What was old is new again: Phenotypic screening of a unique fungal library yields pyridoxatin, a promising lead against extensively resistant (AB5075).

Due to the emergence of resistance, the World Health Organization considers Gram-negative pathogen a top priority for therapeutic development. Using this priority pathogen and a phenotypic, agar plate-based assay, a unique library of extracts from 2,500 diverse fungi was screened for antimicrobial activity against a highly virulent, drug-resistant strain of (AB5075). The most potent hit from this screen was an extract from the fungus sp.