Identification and expression analysis of lncRNAs in rice roots (Oryza sativa L.) under elevated CO concentration and/or cadmium stress.

The gradual rise of CO is one of the global climate changes, Cd stress is also a major abiotic stress factor that affects rice (Oryza sativa L.). The rice seedlings were treated under two CO concentrations and two CdCl concentrations for 7 days (treatments names: 400 ± 20 μmol mol CO and 0 μmol L CdCl concentrations, AC; 400 ± 20 μmol mol CO and 150 μmol L CdCl concentrations, Cd; 800 ± 20 μmol mol CO and 0 μmol L CdCl concentrations, EC; 800 ± 20 μmol mol CO and 150 μmol L CdCl concentrations, EC + Cd).

Polyethyleneimine-mediated assembly of DNA nanotubes for KRAS siRNA delivery in lung adenocarcinoma therapy.

Self-assembled DNA nanostructures hold great promise in biosensing, drug delivery and nanomedicine. Nevertheless, challenges like instability and inefficiency in cellular uptake of DNA nanostructures under physiological conditions limit their practical use. To tackle these obstacles, this study proposes a novel approach that integrates the cationic polymer polyethyleneimine (PEI) with DNA self-assembly.

An ultrasensitive solid-state ECL biosensor based on synergistic effect between Zn-NGQDs and porphyrin-based MOF as "on-off-on" platform.

To develop an ultra-sensitive solid-state electrochemiluminescence (ECL) biosensor for detection of miRNA 24, three different forms of porphyrin metal-organic framework (MOF) nanomaterials with good biocompatibility were synthesized through small molecule ligand modulation. We investigated various properties of synthesized MOFs in the presence of different small molecule ligands. The as-obtained 2D MOF nanodisk exhibited high ECL intensity and outstanding stability in the presence of a co-reactant at low concentrations.

Transcriptome modulation by endophyte drives rice seedlings response to Pb stress.

This study investigated the growth, SPAD value, chlorophyll fluorescence and transcriptome response of endophyte uninoculated and inoculated rice seedlings under Pb stress after treatment of 1 d and 5 d. Inoculation of endophytes significantly improved the plant height, SPAD value, F/F F/F and PI by 1.29, 1.

Neuroprotective Effects of Sodium Butyrate by Restoring Gut Microbiota and Inhibiting TLR4 Signaling in Mice with MPTP-Induced Parkinson's Disease.

Parkinson's disease (PD) is a prevalent type of neurodegenerative disease. There is mounting evidence that the gut microbiota is involved in the pathogenesis of PD. Sodium butyrate (NaB) can regulate gut microbiota and improve brain functioning in neurological disorders.

Application of RNA sequencing to understand the response of rice seedlings to salt-alkali stress.

Background: Salt-alkali stress represents one of the most stressful events with deleterious consequences for plant growth and crop productivity. Despite studies focusing on the effects of salt-alkali stress on morphology and physiology, its molecular mechanisms remain unclear. Here, we employed RNA-sequencing (RNA-seq) to understand how NaCO stress inhibits rice seedling growth.

Metabolomics analysis reveal the molecular responses of high CO concentration improve resistance to Pb stress of Oryza sativa L. seedlings.

Rice seedlings were exposed to two CO concentrations (400 ± 20 and 800 ± 20 μmol mol) and three PbNO concentrations (0, 50 and 100 µmol L) for 10 days to explore the regulatory mechanisms of elevated CO for Pb stress resistance. Electrical conductivity, MDA content, SOD, POD, CAT activities and metabolomics changes were studied. Results showed that: Pb stress damaged cell membrane system, electrical conductivity and MDA content increased 49.

A novel sensor for visual and selective detection of Hg based on functionalized doped quantum dots.

In this paper, a novel analytical platform for the visual, sensitive and reliable analysis of mercury ions (Hg) is fabricated based on functionalized doped quantum dots. We synthesized a new specific nano-material, zinc dithiothreitol combined with graphene quantum dots (ZnNCs-NGQDs), by a simple and convenient method which, as an efficient luminophore, was then applied to construct an electrochemiluminescence (ECL) system for the first time. Under optimized conditions, the ECL sensor showed an excellent response for Hg in the linear range of 1.

Toward efficient multiple-site incorporation of unnatural amino acids using cell-free translation system.

Amber suppression has been widely used to incorporate unnatural amino acids (UNAAs) with unique structures or functional side-chain groups into specific sites of the target protein, which expands the scope of protein-coding chemistry. However, this traditional strategy does not allow multiple-site incorporation of different UNAAs into a single protein, which limits the development of unnatural proteins. To address this challenge, the suppression method using multiple termination codons (TAG, TAA or TGA) was proposed, and cell-free unnatural protein synthesis (CFUPS) system was employed.

Whole genome sequencing analysis of a dexamethasone-degrading Burkholderia strain CQ001.

This study is to analyze the functional genes and metabolic pathways of dexamethasone degradation in Burkholderia through genome sequencing.A new Burkholderia sp. CQQ001 (B.

Endophyte infection enhances accumulation of organic acids and minerals in rice under Pb stress conditions.

This study aimed to assess the effects of endophyte infection on Pb accumulation, organic acids (OAs) and mineral element contents by comparing endophyte-uninfected (E-) and endophyte-infected (E+) rice exposed to 0, 10.36, 20.72, 31.

β-Caryophyllene/Hydroxypropyl-β-Cyclodextrin Inclusion Complex Improves Cognitive Deficits in Rats with Vascular Dementia through the Cannabinoid Receptor Type 2 -Mediated Pathway.

