Publications by authors named "Madeleine E Laws"
CRISPR/Cas9 methods are a powerful approach to edit the genome of . To convert existing lines to driver lines in a secondary school classroom setting, we applied the CRISPR-based genetic approach to a collection of 'driver' lines. The integration of the coat color marker into homology-assisted CRISPR knock-in (HACK) enabled visual selection of -to- conversions using brightfield stereo-microscopy available in a broader set of standard classrooms.
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- This study improved the CRISPR/Cas9 genome editing technique to convert Drosophila GAL4 lines into LexA lines using a new universal donor strain, simplifying the process of identifying successful edits with visual markers for microscopy.
- The research demonstrated consistent expression of a yellow transgene across various genomic locations after the LexA knock-in, while also unexpectedly increasing the expression of a red fluorescent protein transgene compared to previous methods.
- Collaborating with secondary school students, the team developed a workflow to efficiently create a collection of LexA lines from commonly used GAL4 lines, enhancing the tools available for gene expression studies.