The HRH4 rs11662595 mutation is associated with histamine H receptor dysfunction and with increased epithelial-to-mesenchymal transition progress in non-small cell lung cancer.

We previously demonstrated that histamine H receptor (HRH4) played important roles to suppress epithelial-to-mesenchymal transition (EMT) progress in non-small cell lung cancer (NSCLC). Furthermore, recent investigations suggested that genetic variations in HRH4 gene affected HRH4 function and eventually contributed to certain HRH4-related diseases. However, the relations between polymorphisms in HRH4 gene and NSCLC as well as their underlying mechanisms remain largely uninvestigated.

Ultrasonic synthesis of two nanostructured cadmium(II) coordination supramolecular polymers: Solvent influence, luminescence and photocatalytic properties.

Two nanoparticles of cadmium(II) coordination polymers (CPs) formulated as [Cd(L)(DCTP)] (1) and [Cd(L)(DCTP)·2HO] (2) (L=1,2-bis(2-methylbenzimidazol-1-ylmethyl)benzene, HDCTP=2,5-dichloroterephthalic acid) were prepared by the sonochemical approach in different solvents and characterized by elemental analysis, IR spectra, scanning electron microscopy (SEM), and powder X-ray diffraction. Structural determination reveals that CP 1 displays a 2D four-connected sql net layer, Whilst CP 2 exhibits a 1D "V"-like chain structure. Luminescence properties, thermal behavior, and photocatalytic activities of the nanoparticles of CPs 1 and 2 on the degradation of methylene blue were investigated.

An Improved Barcoded Oligonucleotide Primers-based Next-generation Sequencing Approach for Direct Identification of Viral Pathogens in Clinical Specimens.

Objective: To provide a feasible and cost-effective next-generation sequencing (NGS) method for accurate identification of viral pathogens in clinical specimens, because enormous limitations impede the clinical use of common NGS, such as high cost, complicated procedures, tremendous data analysis, and high background noise in clinical samples.

Methods: Viruses from cell culture materials or clinical specimens were identified following an improved NGS procedure: reduction of background noise by sample preprocessing, viral enrichment by barcoded oligonucleotide (random hexamer or non-ribosomal hexanucleotide) primer-based amplification, fragmentation-free library construction and sequencing of one-tube mixtures, as well as rapid data analysis using an in-house pipeline.

Results: NGS data demonstrated that both barcoded primer sets were useful to simultaneously capture multiple viral pathogens in cell culture materials or clinical specimens and verified that hexanucleotide primers captured as many viral sequences as hexamers did.

[Relationships of wheat seed vigor with enzyme activities and gene expression related to seed germination under stress conditions.].

This study aimed to analyze the enzyme activities and gene expression differences related to seed germination in different wheat genotypes, and to clarify the relationship between seed vigor and the related enzyme activities as well as gene expression under stress germination conditions. We measured seed vigor, total soluble sugar content, soluble protein content, α-amylase activity, cysteine protease activity and gene expression of the related enzymes of four wheat cultivars under drought, artificial aging and cold soaking stress. Results showed that drought, artificial aging and cold soaking stress affected seed vigor to some extent.

Role of selectins and their ligands in human implantation stage.

Selectins are a family of calcium-dependent, type I transmembrane, carbohydrate-binding glycoproteins. Selectins and their ligands are not only involved in physiological processes such as leukocyte homing and pathological processes such as cancer, but also play an essential role in the human implantation. L-selectin and its ligands participate in the adhesion of the blastocyst to the endometrium at the maternal-fetal interface.

The Complete Maternally and Paternally Inherited Mitochondrial Genomes of a Freshwater Mussel Potamilus alatus (Bivalvia: Unionidae).

Doubly uniparental inheritance (DUI) of mitochondrial DNA, found only in some bivalve families and characterized by the existence of gender-associated mtDNA lineages that are inherited through males (M-type) or females (F-type), is one of the very few exceptions to the general rule of strict maternal mtDNA inheritance in animals. M-type sequences are often undetected and hence still underrepresented in the GenBank, which hinders the progress of the understanding of the DUI phenomenon. We have sequenced and analyzed the complete M and F mitogenomes of a freshwater mussel, Potamilus alatus.

Construction of a high-density genetic map using specific-locus amplified fragments in sorghum.

Background: Sorghum is mainly used as a human food and beverage source, playing an important role in the production of ethanol and other bio-industrial products. Thus it is regarded as a model crop for energy plants. Genetic map construction is the foundation for marker-assisted selection and gene cloning.

