Use of poxvirus display to select antibodies specific for complex membrane antigens.

Antibody discovery against complex antigens is limited by the availability of a reproducible pure source of concentrated properly folded antigen. We have developed a technology to enable direct incorporation of membrane proteins such as GPCRs and into the membrane of poxvirus. The protein of interest is correctly folded and expressed in the cell-derived viral membrane and does not require any detergents or refolding before downstream use.

Unveiling the keratinolytic transcriptome of the black carpet beetle (Attagenus unicolor) for sustainable poultry feather recycling.

The black carpet beetle (BCB) is a household pest unique in its ability to digest complex proteins such as keratin that makes up the majority of feather structure. Despite voluminous yield and high protein content ( > 85%), feathers are poorly digested by most known organisms and are thereby rendered an environmental hazard. Furthermore, keratinolytic microbial strains are typically thermophilic and therefore economically and environmentally unsustainable.