Oxidation of biological molecules with age and induced oxidative stress in different growth phases of Saccharomyces cerevisiae.

One of the mechanistic approaches for explaining ageing is the oxidative stress theory of ageing. Saccharomyces cerevisiae has been used as a model to study ageing due to many factors. We have attempted to investigate if the differential ability to withstand oxidative stress can be correlated with their lifespans.

Protein extraction from Saccharomyces cerevisiae at different growth phases.

Saccharomyces cerevisiae is an established model organism with a well characterized genome. However, this model presents a unique problem due to a very resistant cell wall which develops in the late stationary phase resulting in sub-optimal extraction of proteins from such cells using majority of the cell lysis protocols. In this study, several methods from the literature with modifications thereof for lysis of S.

Fluorescent Small Molecules Are BIG Enough To Sense Biomacromolecule: Synthesis of Aromatic Thioesters and Understanding Their Interactions with ctDNA.

The visible fluorescent chromophoric moiety present in the water-soluble photoactive yellow protein (PYP) of is -hydroxycinnamic acid linked to the cysteine residue (Cys-69) by a thioester bond and it controls the key photoinduced biological processes of the host organism. In the present work, we have synthesized and characterized three structurally different thiophenyl esters [viz., hydroxycinnamic-thiophenyl ester (), -,-dimethylaminocinnamic-thiophenyl ester (), and -phenyl-3-(4-chlorophenyl)-3-(phenylthio)propanethioate ()] in addition to a novel (to the best of our knowledge) stilbene-type olefinic compound, ,,,-tetramethyl-1,2-bis(phenylthio)ethene-1,2-diamine (), under the same reaction condition.