ASSOSIATION ANALYSYS OF 11 POLYMORPHISMS OF SNPS WITH ENDOTHELIUM DEPENDENT VASODILATATION IN CHILDREN WITH DIABETES MELLITUS TYPE 1.

We have studied the association with the level of the endothelium dependent vasodilatation (EDVD) among 11 single nucleotide polymorphisms (SNPs) of 10 genes in 45 children suffering from diabetes mellitus type 1. Following polymorphisms have been studied: G894→T of the eNOS exon 7 and Т-786→С of the eNOS promotor, А1266→G of the Eln exon 16, Т-381→C of the NPPB promotor, І\D of the ACE, Arg60→His of the LMP2, Met235→Thr of the AGT, A1166→C of the ATR1, C-1562→T of the MMP9, C-1306→T of the MMP2, and С-8→G of the PSMA6. It was shown that children with genotypes G/T by eNOS (G894→T), G/G by Eln (А1266→G), C/C by NPPB (Т-381→C) and І/D by ACE genes have lower EDVD (Р<0,05) than patients with others allelic variants of these genes, and this does not depend on duration of the disease, level of glicated hemoglobin and initial diameter of a humeral (brachial) artery.

[The prognostic significance of factors predisposing to the occurrence of pyelonephritis].

With the purpose of studying factors which might be pathogenetically interrelated with predisposition to pyelonephritis and used in clinical settings for diagnosis, the authors analyzed 275 records of pediatric patients having been examined in an in-patient facility for pyelonephritis. Clinical records of 106 children free from uronephrologic pathology were used as a comparison group. The examination was done by clinical, laboratory and biochemical, immunologic, functional and roentgenoradiologic techniques, as well as by making use of a medicogenetic approach.

[Fibronectin and its significance in pyelonephritis].

Fibronectin levels were measured by enzyme immunoassay in 68 patients with pyelonephritis and 10 patients with chronic cystitis. The patients with chronic cystitis, acute and chronic non-obstructive pyelonephritis exhibited significantly high average blood fibronectin levels, whereas those with chronic obstructive pyelonephritis displayed the levels slightly different from those observed in healthy individuals. Hypofibronectinemia was detected in 23.

[Problem of pathogenesis of Mycoplasma infection].

It has been shown that irrespective of the primary focus of affliction, M. hominis-induced infection should be regarded as a systemic disease characterized by an immune-mediated inflammation which is contributed to by blood kallikrein-kinin system activation resulting in changes in the coagulative system and fibrinolysis until disseminated intravascular coagulation develops. This allows one to interpret in a new fashion the etiology of some diseases proceeding with signs of vasculitis.

[A crystallographic method in the diagnosis of kidney diseases].

Structural aspects of copper chloride crystallization of the urine of patients with pyelonephritis and glomerulonephritis were studied by electron microscopy. It was found that admixtures of urea, creatinine, potassium and, possibly, sodium contained in the urine of patients initiate the formation of copper chloride crystals of different sizes, their shape changes, dendritic and spherolithic crystallization occurs. Results may be used as supplementary differential diagnostic signs of glomerulonephritis and pyelonephritis.

[Diagnostic possibilities of a crystallographic method in pyelonephritis in children].

Diagnostic potentialities of the crystallo-optic++ analysis of cupric chloride crystallographs++ were studied in 76 children with pyelonephritis and in 50 children with glomerulonephritis. The structural and morphological characteristics of cupric chloride crystallization under the influence of the urine from children with pyelo- and glomerulonephritis may serve as the additional differential-diagnostic signs of the diseases. The use of raster electron microscopy widens the diagnostic potentialities of the crystallographic method.

[Effect of interferon on the course of experimental E coli-induced pyelonephritis].

The effect of the type I interferon on the development and process of experimental pyelonephritis caused by E. coli was studied on mice weighing 12 to 14 g. Interferon was administered intraperitoneally in a dose of 1000 units on days 3 and 7 of the disease.

[Interferon in the modulation of the immune response in pyelonephritis caused by Staphylococci and Pseudomonas aeruginosa].

In experiments on mice the influence of mouse serum interferon, type I, on immune response in pyelonephritis caused by staphylococci and P. aeruginosa has been studied. The immunomodulating action of interferon and its therapeutic effectiveness have been shown to depend on the etiology of the disease.

[Immunoenzyme determination of fibrinogen and its degradation products in kidney diseases].

Immunoenzymatic assay (IEA) was used to study the content of fibrinogen and its splitting products (FSP) in patients with pyelonephritis and glomerulonephritis. The active phase of the disease was characterized by a marked increase of the fibrinogen content and FSP in the blood. The changes reflect the process of activation of the blood coagulation system with hypercoagulation phenomena.

[Chemiluminescence of blood and urine in children with pyelonephritis and glomerulonephritis].

It was established during the study of blood serum and urine chemiluminescence in 57 children with pyelonephritis and in 38 children with glomerulonephritis that in the active disease stage, the intensity of overfaint luminescence rises as a result of lipid peroxidation (LPO) activation, accumulation of lipid hydroperoxides and oxygen-containing radicals. Four types of the kinetic curves of urine chemiluminescence were identified. They characterize the correlation between LPO activation and the level of antiradical defence in patients suffering from pyelo- and glomerulonephritis.