An androgen receptor in rat brain and pituitary.

Dihydrotestosterone (DHT) binding was measured in cytosols from brain regions and pituitary of adult female rats and, with the addition of ventral prostate, in adult male rats. Two types of binding were distinguished: one, saturable at concentration of DHT greater than or equal to 5 X 10(-9) M and an unsaturable component. In intact males saturable (limited capacity) binding was detected only in ventral prostate cytosol; 3 days after orchidectomy the saturable binding sites increase 3-fold in prostate and in pituitary, hypothalamus, amygdala and cortex to detectable levels in approximately the same abundance as in females.

Physiological variation in abundance of oestrogen specific high-affinity binding sites in hypothalamus, pituitary and uterus of the rat.

High-affinity binding of [2,4,6,7-3-H]oestradiol-17 beta has been studied in cytosols prepared from hypothalami, pituitaries and uteri of female rats killed at different stages of the oestrous cycle, and in cytosols prepared from the hypothalami and pituitaries of male rats. In all cases the equilibrium dissociation constant of reaction was of the order of 10- minus 10 mol/1. The number of available high affinity sites per tissue (n) varied with physiological state.