Optimizing autoclave pretreatment and inoculum-to-substrate ratio for enhanced anaerobic digestion of poultry slaughterhouse wastewater.

The poultry slaughter industry's substantial water usage and wastewater generation necessitate effective wastewater management strategies. This study explores the optimization of autoclave pretreatment and inoculum to substrate ratio (ISR) for improving the anaerobic digestion (AD) of poultry slaughterhouse wastewater (PSWW). Using response surface methodology (RSM), optimal conditions were determined to be a pH of 11, a pretreatment time of 60 min, and an ISR of 2.