In this study, we identified the in situ bacterial groups and their community structure in coastal waters influenced by anthropogenic inputs. The use of environmental DNA (eDNA) and high throughput sequencing (HTS) were employed to derive accurate and reliable information on bacterial abundance. The V3 and V4 hypervariable regions of the 16S rRNA gene were amplified and the sequences were clustered into operational taxonomic units to analyze the site-specific variations in community composition.
View Article and Find Full Text PDFTo understand the impact of seasonal variability on plankton food web composition in tropical coastal waters, samples were collected from three locations along Tuticorin coastal waters during postmonsoon, summer, and northeast (NE) monsoon seasons. During the NE monsoon, the total suspended matter (TSM) and nutrient concentrations were relatively higher, whereas salinity and plankton (phytoplankton and zooplankton) abundances were lower. Cluster analysis also revealed that the NE monsoon formed into a separate cluster because of the lower phytoplankton abundance caused by higher loads of TSM; this arrests light penetration, thereby resulting in a decrease in plankton abundance.
View Article and Find Full Text PDFAndaman and Nicobar islands is one of the major tourism hubs of the World. Most travelers visit these islands for historical attractions, beaches, snorkeling, scuba diving, coral reefs, adventure and recreation. Port Blair is the capital and sole entry/exit point of these islands.
View Article and Find Full Text PDFThe present investigation aimed to study the effect of monsoonal and anthropogenic influences on the water quality parameters of Puducherry coastal waters. Surface water sampling was performed at three fixed stations in four distinct seasons during 2011. Physical water quality parameters such as salinity and TSM showed strong seasonal and spatial variability.
View Article and Find Full Text PDFSulfated polysaccharide was isolated from Gracilaria debilis and purified through gel chromatography and their molecular weight was determined through AGE and PAGE. The total sugars in the crude, fractionated and purified polysaccharide were estimated as 52.65%, 59.
View Article and Find Full Text PDFβ-Chitosan extracted from the gladius of Sepioteuthis lessoniana was sulfated with chlorosufonic acid. The structural features of sulfated chitosan were analyzed by NMR spectroscopy. The elemental composition was analyzed using elemental analyzer.
View Article and Find Full Text PDFβ-Chitin and its chitosan from the gladius of Sepioteuthis lessoniana have been isolated, purified, characterised and compared with the commercial chitosan. Ash, moisture, mineral, metal and elemental content were analyzed using standard techniques. The optical activity of chitin was found to be levorotatory.
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