Publications by authors named "Madeline Febinger"
Comput Biol Chem
June 2024
Article Synopsis
- Protein variant libraries generated through site-directed mutagenesis help engineers create proteins with better properties, like enhanced activity and stability.
- These libraries are built by choosing specific residue positions and beneficial mutations, but the use of degenerate codons can introduce unwanted amino acids and premature STOP codons.
- Our study developed an algorithm to minimize the number of degenerate codons needed for desired amino acids and a dynamic method to efficiently create oligonucleotides, leading to a substantial increase in useful variants in mutant libraries at a slight increase in DNA synthesis costs.
