Deacetylated SNAP47 recruits HOPS to facilitate autophagosome-lysosome fusion independent of STX17.

Autophagy, a conserved catabolic process implicated in a diverse array of human diseases, requires efficient fusion between autophagosomes and lysosomes to function effectively. Recently, SNAP47 has been identified as a key component of the dual-purpose SNARE complex mediating autophagosome-lysosome fusion in both bulk and selective autophagy. However, the spatiotemporal regulatory mechanisms of this SNARE complex remain unknown.

The neuroimmune mechanism of pain induced depression in psoriatic arthritis and future directions.

Patients suffering from psoriatic arthritis (PsA) often experience depression due to chronic joint pain, which significantly hinders their recovery process. However, the relationship between these two conditions is not well understood. Through a review of existing studies, we revealed that certain neuroendocrine hormones and neurotransmitters are involved in the neuroimmune interactions related to both PsA and depression.

Bridged emulsion gels from polymer-nanoparticle enabling large-amount biomedical encapsulation and functionalization.

Large-amount encapsulation and subsequent expressing are common characteristics for many biomedical applications, such as cosmetic creams and medical ointments. Emulsion gels can accomplish that, but often undergo exclusive, complex, multiple synthesis steps, showing extremely laborious and non-universal. The method here is simple via precisely interfacial engineering in homogenizing a nanoparticle aqueous dispersion and a polymer oil solution, gaining interfacial 45° three-phase-contact-angle for the nanoparticle that can bridge across oil emulsions' interfaces and ultimately form interconnected macroscopic networks.

Mitigation of irreversible membrane biofouling by CNTs-PVDF conductive composite membrane.

Biofouling has been one of the major challenges impacting the long-term stable operation of ultrafiltration processes. Irreversible biofouling is considerably more harmful than reversible biofouling. Conductive membrane, as a new technology to effectively mitigate membrane fouling, lack research of controlling irreversible biofouling.

Uneven heat burden in the sunshine state: Spatial patterns and socio-economic disparities of heat-related illness in Florida.

Climate change has increased the frequency and severity of extreme heat events globally, adversely affecting socio-economic conditions and public health. However, extreme heat has disparate effects on different population groups and the socio-economic determinants of its health effects are not well understood. In this study, we analyzed the spatial patterns of heat-related illness (HRI) visit rates at the zip-code level in Florida and applied statistical methods to examine the relationships between HRIs and environmental and socio-economic variables.

Confined Synthesis of 2D Molybdenum Diphosphide Nanosheets via Gas-Solid Transformation.

Molybdenum diphosphide (MoP), a topological semimetal, possesses distinctive properties and applications in catalysis, energy storage, and condensed matter physics. However, synthesizing high-purity MoP is complex and often results in undesired stoichiometric by-products. Additionally, the intrinsic orthorhombic crystal structure makes it difficult to synthesize MoP in a 2D morphology, which is desirable for device and energy applications.

Unveiling the critical role of overlooked consumer protist-bacteria interactions in antibiotic resistance gene dissemination in urban sewage systems.

Antibiotic resistance genes (ARGs) are emerging contaminants of significant concern due to their role in facilitating the spread of antibiotic resistance, especially high-risk ARGs, which are characterized by high human accessibility, gene mobility, pathogenicity, and clinical availability. Studies have shown that cross-domain interactions, such as those between consumer protists (consumers) and bacteria, can influence bacterial diversity, distribution, and function through top-down control. The consumers-bacteria interactions may also affect the occurrence and distribution of ARGs, yet this has been scarcely explored in field investigations.

Gestational exposure to arsenic reduces female offspring fertility by impairing the repair of DNA double-strand breaks and synapsis formation in oocytes.

Arsenic is a pollutant that can cross the placenta; however, research on the effects of arsenic exposure during pregnancy on the fertility of female offspring is limited. To address this gap, we developed a mouse model to investigate the relationship between arsenic exposure during pregnancy and fertility in female offspring. Our fertility assessment revealed that gestational exposure to 1 mg/kg arsenic or higher (10 mg/kg) resulted in reduction in litter size, ovarian volume, and multistage-follicle number in female offspring.

Scalable deoxygenative alkynylation of alcohols via flow photochemistry.

Internal alkynes are often contained in bioactive pharmaceuticals and crucial intermediates in material sciences, yet their production methods are often limited and challenging, necessitating the development of more efficient and versatile synthetic routes. Here we report a method of deoxygenative alkynylation of alcohols via flow photochemistry. Formation of N-heterocyclic carbene-alcohol adducts undergoes oxidation by a photocatalyst, generating alkyl radicals.

