The development of a dietary nutrient density educational tool and the investigation of its acceptance by Chinese residents from Henan province.

Objectives: Helping residents select nutrient-dense foods is a strategy to improve their diet quality. However, communication based on the nutrient-dense foods as a positive attribute has not been widely used in nutritional education. This study aimed to develop an educational tool based on the picture and guidance of "Chinese food guide pagoda (2022) ", extend it with the concept of nutrient density, and investigate its acceptance by Chinese residents from Henan province.

Targeted Therapy of Osteoarthritis via Intra-Articular Delivery of Lipid-Nanoparticle-Encapsulated Recombinant Human FGF18 mRNA.

Fibroblast growth factor 18 (FGF18) emerges as a promising therapeutic target for osteoarthritis (OA). In this study, a novel articular cavity-localized lipid nanoparticle (LNP) named WG-PL14 is developed. This optimized formulation has a nearly 30-fold increase in mRNA expression as well as better articular cavity enrichment compared to commercial lipids MC3 when performing intra-articular injection.

Robotic Fast Dual-Arm Patch Clamp System for Mechanosensitive Excitability Research of Neurons.

Objective: A robotic fast dual-arm patch clamp system with controllable mechanical stimulation is proposed in this paper for mechanosensitive excitability research of neurons in brain slice.

Methods: First, a kinematic model of a dual-arm patch clamp system combined with Monte Carlo method is developed to calculate the workspaces of recording micropipette and stimulation micropipette, and optimize the length of end effector for reducing collision incidences during operation. Then, a quantitative stimulation method to cells using one micropipette is developed based on pressing depth control.

Effect of body mass index on ovarian reserve and ART outcomes in infertile women: a large retrospective study.

Background: Obesity poses a significant global health challenge, with profound implications for women's reproductive health. The relationship between ovarian reserve and body mass index (BMI) remains a subject of debate. While obesity is generally associated with poorer outcomes in assisted reproductive technology (ART), the evidence remains inconclusive.

Modulation of liver metabolism and gut microbiota by Alhagi-honey alleviated heat stress-induced liver damage.

Alhagi-honey (AH) is a well-established traditional ethnic medicine with advantageous effects against diarrhea and headaches. We aimed to explore the preventive effect of AH on liver damage induced by heat stress (HS) and its underlying mechanism. HS models were established by thermostat, and mice were treated at 39 ℃ for 10 h, lasting for 7 days.

Modulating DNA Polα Enhances Cell Reprogramming Across Species.

As a fundamental biological process, DNA replication ensures the accurate copying of genetic information. However, the impact of this process on cellular plasticity in multicellular organisms remains elusive. Here, we find that reducing the level or activity of a replication component, DNA Polymerase α (Polα), facilitates cell reprogramming in diverse stem cell systems across species.

A novel online calculator based on inflammation-related endotypes and clinical features to predict postoperative pulmonary infection in patients with cervical spinal cord injury.

Background: Postoperative pulmonary infection (POI) of patients with cervical spinal cord injury (CSCI) is highly heterogeneous, while the potential endotypes and related risk factors remain unclear.

Methods: A retrospective collection of 290 CSCI patients was conducted from January 2010 to July 2024 using 1:1 propensity score matching to compare POI (n = 145) and non-POI (n = 145) groups. We generated laboratory examination data from admission patients and identified endotypes using unsupervised consensus clustering and machine learning.

GRNMOPT: Inference of gene regulatory networks based on a multi-objective optimization approach.

Background And Objective: The reconstruction of gene regulatory networks (GRNs) stands as a vital approach in deciphering complex biological processes. The application of nonlinear ordinary differential equations (ODEs) models has demonstrated considerable efficacy in predicting GRNs. Notably, the decay rate and time delay are pivotal in authentic gene regulation, yet their systematic determination in ODEs models remains underexplored.

Pathological Involvement of Protein Phase Separation and Aggregation in Neurodegenerative Diseases.

Neurodegenerative diseases are the leading cause of human disability and immensely reduce patients' life span and quality. The diseases are characterized by the functional loss of neuronal cells and share several common pathogenic mechanisms involving the malfunction, structural distortion, or aggregation of multiple key regulatory proteins. Cellular phase separation is the formation of biomolecular condensates that regulate numerous biological processes, including neuronal development and synaptic signaling transduction.

Genome-Wide Association Study of Resistance to Largemouth Bass Ranavirus (LMBV) in .

The disease caused by Largemouth bass ranavirus (LMBV) is one of the most severe viral diseases in largemouth bass (). It is crucial to evaluate the genetic resistance of largemouth bass to LMBV and develop markers for disease-resistance breeding. In this study, 100 individuals (45 resistant and 55 susceptible) were sequenced and evaluated for resistance to LMBV and a total of 2,579,770 variant sites (SNPs-single-nucleotide polymorphisms (SNPs) and insertions-deletions (InDels)) were identified.

The Synergistic Effect of Calcained Coal-Series Kaolinite and Limestone on the Hydration of Portland Cement.

