Chemostat enrichments of human feces with resistant starch are selective for adherent butyrate-producing clostridia at high dilution rates.

Resistant starch (RS) enrichments were made using chemostats inoculated with human feces from two individuals at two dilution rates (D = 0.03 h(-1) and D = 0.30 h(-1)) to select for slow- and fast-growing amylolytic communities.

Role of mechanical and psychosocial factors in the onset of forearm pain: prospective population based study.

Objective: To determine the aetiology of forearm pain. In particular to determine the relative contribution of (a) psychological factors, features of somatisation, and health anxiety and behaviour, (b) work related mechanical factors, and (c) work related psychosocial factors in the onset of forearm pain.

Design: 2 year prospective population based cohort study, with retrospective assessment of exposures at work.

The Semaphore crab, Heloecius cordiformis: bio-indication potential for heavy metals in estuarine systems.

Although alterations at the organism level in decapod crustaceans on exposure to heavy metals have been evidenced in the laboratory, little examination of metal effects on morphology and population parameters have been explored in a field-based situation. Relationships between morphological parameters, population demography and heavy metal sediment loadings were examined in conjunction with the accumulation of metals in the Semaphore crab, Heloecius cordiformis, in the Port Jackson and Hawkesbury River estuaries, Sydney, Australia. H.

Radiographic change is common in new presenters in primary care with hip pain. PCR Hip Study Group.

Objective: To determine the prevalence of radiographic osteoarthritis in subjects with hip pain newly presenting to primary care.

Methods: The study was cross-sectional in design, set in 35 general practices across the UK. It included 195 men and women aged 40 yr and over (median 63 yr) presenting with a new episode of hip pain.

Increasing incidence of oral cancer amongst young persons: what is the aetiology?

The reasons for an increasing incidence of oral cancer, particularly amongst younger persons is unclear. It has been hypothesised either to be a result of an increase in exposure to known risk factors amongst certain groups in the community, or to be due to new aetiological agents. Prior to conducting large expensive population-based studies, it seems appropriate to conduct initial smaller-scale surveys to assess evidence for each of these two hypotheses.

Mortality among UK Gulf War veterans.

Background: Armed forces personnel who served in the Gulf War report more current ill-health than those who were not deployed. There has been concern expressed that they may also experience higher mortality rates.

Methods: A retrospective cohort study was done including all 53462 UK Gulf War veterans (Gulf cohort) and a comparison group equivalent in size of personnel who were not deployed but matched for age, sex, rank, service, and level of fitness (Era cohort).

Effects of covariate measurement error in the initial level and rate of change of an exposure variable.

When repeated measures of an exposure variable are obtained on individuals, it can be of epidemiologic interest to relate the slope of this variable over time to a subsequent response. Subject-specific estimates of this slope are measured with error, as are corresponding estimates of the level of exposure, i.e.

Occupational risk factors for shoulder pain: a systematic review.

Objectives: To systematically evaluate the available evidence on occupational risk factors of shoulder pain.

Methods: Relevant reports were identified by a systematic search of Medline, Embase, Psychlit, Cinahl, and Current Contents. The quality of the methods of all selected publications was assessed by two independent reviewers using a standardised checklist.

The association between chronic widespread pain and mental disorder: a population-based study.

Objective: Patients with chronic widespread pain (CWP) have been reported to have a greater prevalence of mental disorders and somatization than that found in the general population, but the true association between CWP and mental disorders is unknown. In this study, we investigated whether there is an increased prevalence of mental disorder in people with CWP from the general population. We also describe the psychiatric diagnoses associated with CWP.

Development and validation of a questionnaire to assess disabling foot pain.

This study outlines the design and validation of a new self-administered instrument for assessing foot pain and disability. The 19-item questionnaire was tested on 45 rheumatology patients, 33 patients who had attended their general practitioner with a foot-related problem and 1000 responders to a population survey of foot disorders. Levels of reported disability were found to be greatest for rheumatology patients and least for community subjects.

Analysis of whole blood tacrolimus concentrations in liver transplant patients exhibiting impaired liver function.

In transplant patients with impaired liver function, HPLC methodologies have been suggested for monitoring whole blood tacrolimus concentrations because of the reported inaccuracy of immunoassay for whole blood tacrolimus concentrations. One hundred fifty whole blood samples from 50 subjects enrolled in a multicenter liver transplant trial were chosen for HPLC/MS/MS analysis without consideration of the clinical status of the patient at the time of sampling. These samples were chosen to represent the sampling intervals during the 12-week posttransplantation period.

Generalized pain, fibromyalgia and regional pain: an epidemiological view.

