Production and characterization of virus-free, CRISPR-CAR T cells capable of inducing solid tumor regression.

Background: Chimeric antigen receptor (CAR) T cells have demonstrated high clinical response rates against hematological malignancies (e.g., CD19+ cancers) but have shown limited activity in patients with solid tumors.

Comparison of seven methods for DNA extraction from prosomata of the acorn barnacle, Amphibalanus amphitrite.

Next generation sequencing (NGS) technologies can provide an understanding of the molecular processes involved in marine fouling by Amphibalanus spp. barnacles. Here, seven methods for extracting DNA from A.

Correction: Effect of hydrogel material composition on hBMSC differentiation into zone-specific neo-cartilage: engineering human articular cartilage-like tissue with spatially varying properties.

Correction for 'Effect of hydrogel material composition on hBMSC differentiation into zone-specific neo-cartilage: engineering human articular cartilage-like tissue with spatially varying properties' by Kirsten Parratt et al., J. Mater.

Effect of hydrogel material composition on hBMSC differentiation into zone-specific neo-cartilage: engineering human articular cartilage-like tissue with spatially varying properties.

Biological tissues are complex structures with spatially distinct cellular compositions, architecture, and biochemical and mechanical properties. Therefore, it is imperative that biomaterial scaffolds which serve as frameworks for engineering tissue structures contain spatially-varying cues to differentiate encapsulated progenitor cells into distinct, spatially-organized phenotypes. Human articular cartilage consists of three spatially distinct zones: superficial, transitional, and middle, which have unique extracellular matrix (ECM) compositions, chondrocyte phenotypes, and mechanical properties.