Deletion of MGF505-2R Gene Activates the cGAS-STING Pathway Leading to Attenuation and Protection against Virulent African Swine Fever Virus.

African swine fever virus (ASFV) is the etiological agent causing African swine fever (ASF), affecting domestic pigs and wild boar, which is currently the biggest animal epidemic in the world and a major threat to the swine sector. At present, some safety concerns about using LAVs against ASFV still exist despite a commercial vaccine licensed in Vietnam. Therefore, the efforts to identify virulence factors and their mechanisms, as well as to generate new vaccine prototypes, are of major interest.

Molecular contamination of an animal facility during and after African swine fever virus infection.

Introduction: The molecular contamination of an animal facility was investigated during and after an infection with highly pathogenic African swine fever virus (ASFV) among domestic pigs. The investigation evaluated the risk of indirect transmission of the disease and indicated points that may facilitate cleaning and disinfection processes.

Material And Methods: Six domestic pigs were infected oronasally with the highly pathogenic Georgia 2007 strain.

A framework for the design, implementation, and evaluation of output-based surveillance systems against zoonotic threats.

Output-based standards set a prescribed target to be achieved by a surveillance system, but they leave the selection of surveillance parameters, such as test type and population to be sampled, to the responsible party in the surveillance area. This allows proportionate legislative surveillance specifications to be imposed over a range of unique geographies. This flexibility makes output-based standards useful in the context of zoonotic threat surveillance, particularly where animal pathogens act as risk indicators for human health or where multiple surveillance streams cover human, animal, and food safety sectors.

Comparison Study of Four Extraction Methods Combined with PCR and LAMP for Feline Detection in Fecal Samples.

Feline trichomonosis occurs worldwide, with gastrointestinal symptoms such as chronic large-bowel diarrhea and abdominal pain. The inclusion of molecular methods in diagnostic and epidemiological studies has necessitated an effective method for extracting DNA from feces. We tested four extraction commercial kits: ZR Fecal DNA MiniPrep (50 preps) (Zymo Research, Irvine, CA, USA), QIAamp DNA Stool Mini Kit (Qiagen Inc.

Proteomic Profiling and In Silico Characterization of the Secretome of Sensu Stricto L3 Larvae.

sensu stricto (s.s.) L3 larvae are one of the major etiological factors of human anisakiasis, which is one of the most important foodborne parasitic diseases.

Grass Snakes () as a Reservoir of and Other Parasites.

The aim of the study was to investigate the occurrence of (Goeze, 1782) in fifty-one grass snakes () collected in Gostynińsko-Włocławski Landscape Park. Each snake was tested for the presence of mesocercariae using the AMT and MSM methods. 18S ribosomal RNA (18S rRNA), cytochrome C oxidase subunit I (COI) and 28S ribosomal RNA (28S rRNA) genes were amplified by PCR and sequenced for the purpose of species identification.

Validation of the Magnetic Stirrer Method for the Detection of Larvae in Muscle Samples Based on Proficiency Tests Results.

is a zoonotic disease caused by the nematodes of the genus . Infection takes place through the consumption of infected meat containing live larvae. The only way to prevent the disease is to break its epizootic chain.

The First Record of (G5) Tapeworms in Grey Wolf ().

The aim of this study is to confirm the presence and molecular identification of tapeworms in wolves from south-eastern Poland. An investigation was carried out on the intestines of 13 wolves from south-eastern Poland. The small intestines were divided into three equal segments.

Unexpected Cross-Reaction with -Like DNA in a PCR for Detection of Bovine .

The prevalence of bovine infection has decreased almost to zero in most European countries, such as Poland, which has been -free since 1997. However, trichomonosis is a notifiable disease and there is a duty to examine samples from cattle. In this study, we present an unexpected cross-reaction with -like DNA in a specimen from a bull.

Development and Application of Novel Chemiluminescence Immunoassays for Highly Sensitive Detection of Proteins in Thermally Processed Seafood.

The third-stage larvae (L3) of are the most important source of hidden allergens in seafood products. However, there exist no commercial methods for detecting proteins in food. Furthermore, only a few methods have been validated for the detection of in thermally processed food.

Proteomic Profiling Reveals New Insights into the Allergomes of Anisakis simplex, Pseudoterranova decipiens, and Contracaecum osculatum.

Anisakis simplex, Pseudoterranova decipiens, and Contracaecum osculatum third-stage larvae (L3) are fish-borne nematodes that can cause human anisakidosis. Although A. simplex is a known source of allergens, knowledge about the allergic potential of P.