Scutellarin-graft cationic β-cyclodextrin-polyrotaxane: Synthesis, characterization and DNA condensation.

As a prerequisite of gene delivery in living cells, DNA condensation has attracted more and more attention. In order to improve the efficiencies of polyamine-β-cyclodextrin-based cationic polyrotaxanes (PR-EDA and PR-DETA) as DNA condensation materials, we have designed and prepared two novel scutellarin-grafted cationic polyrotaxanes (PR-EDA-SCU and PR-DETA-SCU), in which scutellarins (SCU), the planar molecules, were conjugated on the cyclodextrin molecules of PR-EDA and PR-DETA. These materials were characterized by 1D and 2D NMR, XRD, TG and DSC.

The novel composite mechanism of ammonium molybdophosphate loaded on silica matrix and its ion exchange breakthrough curves for cesium.

Long-lived Cs (over 30 years), a byproduct of the spent fuel fission processes, comprises the majority of high-level and prolific heat-generating waste in downstream processing. This study reports a novel sequential annealing mechanism with cross-linked network of polyvinyl alcohol, fabricating the composite of ammonium molybdophosphate loaded on silica matrix (SM-AMP20, 20wt% AMP) as an excellent granular ion exchanger for removal Cs. When the matrix is remarkably sequential annealed, well-dispersed SM-AMP20 particles are formed by firmly anchoring themselves on controlling the porous characteristics and stable structure.

High internal ionic liquid phase emulsion stabilized by metal-organic frameworks.

The emulsification of metal-organic frameworks (MOFs) for the two immiscible phases of water and ionic liquid (IL) was investigated for the first time. It was found that Ni-BDC (BDC = 1,4-dicarboxybenzene) can emulsify water and ILs and favor the formation of high internal phase emulsions (HIPEs) under certain experimental conditions. The microstructures of the HIPEs were characterized by confocal laser scanning microscopy using a fluorescent dye Rhodamine B, which proves that the HIPEs are the IL-in-water type.

[CLITOROPLASTY FOR GENITAL VIRILIZATION IN GIRLS WITH CONGENITAL ADRENAL HYPERPLASIA].

Objective: To investigate the effectiveness of clitoroplasty of reserving the clitoris dorsal neurovascular bundle and the ventral complete urethral plate for genital virilization in girls with congenital adrenal hyperplasia (CAH).

Methods: Between June 2000 and October 2015, 27 girls with genital virilization were treated with clitoroplasty of reserving the clitoris dorsal neurovascular bundle and the ventral complete urethral plate. The chromosomal karyotype was 46, XX in all patients, whose age ranged from 3 to 11 years (mean, 3.

[Seed vigor evaluation based on adversity resistance index of wheat seed germination under stress conditions.].

A total of 16 wheat cultivars were selected to detect seed vigor of different genotypes using standard germination test, seed germination test under stress conditions and field emergence test. The adversity resistance indices of seed vigor indices and field emergence percentage under different germination conditions were used as the indices to evaluate adversity resistance. Principal component analysis and cluster analysis were used for the comprehensive evaluation of seed vigor.

A Computerized Evaluation of Sensory Memory and Short-term Memory Impairment After Rapid Ascent to 4280 m.

To evaluate the effect of acute high-altitude exposure on sensory and short-term memory using interactive software, we transported 30 volunteers in a sport utility vehicle to a 4280 m plateau within 3 h. We measured their memory performance on the plain (initial arrival) and 3 h after arrival on the plateau using six measures. Memory performance was significantly poorer on the plateau by four of the six measures.

[Maize biomass simulation based on dynamic photosynthate allocation.].

Photosynthate allocation is critical to crop growth and biomass formation, and it is also a key part of crop simulation. A photosynthate allocation model of maize was established based on photosynthate allocation mechanisms as well as the photosynthate allocation characteristics of maize at different stages. This model was then coupled with the CO assimilation module of WOFOST crop model to simulate the biomass dynamics of different organs of maize on daily scale.

[Characteristics of phosphate-solubilizing microbial community in the soil of poplar plantations under successive-planting and rotation.].