Limestone calcined clay cement (LC) presents a promising alternative material due to its reduced CO emissions and superior mechanical properties compared to traditional Portland cement (PC). This study investigates the synergistic effect of calcined coal-series kaolinite (CCK) and limestone (LS) on the hydration behavior of cement, specifically focusing on varying mass ratios. The combination of CCK and LS promotes the formation of strätlingite and carboaluminates, which enhances early-age strength development.

Optimizing Hearing Outcomes in Nasopharyngeal Cancer Survivors in the Era of Modern Radiotherapy and Systemic Therapy.

Intensity-modulated radiation therapy (IMRT) improves disease control and reduces treatment-related toxicity in patients with localized nasopharyngeal carcinoma (NPC). However, due to the proximity of the auditory apparatus to the treatment volume and the frequent incorporation of cisplatin-based chemotherapy, treatment-related sensorineural hearing loss (SNHL) remains a common debilitating complication among NPC survivors. The reported crude incidence of SNHL following IMRT for NPC varies widely at 1-46% due to differences in auditory assessment methods and thresholds, follow-up durations, chemotherapy usage, and patient compositions.

Lactone-to-Lactam Editing Alters the Pharmacology of Bilobalide.

Precise transformations of natural products (NPs) can fine-tune their physicochemical properties while preserving inherently complex and evolutionarily optimized parent scaffolds. Here, we report an unprecedented lactone-to-lactam transformation on bilobalide, thus improving its stability and paving the way for biological exploration of previously inaccessible chemical space that is highly representative of the parent structure. This late-stage molecular editing of bilobalide enables facile access to a unique library of lactam analogues with altered pharmacology.

Unraveling the mysteries of early embryonic arrest: genetic factors and molecular mechanisms.

Early embryonic arrest (EEA) is a critical impediment in assisted reproductive technology (ART), affecting 40% of infertile patients by halting the development of early embryos from the zygote to blastocyst stage, resulting in a lack of viable embryos for successful pregnancy. Despite its prevalence, the molecular mechanism underlying EEA remains elusive. This review synthesizes the latest research on the genetic and molecular factors contributing to EEA, with a focus on maternal, paternal, and embryonic factors.

Enhanced Photocatalytic Hydrogen Production Activity Driven by TiO/(MoP/CdS): Insights from Powder Particles to Thin Films.

Transitioning from powder photocatalysts to thin film photocatalysts is one of the necessary steps toward industrializing photocatalytic hydrogen production. Herein, we reported the integration of non-noble metal cocatalyst MoP decorated with TiO and CdS, forming TiO/(MoP/CdS) for ultraviolet-visible light utilization. The designed powder TiO/(MoP/CdS) composites achieved a superior hydrogen production rate of 42.

DJ-1 regulates mitochondrial function and promotes retinal ganglion cell survival under high glucose-induced oxidative stress.

Purpose: This study aimed to investigate the antioxidative and neuroprotective effects of DJ-1 in mitigating retinal ganglion cell (RGC) damage induced by high glucose (HG).

Methods: A diabetic mouse model and an HG-induced R28 cell model were employed for loss- and gain-of-function experiments. The expression levels of apoptosis and oxidative stress-related factors, including Bax, Bcl-2, caspase3, Catalase, MnSOD, GCLC, Cyto c, and GPx-1/2, were assessed in both animal and cell models using Western blotting.

Long-Term Survival of Implants Placed in Earlier Failed Implant Sites.

Dental implant therapy is generally successful. However, when such therapy fails, considerations for implant replacement must be carefully considered. The survivability of implants placed into previously implanted sites must be considered.

Mitochondrial-Derived Peptide MOTS-c Suppresses Ovarian Cancer Progression by Attenuating USP7-Mediated LARS1 Deubiquitination.

Mitochondrial-nuclear communication plays a vital role in maintaining cellular homeostasis. MOTS-c, a short peptide derived from the 12S rRNA of mitochondrial DNA, has been suggested as a retrograde mitochondrial signal. Although recent clinical studies have suggested a possible link between MOTS-c and human cancer, the role of MOTS-c in tumorigenesis has yet to be investigated.

Effects and individual response of salt substitute intervention on blood pressure in a high-risk stroke population: findings from 4200 participants of the China Salt Substitute and Stroke Study.

: The protective effects of salt substitutes on blood pressure are well established, yet the individual variations in response to salt substitutes remain unclear. Our study aims to identify the individuals who derive the greatest benefit from salt substitute interventions. : Our study involved 4200 participants at high-risk of stroke from 120 villages in Liaoning Province, as a sub-study of the Salt Substitute and Stroke Study (SSaSS) trial, with 60 villages receiving a 5-year salt substitute intervention and other 60 villages using regular salt.

Biofilm inactivation using LED systems emitting germicidal UV and antimicrobial blue light.

Biofilms have been widely detected in water distribution and water storage systems posing potential risks to drinking water safety by harboring and shedding pathogens. Light-based disinfection methods, such as germicidal ultraviolet (UV) and antimicrobial blue light (aBL), could serve as non-chemical alternatives for biofilm control. This study investigated the inactivation of pure-culture Pseudomonas aeruginosa biofilms and mixed-culture biofilms using three distinct light-based disinfection methods: a low-pressure (LP) UV lamp emitting at 254 nm, a UV light emitting diode (LED) at 270 nm, and an aBL LED at 405 nm.