[Biochemical markers of toxic and mutagenic complications of cytostatic pharmacotherapy].

Data are submitted on individual prophylaxis of damage to the genetical apparatus of bodily normal cells in cytostatic therapy of immunodeficiency states by observing changes in biochemical indices, such as activity of enzymes, content of biologically active substances and hormones.

[Protracted atypical course of enterovirus infection].

Two cases are presented of replication of enteroviruses over a long period of time, that caused a protracted atypical clinical course of infection. No typical clinical markers of infection can be seen in the wake of enterovirus replication; the replication itself can be of a protracted recurrent character. Replication of enteroviruses is capable of inducing a protracted recurrent subfebrile condition in adults that has no clinical signs of immunodeficiency state.

[The relationship of the intensity of antibody formation to the causative agents of diphtheria and tetanus to genetic markers in children vaccinated with adsorbed DPT].

The examination of 99 children immunized with adsorbed DPT vaccine revealed that the intensity of immune response to the diphtheria and tetanus components of the preparation were linked with a number of genetic markers (respectively, HLA-A9, B35, etc.; HLA-A25, B8, etc.) and their combinations.

[A micromodification of the determination of specific leukocyte lysis in vitro].

A micromodification of the specific leukocyte lysis method was developed; only 0.02 ml of blood is sufficient for the test. The micromodification improved the reproducibility of the technique more than twofold, simplified it, and is recommended for examinations of some populations, including children whose body reactivity is changed and an individual approach to their immunization with TDP vaccine is necessary.

[A method of separating mononuclears on a density gradient].

The developed procedure for the isolation of mononuclears in density gradient d = 1.0077 g/cm3 consists in separation of the plasma by preliminary centrifugation at 200 g for 7-10 min and isolation of leukocytic mononuclear cells from the cellular sediment diluted with the isotonic solution. The length of centrifugation at 400 g is selected with consideration for the thickness of the density gradient.

[Dehydrogenase activity in skeletal muscles of rats after long-term exposure to weightlessness].

Malate- and isocytratedehydrogenase activity in mitochondrial and cytoplasmic fractions and lactate dehydrogenase activity in hindlimb muscles have been studied at different stages after 18.5-day flight on a biosatellite "Cosmos-1129" and after 20-day hypokinesia. A decrease in dehydrogenase activity has been found on the first postflight day.

[Energy reactions in the skeletal muscles of rats after short-term space flight on Kosmos-1514].

Ten hours after the 5-day space flight on Cosmos-1514 rats were examined for oxidative phosphorylation in mitochondria isolated from the posterior femoral muscles as well as for Krebs cycle enzymes and glycolysis in the mitochondrial and cytoplasmic fractions of the muscles. The mitochondrial respiration rate in various metabolic states was similar in flight rats and vivarium controls. After flight calculated parameters of energy efficacy of respiration as well as activity of malate dehydrogenase, isocitrate dehydrogenase and total lactate dehydrogenase remained unchanged.

[Effectiveness of immunizing donors with adsorbed tetanus anatoxin in relation to their genetic markers].

Blood groups, including rhesus grouping, have been determined in 218 donors and HLA of loci A, B, C in 121 donors immunized with adsorbed tetanus toxoid (40 binding units). The presence of HLA Aw 32, B 8; HLA Bw 16, haplotypes A3 Bw 16 and A1, blank antigens of locus C is linked with the production of, respectively, high or low (less than or equal to 16 I.U.

[Energy reactions in the skeletal muscles of rats after a flight on the Kosmos-1129 biosatellite].

The polarographic analysis of biological oxidation in rat skeletal muscles after the 18.5-day flight revealed changes specific for the flight animals: oxidative phosphorylation uncoupling, distinct inertness of energy accumulation 10 hrs after recovery. Tissue respiration inhibition occurred in both flight and synchronous rats suggesting the effect of other than weightlessness factors.

[Energy reactions in the skeletal muscles of rats following space flight on the Kosmos-936 biosatellite].

The respiration of mitochondria isolated from mixed skeletal muscles of hindlimbs of rats flown for 18.5 days on Cosmos-936 was investigated polarographically. At R + 10 hours the rate of mitochondrial respiration in different metabolic states during the oxidation of succinic acid and NAD-dependent substrates declined.

[Skeletal muscle capillary bed during hypokinesia].

The functioning capillaries (containing red cells) of the gastrocnomius, soleus and tibial muscles as well as biceps femoris were subjected to quantitative histological study in rats exposed to 20-day hypokinesia. During hypokinesia the rats showed atrophy of and reduced blood supply to hind limb muscles as indicated by the decreased number of the functioning capillaries, the number of the capillaries supplying red muscular fiber being lowered in the first turn. The only exception was the soleus muscle where the number of the functioning capillaries remained unchanged, and blood congestion developed.