Three-dimensional reconstruction of rat sperm using volume electron microscopy.

Three-dimensional (3D) reconstruction serves as a crucial instrument for the analysis of biological structures. In particular, a comprehensive and accurate 3D ultrastructural examination of rat sperm is vital for understanding and diagnosing male fertility issues and the underlying causes of infertility. In this study, we utilize the automated tape-collecting ultramicrotome scanning electron microscopy (ATUM-SEM) imaging technique, which is a highly effective method for 3D cellular ultrastructural analysis.

Quantification of meropenem in serum and cerebrospinal fluid in children with bacterial meningitis with augmented renal clearance by UPLC-MS/MS.

Meropenem is an ultrabroad-spectrum antimicrobial agent that is often recommended for the treatment of bacterial meningitis (BM) in children. However, a subtherapeutic phenomenon occurred in BM children complicated with augmented renal clearance (ARC) at the recommended dose of meropenem. To support its pharmacokinetics, a sensitive, fast and robust ultra-liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was developed to measure meropenem concentrations in serum and cerebrospinal fluid (CSF).

Powerful-IoU: More straightforward and faster bounding box regression loss with a nonmonotonic focusing mechanism.

Bounding box regression (BBR) is one of the core tasks in object detection, and the BBR loss function significantly impacts its performance. However, we have observed that existing IoU-based loss functions suffer from unreasonable penalty factors, leading to anchor boxes expanding during regression and significantly slowing down convergence. To address this issue, we intensively analyzed the reasons for anchor box enlargement.

Fear memory-associated synaptic and mitochondrial changes revealed by deep learning-based processing of electron microscopy data.

Serial section electron microscopy (ssEM) can provide comprehensive 3D ultrastructural information of the brain with exceptional computational cost. Targeted reconstruction of subcellular structures from ssEM datasets is less computationally demanding but still highly informative. We thus developed a region-CNN-based deep learning method to identify, segment, and reconstruct synapses and mitochondria to explore the structural plasticity of synapses and mitochondria in the auditory cortex of mice subjected to fear conditioning.

Spatial optimization mode of China's rural settlements based on quality-of-life theory.

This paper studies the optimization mode of the spatial organization of rural settlements based on the two-way interactive mechanism between the space of rural settlements and quality-of-life theory. It examines three main aspects: the integration of spatial function, the optimization of spatial structure, and the regulation of spatial scale. In this paper, we built an optimization mode and framework of spatial organization, called the road-oriented mode of rural settlements, based on quality-of-life theory.

[Pathogens and risk factors for ventilator-associated pneumonia in children with congenial heart disease after surgery].

Objective: To investigate the distribution and drug sensitivity of pathogens and risk factors for ventilator-associated pneumonia (VAP) in children with congenial heart disease (CAD) after surgery.

Methods: According to the occurrence of VAP, 312 children with CAD who received mechanical ventilation after surgery for 48 hours or longer between January 2012 and December 2014 were classified into VAP (n=53) and non-VAP groups (n=259). Sputum samples were collected and cultured for pathogens in children with VAP.

An efficient approach for production of polystyrene/poly(4-vinylpridine) particles with various morphologies based on dynamic control.

Polystyrene/poly(4-vinylpyridine) (PS/P4VP) particles with various morphologies such as popcorn-like (A), colloidal molecule NH(3) (B), colloidal molecule H(2)O (C) and mushroom-like (D) were generated by dynamically controlling and stabilizing the phase separation during the seeded swelling polymerization.

[Study on the effects of cool and hot ethanol extracts from qinglongyi on the biochemical function of tumor membrane in H22 mice].

Objective: To study the effects of cool and hot ethanol extracts from Qinglongyi on tumor membrane protein content, lipid fluidity, and membrane close capability in H22 mice.

Method: The membrane protein content, by, lipid fluidity and membrane close capability were measured by means of SDS-PAGE, skinitzky assay and Zamudio method respectively.

Result: The cool and hot ethanol extracts from Qinglongyi decreased the content of tumor membrane protein, the lipid fluidity and membrane close capability.