The metagenome sequencing was used to compare the difference of six soil samples, the rhizosphere soil of the first and the second rotation poplar plantations (RSP1, RSP2), the bulk soil of the first and the second rotation poplar plantations (BSP1, BSP2), the soil of rotated peanut field (RPS) and the abandoned land soil (ALS) after poplar clear cutting, in phosphate-solubili-zing microbial community and abundance of phosphatase gene (PG) in a poplar plantation. The results showed that microorganisms from nine genera were related to phosphorus cycle with Bacillus and Pseudomonas being the dominant. The abundance of PSMs was highest in ALS, followed by RPS, RSP2 and BSP2, RSP1 and BSP1, respectively.

[Fluorescence Properties and the Detection of Benzaldehyde of Lanthanide Complex with 2-Sufoterephthalalic Acid].

A new lanthanide coordination polymer, [Eu(2-stp) (2,2'-bipy) (H₂O)] · H₂O (2-stp = 2-sufoterephthalalic acid, 2, 2'-bipy=2,2'-bipyridine) was obtained by hydrothermal method. The crystal structure was determined by single crystal X-ray diffraction. The complex has a one-dimensional parallel double-chain structure.

Cellular graphene aerogel combines ultralow weight and high mechanical strength: A highly efficient reactor for catalytic hydrogenation.

The construction of three-dimensional graphene aerogels (GAs) is of great importance owing to their outstanding properties for various applications. Up to now, the combination of ultralow weight and super mechanical strength for GA remains a great challenge. Here we demonstrate the fabrication of cellular GAs by a facile, easily controlled and versatile route, i.

Sialylation Facilitates the Maturation of Mammalian Sperm and Affects Its Survival in Female Uterus.

Establishment of adequate levels of sialylation is crucial for sperm survival and function after insemination; however, the mechanism for the addition of the sperm sialome has not been identified. Here, we report evidence for several different mechanisms that contribute to the establishment of the mature sperm sialome. Directly quantifying the source of the nucleotide sugar CMP-beta-N-acetylneuraminic acid in epididymal fluid indicates that transsialylation occurs in the upper epididymis.

A New Modification of the Koyanagi Technique for the One-stage Repair of Severe Hypospadias.

Objective: To describe a new modification of the Koyanagi technique for the one-stage repair of severe hypospadias and its short-term outcomes.

Patients And Methods: Our modified Koyanagi technique was performed in 24 patients with severe hypospadias between February 2012 and January 2015. The age of the patients ranged from 1.

Ginsenoside Reduces Cognitive Impairment During Chronic Cerebral Hypoperfusion Through Brain-Derived Neurotrophic Factor Regulated by Epigenetic Modulation.

Increased expression of brain-derived neurotrophic factor (BDNF) has been associated with memory-enhancing and neuroprotective properties of some drugs under chronic cerebral hypoperfusion (CCH) condition. Ginsenoside Rd (GSRd), one of the main active ingredients in Panax ginseng, is widely used for brain protection. However, it is poorly understood whether epigenetic mechanisms implied in the BDNF modulation after GSRd treatment for CCH remain elusive.

Activation of GLP-1 Receptor Promotes Bone Marrow Stromal Cell Osteogenic Differentiation through β-Catenin.

Glucagon-like peptide 1 (GLP-1) plays an important role in regulating bone remodeling, and GLP-1 receptor agonist shows a positive relationship with osteoblast activity. However, GLP-1 receptor is not found in osteoblast, and the mechanism of GLP-1 receptor agonist on regulating bone remodeling is unclear. Here, we show that the GLP-1 receptor agonist exendin-4 (Ex-4) promoted bone formation and increased bone mass and quality in a rat unloading-induced bone loss model.

High-internal-phase emulsions stabilized by metal-organic frameworks and derivation of ultralight metal-organic aerogels.

To design high-internal-phase emulsion (HIPE) systems is of great interest from the viewpoints of both fundamental researches and practical applications. Here we demonstrate for the first time the utilization of metal-organic framework (MOF) for HIPE formation. By stirring the mixture of water, oil and MOF at room temperature, the HIPE stabilized by the assembly of MOF nanocrystals at oil-water interface could be formed.

An Orally Active Allosteric GLP-1 Receptor Agonist Is Neuroprotective in Cellular and Rodent Models of Stroke.

Diabetes is a major risk factor for the development of stroke. Glucagon-like peptide-1 receptor (GLP-1R) agonists have been in clinical use for the treatment of diabetes and also been reported to be neuroprotective in ischemic stroke. The quinoxaline 6,7-dichloro-2-methylsulfonyl-3-N-tert- butylaminoquinoxaline (DMB) is an agonist and allosteric modulator of the GLP-1R with the potential to increase the affinity of GLP-1 for its receptor.