Proteomic and Bioinformatic Investigations of Heat-Treated Third-Stage Larvae.

third-stage larvae are the main source of hidden allergens in marine fish products. Some allergens are thermostable and, even highly processed, could cause hypersensitivity reactions. However, proteome has not been studied under autoclaving conditions of 121 °C for 60 min, which is an important process in the food industry.

Toxoplasma gondii infection in slaughtered pigs and cattle in Poland: seroprevalence, molecular detection and characterization of parasites in meat.

Background: Toxoplasma gondii infection may pose a severe medical problem especially in a congenital form and as an acquired infection in immunocompromised persons. Raw and undercooked meat of slaughtered animals is regarded as an important source of parasite infection; however, data concerning this issue in Poland are still insufficient. The aim of this study was to estimate the prevalence of T.

: A Study of Prevalence in Animal Hosts in Poland.

is described as a pathogen of cattle and cats and also exhibits commensalism with pigs. In order to estimate the prevalence and determine the risk factors for parasite infection, specimens from animal hosts (cat, pigs, and cattle) from Poland were investigated. To our best knowledge, this is the first such study to examine samples from wild boars () for the presence of Data were collected from 117 cats, 172 pigs, 236 wild boars, and 180 cattle.

Whole genome sequencing of a feline strain of Tritrichomonas foetus reveals massive genetic differences to bovine and porcine isolates.

Tritrichomonas foetus is a protozoan parasite that colonizes the reproductive tract of cattle as well as the gastrointestinal tract of cats. Bovine tritrichomonosis is a sexually transmitted disease whereas feline tritrichomonosis is thought to be transmitted by the fecal-oral route. Furthermore, T.

as a Causative Agent of Tritrichomonosis in Different Animal Hosts.

is a protozoan parasite that has been traditionally identified as a cause of reproductive tract disease in cattle and gastrointestinal tract infection in cats. Moreover, is also well known as a commensal of the nasal cavity, intestines, and stomach in swine. In this review we describe as a pathogen dangerous to more than one animal host, diagnostic and taxonomic aspects of this infection, and the extent to which isolates from different hosts share genetic identity.

First case of infection in beavers () in Poland and Europe.

Background: This is the first report of the finding of in beaver meat () in Poland and Europe. In Poland, the beaver is a strictly protected animal species, except the few regions where high population density leads to economic losses. In these areas, the reduction culling of the animals was introduced.

Development and comparative evaluation of different LAMP and PCR assays for coprological diagnosis of feline tritrichomonosis.

The protozoan parasite Tritrichomonas foetus may cause severe diarrhea in cats all over the world. In order to evaluate the methodology in coprological molecular diagnosis of feline tritrichomonosis, we compared previously published ("old") and newly developed ("novel") loop-mediated isothermal amplification (LAMP) (targeted to the T. foetus β-tubulin and the elf1α 1 gene, respectively) as well as an old conventional and an old and novel real-time PCR (all targeted to overlapping regions of T.

First report of Echinococcus multilocularis in cats in Poland: a monitoring study in cats and dogs from a rural area and animal shelter in a highly endemic region.

Background: Alveolar echinococcosis is a dangerous zoonotic disease caused by larval forms of Echinococcus multilocularis. In its life-cycle, the principal definitive host is the red fox; however, domesticated carnivorous animals (dogs and cats) can also act as definitive hosts. Until now, there were no data concerning this infection in cats in Poland.

Comparative analysis of excretory-secretory antigens of Anisakis simplex, Pseudoterranova decipiens and Contracaecum osculatum regarding their applicability for specific serodiagnosis of human anisakidosis based on IgG-ELISA.

Serodiagnosis of human anisakidosis is presently hampered by the current lack of standardised serological assays that allow sensitive and specific detection of Anisakidae-specific antibodies in human patients. In the present study, we comparatively evaluated the diagnostic value (by IgG-ELISA) of excretory-secretory antigens (ESAgs) of Anisakis simplex, Pseudoterranova decipiens and Contracaecum osculatum, representing the most frequently found genera responsible for human infection. In addition, we tested also a mix of the three ES preparations (Mix-ESAgs) as well as two recombinant allergens of A.

Occurrence of Intestinal Parasites in Pigs in Poland - the Influence of Factors Related to the Production System.

Introduction: The aim of study was to estimate the prevalence and intensity of intestinal parasite infections in pigs in Poland and evaluate the influence of factors related to the production system on the infection intensity.

Material And Methods: A total of 70 pig farms of all Polish provinces, differing in the herd size and production system, were selected for the study. Fresh faecal samples were collected from all age groups: suckling piglets, weaners, fatteners, and lactating sows.