This work was conducted to prepare β-caryophyllene-hydroxypropyl-β-cyclodextrin inclusion complex (HPβCD/BCP) and investigate its effects and mechanisms on cognitive deficits in vascular dementia (VD) rats. First, HPβCD/BCP was prepared, optimized, characterized, and evaluated. HPβCD/BCP and AM630 were then administered to VD rats to upregulate and downregulate the cannabinoid receptor type 2 (CB2).

Degradation of dexamethasone by acclimated strain of Pseudomonas Alcaligenes.

This study is to investigate the use of microbial remediation technology for degradation of dexamethasone in polluted water. A strain of Pseudomonas Alcaligenes with the ability of dexamethasone degradation was isolated from hospital polluted water. This strain was further acclimated into a bacterial strain that could highly degrade dexamethasone.

Isolation and identification of dexamethasone sodium phosphate degrading Pseudomonas alcaligenes.

Glucocorticosteroids such as dexamethasone have polluted hospital wastewater, urban sewage, and river water in varying degrees. However, dexamethasone degradation by bioremediation technology is less understood. This study aims to isolate bacteria that could degrade dexamethasone and to identify their degradation characteristics.

[Studies on screening of matrix and permeation enhancer of sustained-release patches of duliang].

Objective: To screen the matrix and permeation enhancer of Duliang Patches.

Methods: Based on L9 (3(4)) orthogonal experimental design, the content of imperatorin of the release rate and transdermal osmolality were regarded as evaluation indexes to optimize the matrix and permeation enhancer of patches suing of Drug dissolution tester and Franz diffusion cell.

Results: The best prescriplion of sustained-release patch of Duliang was: the quality percentage content of the starch, sodium carboxymethyl cellulose, glycerin, azone, propylene glycol and PEG400 was 6%, 2%, 30%, 1%, 15% and 2.

Growth, photosynthesis and antioxidant responses of endophyte infected and non-infected rice under lead stress conditions.

An endophytic fungus was tested in rice (Oryza sativa L.) exposed to four levels of lead (Pb) stress (0, 50, 100 and 200 μM) to assess effects on plant growth, photosynthesis and antioxidant enzyme activity. Under Pb stress conditions, endophyte-infected seedlings had greater shoot length but lower root length compared to non-infected controls, and endophyte-infected seedlings had greater dry weight in the 50 and 100 μM Pb treatments.

Effects of Na₂CO₃ stress on photosynthesis and antioxidative enzymes in endophyte infected and non-infected rice.

Endophyte infected and uninfected seedlings of rice (Oryza sativa L.) were subjected to five different levels of Na(2)CO(3) for 2 weeks. Under both Na(2)CO(3) stress as well as no stress, endophyte-infected plants were higher for above-ground dry weight and shoot length, but lower for root length compared to non-infected controls, and there was no significant difference for below-ground dry weight.

Alleviation of exogenous oligochitosan on wheat seedlings growth under salt stress.

Hydroponic experiments were carried out to study the role of oligochitosan in enhancing wheat (Triticum aestivum L.) resistance to salt stress. Data were collected on plant biomass, chlorophyll content, photosynthetic rate (P (n)), stomatal conductance (g (s)), proline content, antioxidant enzyme activities, and malondialdehyde (MDA) content.

Elevated carbon dioxide and/or ozone concentrations induce hormonal changes in Pinus tabulaeformis.

We investigated endogenous plant hormones and needle growth in Pinus tabulaeformis plants grown in open-top chambers and exposed to ambient or elevated concentrations of carbon dioxide (CO(2)) and/or ozone (O(3)). Exposure to elevated CO(2) for 100 days significantly increased the change in fresh needle weight, indole-3-acetic acid (IAA), isopentenyl-adenosine (iPA), and dihydrozeatin riboside (DHZR) content. Abscisic acid (ABA) content decreased, and no effect was observed on zeatin riboside (ZR) content or changes in needle dry weight.

[Hydrolysis technology optimization of phorbol esters by orthogonal experiment].

Objective: To establish the best hydrolytic conditions from phorbol esters.

Method: The orthogonal experiment was used to optimize 4 factors, which were reaction time, ratio of solid-to-liquid, hydrolytic times, and temperature. Diamonsil C18 column (4.

[Study on the sorption and isolation of shikimic acid by 717 anion exchange resin].

Objective: To establish a convenient, practical and environmental method for extracting and isolating shikimic acid.

Methods: The content of shikimic acid was measured by RP-ion-pair HPLC, the effects of different pH, temperature, sample concentration and eluate concentration on 717 anion exchange resin were studied.

Results: The yield of shikimic acid was 96.

[Cu and Pb absorption and tolerance of Agrostis stolonifera and Festuca arundinacea].

This paper studied the seed germination rate, chlorophyll content, net photosynthetic rate, SOD activity, and Cu and Pb absorption of Agrostis stolonifera and Festuca arundinacea under Cu and Pb pollution. The results showed that Cu and Pb pollution had a significant effect on the seed germination rate of F. arundinacea.

[Specific promoters used in plant gene engineering].

The choice of specific promoters used within a transgene construct is a vital strategy to achieve the transgene regulation in the temporal, spatial and measurable manner. The strategy has been widely used in diverse aspects of plant gene engineering, such as quality improvement, resistance breeding and bioreactor. In this paper, we describe the structure feature, classification and research method of the specific promoter and its application progresses in plant gene engineering.