Identifying the Role of Interfacial Long-Range Order in Regulating the Solid Electrolyte Interphase in Lithium Metal Batteries.

The self-assembled monolayer (SAM) technique, known for its customizable molecular segments and active end groups, is widely recognized as a powerful tool for regulating the interfacial properties of high-energy-density lithium metal batteries. However, it remains unclear how the degree of long-range order in SAMs affects the solid electrolyte interphase (SEI). In this study, we precisely controlled the hydrolysis of silanes to construct monolayers with varying degrees of long-range order and investigated their effects on the SEI nanostructure and lithium anode performance.

Tumour evolution and microenvironment interactions in 2D and 3D space.

To study the spatial interactions among cancer and non-cancer cells, we here examined a cohort of 131 tumour sections from 78 cases across 6 cancer types by Visium spatial transcriptomics (ST). This was combined with 48 matched single-nucleus RNA sequencing samples and 22 matched co-detection by indexing (CODEX) samples. To describe tumour structures and habitats, we defined 'tumour microregions' as spatially distinct cancer cell clusters separated by stromal components.

Corrigendum: Effectiveness, safety, and treatment pattern of sodium zirconium cyclosilicate in Chinese patients with hyperkalemia: interim analysis from a multicenter, prospective, real-world study (Actualize Study).

[This corrects the article DOI: 10.3389/fphar.2024.

Synthesis of 1,2-Benzothiazine via Nickel-Catalyzed Electrochemical Intramolecular Amination.

Constructing a C-N bond by merging electrochemistry and nickel catalysis is considered a powerful strategy. Herein, we investigate highly efficient intramolecular amination at room temperature with excellent functional group tolerance. Mechanistic studies suggest that the rapid ligand exchange may lead to the Ni/Ni catalytic cycle.

Methylmercury chloride inhibits meiotic maturation of mouse oocytes in vitro by disrupting the cytoskeleton.

Methylmercury chloride (MMC) is a persistent heavy metal contaminant that can bioaccumulate in humans via the food chain, exerting detrimental effects on health. Nevertheless, the specific influence of MMC on oocyte meiotic maturation has yet to be elucidated. This research demonstrated that MMC exposure during the in vitro cultivation of mouse oocytes did not influence germinal vesicle breakdown but markedly decreased oocyte maturation rates.

Study on the Polymer Morphology and Electro-Optical Performance of Acrylate/Epoxy Resin-Based Polymer-Stabilized Liquid Crystals Based on Stepwise Photopolymerization.

Stepwise photopolymerization is a miraculous strategy modulating the polymer skeleton and electro-optical properties of light modulators based on liquid crystal/polymer composites. However, owing to the indistinct polymerization mechanism and curing condition discrepancy, the required polymer structures and electro-optical properties are hard to be controlled precisely. Herein, a novel polymer-stabilized liquid crystal film based on acrylate/epoxy resin is proposed, fabricated and the relationships between preparation process, polymer content, polymer morphology and electro-optical properties are studied.

Emerging Two-Dimensional Materials for Proton-Based Energy Storage.

The rapid diffusion kinetics and smallest ion radius make protons the ideal cations toward the ultimate energy storage technology combining the ultrafast charging capabilities of supercapacitors and the high energy densities of batteries. Despite the concept existing for centuries, the lack of satisfactory electrode materials hinders its practical development. Recently, the rapid advancement of the emerging two-dimensional (2D) materials, characterized by their ultrathin morphology, interlayer van der Waals gaps, and distinctive electrochemical properties, injects promises into future proton-based energy storage systems.

Discovery of Inhibitors Targeting Protein-Protein Interaction between Bacterial RNA Polymerase and NusG as Novel Antimicrobials.

Bacterial RNA polymerase (RNAP), the core enzyme responsible for bacterial transcription, requires the NusG factor for efficient transcription elongation and termination. As the primary binding site for NusG, the RNAP clamp-helix (CH) domain represents a potential protein-protein interaction (PPI) target for novel antimicrobial agent design and discovery. In this study, we designed a pharmacophore model based on the essential amino acids of the CH for binding to NusG, such as R270, R278, and R281 ( numbering), and identified a hit compound with mild antimicrobial activity.

The microbiota continuum along the upper reproductive tract of male rat and its relation to semen parameters.

Given the physiological function and anatomical location of the reproductive tract, studying the upper reproductive tract microbiota may be essential for studying male infertility and other male diseases. This study aimed to characterize the microbiota of the upper reproductive tract male rats and investigate whether specific microbial compositions are associated with sperm parameters. 16S rRNA gene sequencing was used to characterize the microbial composition in the testis, epididymis, seminal vesicles, vas deferens and prostate tissues of the rats.