique spectral signature, to target a large multiplexed panel of molecular biomarkers. While SERS particles emit narrow spectral features that allow them to be easily separable under ideal conditions, the presence of competing noise sources and background signals such as detector noise, laser background, and autofluorescence confounds the reliability of demultiplexing algorithms.