[Role of nitric oxide in the mechanisms of formation of reflex vasomotor responses].

This review focuses on modem data concerning the role of nitric oxide (NO) in the mechanisms of vasomotor regulation. On the background of the literature data and own experimental results, we have discussed some questions of NO integration into transmission of impulses in central and autonomic nervous system under condition of realization of cardiogenic and sinocarotid pressor and depressor reflexes, reflectory vasomotor responses formation under acute myocardial ischemia. According to literature and own functional and morphological data we suggest species differences in NO participations in mechanisms of reflex self--regulation of circulation.

[Sources of cortical, hypothalamic and spinal serotoninergic projections: topical organization in the dorsal Raphe nucleus].

Distribution of primuline, fast blue, fluoro-gold and nuclear yellow-labelled monoamine-containing cells in periventricular gray and dorsolateral tegmentum (including locus coeruleus) was studied in the rat after injection of these fluorochromes into the frontal cortex, hypothalamus and spinal cord. Combination of monoamine fluorescence method and retrograde cell-labelling was used. Two big groups of serotonin-positive cells projecting into the upper thoracic spinal segments were found in dorsomedial zone of the dorsal raphe.

[The bevelling of microelectrodes by vibration without using a special generator].

An original set-up replacing generator of sound frequencies is suggested. This set-up connected with AC current of 50 Hz frequency can be used as a power source to the electromagnetic vibrator with the frequency of the mechanical resonance of 200 Hz.

[Correlations between thalamic projections to different areas of the parietal association cortex of the cat].

Projections of thalamic neurons to parietal association cortex of cat were examined by means of the retrograde axonal transport of fluorescent dyes (primuline and fast blue). It has been demonstrated that a dorsal part of the pulvinar (PL) and a dorsal part of the caudal area of the lateral posterior nucleus (LP) projected mostly to the middle suprasylvian gyrus (MSSG), while a ventral part of PL and a ventral part of the rostral area of LP--to the rostral suprasylvian gyrus (RSSG). Double labelled neurons were found in PL, LP, suprageniculatus, anterior ventral, ventral lateral as well as in the central lateral, paracentral and central medial nuclei after injections of two different dyes into MSSG and RSSG.

[Ultrastructural characteristics of callosal neurons in deep layers of the primary auditory cortex (AI) in cat].

An electron microscope study of retrogradely labelled nonpyramidal neurons has been carried out in layers V-VI of the primary auditory cortex (AI) after HRP injections into the contralateral AI of cats. From 2 to 9 synapses were usually revealed on somatic profiles of these callosal neurons. Synapses occupied 15.

[A method of determining glucose-6-phosphate dehydrogenase in bacteria of the genus Yersinia].

A method for qualitative assessment of glucose-6-phosphate dehydrogenase in Yersinia is suggested. The reaction mixture was prepared by mixing the ingredients in the ratios as follows: 10 microliters of 0.01 M glucose-6-phosphate, 10 microliters of 0.

[The ultrastructural characteristics of the pyramidal callosal neurons of layer III in the primary auditory area (A1) of the cat cortex].

An electron microscope study of retrogradely labelled pyramidal neurons in layer III of the primary auditory cortex (AI) after HRP injections into the contralateral AI has been carried out in cats. From 4 to 10 synapses were usually revealed on somatic profiles of these callosal neurons. Synapses occupied 20.

[The morphological characteristics of the callosal neurons of the first auditory area of the cortex (AI) in the cat].

Cortical stratification of callosal neurons in the primary auditory cortex (AI) of cat was studied by means of horseradish peroxidase (HRP). Two main groups of callosal neurons were revealed. The first group comprising 60% of all AI callosal neurons consisted predominantly of layer III large pyramidal neurons.

[Identification of catecholamines in the rat brain and mollusk tissues using semithin slices and retrograde staining].

The suggested technique allows revealing the transport-specific dye (primulin) and catecholamine fluorescence simultaneously in the same cell of brain. Intense fluorescence is observed when brain tissue is quickly dehydrated and embedded in the epoxy resin. The same method is suggested for the identification of catecholamines in the embryonal and juvenile tissues of gastropod Lymnaea stagnalis (Mollusca Pulmonata) without using of primulin dye.

[A method of rapid beveling of microelectrodes using vibration].

The new technique is suggested for a rapid bevelling of glass and metallic microelectrodes by means of ends' friction on the fine-grained surface of a quartz plate vibrating at a frequency of the mechanical resonance of the system.

[Thymine- and thymidine-dependence of Yersinia pestis mutants with altered thymidylate synthetase].

Thymine- and thymidine-dependent mutants of Y. pestis strain EV-76 have been isolated and characterized. Obtaining Y.

[Distribution of norepinephrine-containing neurons projecting to the parietal associative cortex and spinal cord in the cat locus coeruleus].

The distribution of neurons projecting to the parietal associative cortex and spinal cord in the locus coeruleus (LC) of the cat was examined by the retrograde transport of the horseradish peroxidase (HRP) and catecholamine histofluorescence technique. It was demonstrated that neurons projecting to the parietal cortex were localized predominantly in the dorsal LC and the most dense labeling was observed at the coronal section corresponding to frontal plane P-1. Neurons projecting to the spinal cord were concentrated in the ventral LC and the largest quantity of labeled neurons was found in the frontal plane P-3.

[Brain stem neurons of the guinea pig that form projections into the ampullae of the anterior, lateral and posterior semicircular canals].

Brain stem neurons projecting to the ampullae of anterior, lateral and posterior semicircular canals (vestibular efferent neurons) were identified in guinea pigs using, retrograde labelling with horseradish peroxidase. Two groups of such neurons located bilaterally dorsal and ventral to the facial nerve trajectory were classified. A dorsal group contained vestibular efferent neurons to all three canals; these units were located at all the levels of the subependymal granular layer of the floor of the 4th ventricle lateral to the facial nerve genu and abducens nerve nucleus.

[Distribution in the diencephalon of the rat of retrogradely primuline-labelled dopamine-containing neurons forming spinal projections].

Distribution of primuline-labeled dopamine-containing neurons in the rat diencephalon following injection of fluorochrome into upper thoracic spinal cord was studied using a combination of catecholamine fluorescence method and retrograde cell-labeling. It was shown that only neurons of group A11 located in the dorsal hypothalamus and caudal thalamus give rise to the spinal projections. Non-catecholamine cells were also labeled in this region.

[Purine and pyrimidine metabolism in Francisella tularensis].

The assimilation and mutual transformation of exogenous purine and pyrimidine bases and their nucleosides in the known subspecies of F. tularensis have been studied by means of radio-labeled compounds. The possibility of using the specific features of the metabolism of these compounds in F.