Generalized pain, which is one component of the fibromyalgia syndrome, is a common and disabling problem in the general population. Pain at individual sites, such as the lower back and shoulder, has traditionally been considered distinct from generalized pain and studied separately. This chapter considers first the basic definition and distribution of widespread pain in the population, and second examines the evidence as to whether widespread and regional pain are truly or usefully distinguished.

Analytical validation of the PRO-Trac II ELISA for the determination of tacrolimus (FK506) in whole blood.

Background: The analytical validation of multiple lots of the PRO-Trac II ELISA (DiaSorin) for the determination of tacrolimus in whole blood is described.

Methods: The analytical parameters assessed included analytical sensitivity, dilution linearity, functional sensitivity, values in samples containing no tacrolimus, intra- and interassay precision, supplementation and recovery, metabolite cross-reactivity, interference studies, and method comparisons HPLC-tandem mass spectrometry (HPLC/MS/MS) and the IMx Tacrolimus II multiparticle enzyme immunoassay. Where appropriate, assessments were performed according to NCCLS guidelines.

Short-term physical risk factors for new episodes of low back pain. Prospective evidence from the South Manchester Back Pain Study.

Study Design: A prospective population-based cohort study performed in South Manchester, United Kingdom.

Objectives: To determine whether nonoccupational physical activity and indicators of physical stress on the spine predict low back pain in the short term.

Summary Of Background Data: There is evidence that physical activity outside the workplace helps to protect against low back pain in the long term.

Functional food properties of non-digestible oligosaccharides: a consensus report from the ENDO project (DGXII AIRII-CT94-1095).

This paper results from the final phase of the ENDO project (DGXII AIRII-CT94-1095), a European Commission-funded project on non-digestible oligosaccharides (NDO). All participants in the programme met to perform a consensus exercise on the possible functional food properties of NDO. Topics studied during the project (including a workshop on probiotics and prebiotics) and related aspects, for which considerable evidence has been generated recently, were evaluated on the basis of existing published scientific evidence.

The association between tender points, psychological distress, and adverse childhood experiences: a community-based study.

Objective: To examine the hypothesis that characteristics of somatization and illness behavior, and their childhood antecedents, are associated with the presence of multiple tender points.

Methods: Two hundred eighty-nine subjects who had demonstrated psychological distress (General Health Questionnaire score > or =2) had a tender point examination and in-depth psychological evaluation. In addition, subjects were interviewed about a number of adverse childhood experiences.

Predicting who develops chronic low back pain in primary care: a prospective study.

Objectives: To quantify the relative contribution of premorbid and episode specific factors in determining the long term persistence of disabling symptoms of low back pain.

Design: Prospective cohort study.

Setting: Two general practices in the south Manchester area.

The prevalence and associated features of chronic widespread pain in the community using the 'Manchester' definition of chronic widespread pain.

Objective: We examine the descriptive epidemiology of chronic widespread pain using the 'Manchester' definition [CWP(M)] and assess psychosocial and other features which characterize subjects with such pain according to these more stringent criteria.

Methods: A population postal survey of 3004 subjects was conducted in the Greater Manchester area of the UK.

Results: The point prevalence of Manchester-defined chronic widespread pain was 4.

Predictors of early improvement in low back pain amongst consulters to general practice: the influence of pre-morbid and episode-related factors.

Low back pain symptoms are extremely common. affecting as many as 80% of the population at some time in their lives. However, the majority of the medical costs arise from the minority of patients whose symptoms become chronic.

Chronic widespread pain in the community: the influence of psychological symptoms and mental disorder on healthcare seeking behavior.

Objective: To determine whether psychological symptoms and mental disorder are an intrinsic part of the chronic widespread pain syndrome or whether they have been observed in clinic attenders primarily because of their influence on the decision to seek a medical consultation.

Methods: A population survey of 1953 subjects was conducted in the Greater Manchester area of the United Kingdom. The survey included a postal questionnaire, and in a subgroup of respondents with high levels of distress, the presence of mental disorder was assessed by a semistructured standardized interview.

Ecological and physiological studies on large intestinal bacteria in relation to production of hydrolytic and reductive enzymes involved in formation of genotoxic metabolites.

Several hydrolytic and reductive bacterial enzymes (beta-glucuronidase, GN; beta-glucosidase, GS; arylsulphatase, AS; azoreductase, AR; nitroreductase, NR) involved in production of mutagenic or genotoxic metabolites were measured in human colonic contents. Cell-associated AS and extracellular GS were approximately twice as high in the distal colon compared with the proximal bowel, while AR changed little throughout the gut. Measurements of these enzymes in faeces from seven healthy donors confirmed that the majority were cell-associated, and demonstrated high levels of inter-individual variability.